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Plasmid#219644PurposePermits GoldenGate assembly of donor plasmids (BsmBI) to knock-in mScarlet:ALFA using the SEED/Harvest methodDepositorInsertmGreenLantern:ALFA SEED Cassette
UseTagsExpressionMutationPromoterAvailable sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHBJT001
Plasmid#225152PurposeHigh copy cloning vector for integration of C-terminal FLAG epitope tag. SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in same orientation as lac promoter.DepositorTypeEmpty backboneUseSynthetic BiologyTagsFLAG epitope tag (35 bp)ExpressionBacterialMutationPromoterAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCPH2A.X_S139D-GS3
Plasmid#204748PurposeExpression of Cas9 and human H2AX with the S139D mutationDepositorInsertH2AX S139D (H2AX Human)
UseTagsExpressionMammalianMutationH2AX with the S139D mutationPromoterAvailable sinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+)-Caprola_13
Plasmid#194674PurposeProtein production of the split HaloTag based calcium recorder Caprola_13 in bacteriaDepositorInsertCaprola_13
UseTagsHis-tag and Strep-tagExpressionBacterialMutationPromoterT7Available sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHA1.1-Rap2a-V5-Hygro
Plasmid#195510Purposelentiviral vector expressing RAP2A CDS with hygromycin selectionDepositorInsertHygromycin
UseLentiviralTagsExpressionMammalianMutationPromoterhPGKAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHyg-yeGFP-His6
Plasmid#212781PurposeC-terminal PCR-based tagging with yeGFP-His6 in budding yeast with HygR selectionDepositorTypeEmpty backboneUsePcr-based taggingTagsExpressionMutationPromoterAvailable sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDL17_Cas9-His Δcys M1C
Plasmid#206251PurposeBacterial expression of SpCas9 Δcys M1C variant under T7 promoterDepositorInsertSpCas9
UseCRISPRTags6x-His and SV40 NLSExpressionBacterialMutationM1C, C80S, C574SPromoterT7Available sinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-sCAG-eGFP-ACAP1 F280E
Plasmid#203801PurposeAAV vector plasmid expressing human ACAP1 mutation F280E fused to eGFP under a short variant of the CAG (sCAG) promoterDepositorInsertACAP1 (ACAP1 Human)
UseAAVTagseGFPExpressionMammalianMutationF280EPromoterAvailable sinceSept. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
HisTEV-cDAXX
Plasmid#197012PurposeE. coli expression vectorDepositorInsertDAXX
UseTags6His-TEVExpressionBacterialMutationPromoterAvailable sinceApril 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-EF1A-PBZC-Linker-eGFP
Plasmid#176070PurposeEGFP fused to the C-terminus of a PBZ domain & a hygromycin resistance cassetteDepositorInsertPBZ-C
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1AAvailable sinceFeb. 1, 2022AvailabilityAcademic Institutions and Nonprofits only