Addgene

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pJB066

Plasmid

#98913

Purpose

Express FtsZ tagged with pAmCherry1 in E coli

Depositor

Jie Xiao

Article

Buss et al PLoS Genet. 2015 Apr 7;11(4):e1005128. d

Insert

FtsZ

Use

Tags

pAmCherry1

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgUpf1

Plasmid

#161810

Purpose

guide RNA for Upf1

Depositor

Yi Zhang

Article

Fu et al Sci Adv. 2020 May 29;6(22):eaay5181. doi

Insert

sgRNA targeting mouse Upf1 (Upf1 Synthetic, Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pJB089

Plasmid

#98914

Purpose

Express Dronpa tagged ZapA and pAmCherry1 tagged FtsZ in E coli

Depositor

Jie Xiao

Article

Buss et al PLoS Genet. 2015 Apr 7;11(4):e1005128. d

Inserts

FtsZ
ZapA

Use

Tags

Dronpa and pAmCherry1

Expression

Bacterial

Mutation

Promoter

T5-lac

Available since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pAV0771

Plasmid

#133513

Purpose

Expression of mTagBFP2 tagged CHD domain to monitor actin cytoskeleton

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pAde6(PmeI)-p(act1)-3mTagBFP2-CHDRng2-terminator(ScCYC1)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFPC1-Sclt1

Plasmid

#128873

Purpose

For mammalian expression of mouse Sclt1

Depositor

Aimin Liu

Article

Ye et al Proc Natl Acad Sci U S A. 2014 Feb 11;11

Insert

Sclt1 (Sclt1 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pYpet-FsaA

Plasmid

#98279

Purpose

mammalian expression of Ypet-FsaA

Depositor

Mike Heilemann

Article

Finan et al Angew Chem Int Ed Engl. 2015 Oct 5;54(41

Insert

FsaA

Use

Tags

Ypet

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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pCfB4786

Plasmid

#106142

Purpose

EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked nourseothricin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntC_3, USER site for cloning, amp resistance

Depositor

Irina Borodina

Article

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pCO488

Plasmid

#139731

Purpose

Module plasmid, individual component of Fungal Bioluminescent Pathway, Hisps

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

pMOD_C'_CmYLCV:NnHisps:tHsp

Use

Luciferase

Tags

Expression

Plant

Mutation

Promoter

CmYLCV

Available since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pMC-1-35S_Long-6

Plasmid

#197698

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

35S promoter (1.3kb)

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-9-PinII-11

Plasmid

#197769

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

PinII terminator

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCI-neo-AP-4 (Myc)3-σ4

Plasmid

#198331

Purpose

Expresses the triple Myc-tagged mouse AP-4 σ4 subunit in mammalian cells

Depositor

Juan Bonifacino

Article

Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

Insert

AP-4 σ4

Use

Tags

triple Myc tag

Expression

Mammalian

Mutation

silent substitution in codon 131

Promoter

CMV

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pSBDEST.N

Plasmid

#79468

Purpose

Promoterless, Sleeping Beauty-compatible Gateway destination vector, Neomycin

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Type

Empty backbone

Use

Promoterless gateway destination vector

Tags

Expression

Mutation

Promoter

none

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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BASIC_7_J23101-RBS34-mCherry-B0015

Plasmid

#68141

Purpose

storage vector for BASIC part: RFP expression cassette

Depositor

Geoffrey Baldwin

Article

Storch et al ACS Synth Biol. 2015 Apr 16.

Insert

J23101-RBS34-mcherry-B0015

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pXJ40-Flag-iBox-PAK4cat

Plasmid

#190178

Purpose

expresses Flag-tagged iBox-PAK4cat

Depositor

Bianxiao Cui

Article

Li et al Nano Lett. 2019 Oct 9;19(10):6955-6963.

Insert

iBox-PAK4cat

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR2b-mVenus

Plasmid

#154898

Purpose

mVenus entry vector

Depositor

Johanna Ivaska

Article

Al-Akhrass et al Oncogene. 2021 Feb;40(7):1300-1317. doi:

Type

Empty backbone

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

July 27, 2020

Availability

Academic Institutions and Nonprofits only

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tISR

Plasmid

#122664

Purpose

Express tISR protein in E. coli

Depositor

Robert Cohen

Article

Choi et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

Insert

tISR (OCLM Human)

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

July 17, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pET-tTALE-EmGFP-15b

Plasmid

#138280

Purpose

Express tTALE-EmGFP

Depositor

Kyubong Jo

Article

Shin et al Sci Rep. 2019 Nov 20;9(1):17197. doi: 10

Insert

7-mer tTALE-EmGFP

Use

TALEN

Tags

6x His-tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

April 1, 2020

Availability

Academic Institutions and Nonprofits only

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pAV0624

Plasmid

#133491

Purpose

Expression of cytoplasmic 3mTagBFP2

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pUra4(AfeI)-p(tdh1)-3mTagBFP2-terminator(tdh1)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pGL2_174

Plasmid

#199106

Purpose

Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pNG2 ORI plus NGS barcode 22, gentamicin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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RRAS1

Plasmid

#55659

Purpose

expression of mouse RRAS1

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

RRAS1

Use

Tags

Expression

Bacterial

Mutation

Promoter

lac

Available since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only

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