Addgene

Showing: 6521 - 6540 of 6716 results

Results per page:

Sort results by:

0.41 hA33 luciferase

Plasmid

#68154

Purpose

luciferase reporter for hA33 promoter

Depositor

Joan Heath

Article

Johnstone et al J Biol Chem. 2002 Sep 13;277(37):34531-9

Insert

A33 (GPA33 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

starts at -405 relative to ATG

Promoter

human GPA33 promoter

Available since

Sept. 25, 2015

Availability

Academic Institutions and Nonprofits only
0.36 hA33 luciferase

Plasmid

#68155

Purpose

luciferase reporter for hA33 promoter

Depositor

Joan Heath

Article

Johnstone et al J Biol Chem. 2002 Sep 13;277(37):34531-9

Insert

A33 (GPA33 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

starts at -363 relative to ATG

Promoter

human GPA33 promoter

Available since

Sept. 25, 2015

Availability

Academic Institutions and Nonprofits only
0.38 hA33 luciferase

Plasmid

#68156

Purpose

luciferase reporter for hA33 promoter

Depositor

Joan Heath

Article

Johnstone et al J Biol Chem. 2002 Sep 13;277(37):34531-9

Insert

A33 (GPA33 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

starts at -384 relative to ATG

Promoter

human GPA33 promoter

Available since

Sept. 25, 2015

Availability

Academic Institutions and Nonprofits only
0.22 hA33 luciferase

Plasmid

#68157

Purpose

luciferase reporter for hA33 promoter

Depositor

Joan Heath

Article

Johnstone et al J Biol Chem. 2002 Sep 13;277(37):34531-9

Insert

A33 (GPA33 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

starts at -222 relative to ATG

Promoter

human GPA33 promoter

Available since

Sept. 25, 2015

Availability

Academic Institutions and Nonprofits only
mCherry

Plasmid

#176016

Purpose

Mammalian expression of mCherry. Parton lab clone DFQ

Depositor

Rob Parton

Article

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
DNA-PKcs T3950D

Plasmid

#83320

Purpose

DNA-PKcs T3950A

Depositor

Katheryn Meek

Article

Douglas et al Mol Cell Biol. 2007 Mar;27(5):1581-91. E

Insert

human DNA-PKcs (PRKDC Human)

Use

Tags

Expression

Mammalian

Mutation

Activation loop phosphorylation site 3950 substit…

Promoter

Available since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-pU6-(BbsI)-CcdB-(BbsI)-Pgk-Puro-T2A-BFP

Plasmid

#86457

Purpose

Mouse stem cell retroviral vector including a puromycin resistance and BFP gene. sgRNA targets can be cloned in between the BbsI sites

Depositor

Ralf Kuehn

Article

Chu et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

Insert

BFP

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Pgk

Available since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP

Plasmid

#176015

Purpose

Mammalian expression of EGFP. Parton lab clone DFS

Depositor

Rob Parton

Article

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJS647

Plasmid

#183176

Purpose

PYR1 mutagenesis plasmid compatible with pJS646 Y2H vector

Depositor

Timothy Whitehead

Article

Beltran et al Nat Biotechnol. 2022 Jun 20. pii: 10.103

Insert

PYR1 (PYR1 Mustard Weed)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pOT_2 - lenti-EFS-tNGFR-2A-puro

Plasmid

#181971

Purpose

Expresses human truncated NGFR linked to puromycin resistance via P2A for lentiviral delivery.

Depositor

Neville Sanjana

Article

Legut et al Nature. 2022 Mar;603(7902):728-735. doi:

Insert

tNGFR (NGFR Human)

Use

Lentiviral

Tags

Expression

Mutation

truncation: aa 11-276

Promoter

Available since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
WT Smarca4-V5 IRES-puro

Plasmid

#107058

Purpose

Expression of wild-type Smarca4 (Brg1)-V5 fusion with IRES puromycin resistance.

Depositor

Jerry Crabtree, Courtney Hodges

Article

Hodges et al Nat Struct Mol Biol. 2018 Jan;25(1):61-7

Insert

SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)

Use

Lentiviral

Tags

IRES-puroR and V5

Expression

Mammalian

Mutation

wild-type

Promoter

CAG

Available since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
WT Smarca4-sfGFP

Plasmid

#107056

Purpose

Expression of wild-type Smarca4 (Brg1) with C-terminal super-folder GFP fusion.

Depositor

Jerry Crabtree, Courtney Hodges

Article

Hodges et al Nat Struct Mol Biol. 2018 Jan;25(1):61-7

Insert

SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)

Use

Lentiviral

Tags

Super-folder GFP (sfGFP)

Expression

Mammalian

Mutation

wild-type

Promoter

CAG

Available since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV.CBA.YFP.miR-E_shControl

Plasmid

#180386

Purpose

Producing AAV that encodes negative control shRNA with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

Insert

shControl

Use

AAV and RNAi

Tags

Expression

Mutation

Promoter

CBA

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pT3-myr-AKT-HA

Plasmid

#31789

Depositor

Xin Chen

Article

Calvisi et al Gastroenterology. 2011 Mar;140(3):1071-8

Insert

Akt1 (Akt1 Mouse)

Use

Tags

HA and Myr

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 16, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
hyeA3A-BE4max

Plasmid

#157944

Purpose

Mammalian expression of hyeA3A-BE4max

Depositor

Dali Li

Article

Zhang et al Nat Cell Biol. 2020 Jun;22(6):740-750. d

Insert

hyeA3A-BE4max

Use

CRISPR

Tags

Expression

Mammalian

Mutation

N57G

Promoter

Available since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSAM2_mCherry_Baf60c

Plasmid

#72691

Purpose

Doxycyline regulated expression of Baf60c in mammalian cells

Depositor

Timothy Kamp

Article

Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

Insert

Brg 1 (SMARCD3 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV-Doxycycline

Available since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX-Puro-mGSDME

Plasmid

#154877

Purpose

Expresses mGSDME in mammalian cells

Depositor

Judy Lieberman

Article

Zhang et al Nature. 2020 Mar;579(7799):415-420. doi:

Insert

GSDME (Gsdme Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcsII-LMP7-flag

Plasmid

#86773

Purpose

C-terminal flag-tagged protein expression in mammalian cells, lentiviral vector

Depositor

Do-Hyung Kim

Article

Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

Insert

immunoproteasome beta subunit 5 (LMP7) (PSMB8 Human)

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pVC-FUS-R521C

Plasmid

#175067

Purpose

expresses mVenus and mCherry tagged to mutant of the FUS (R521C) protein in mammalian cells

Depositor

Arnold Boersma

Article

Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018

Insert

FUS glycine rich protein (FUS Human)

Use

Tags

HA and mVenus + mCherry

Expression

Mammalian

Mutation

changed Arginin 521 to Cystein; mVenus: C-Termina…

Promoter

CMV Promoter

Available since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
FUW-TetON-GFP

Plasmid

#115495

Purpose

Lentiviral plasmid for tet-inducible expression of GFP (generated from plasmid #20321)

Depositor

Catherine Verfaillie

Article

Garcia-Leon et al Stem Cell Reports. 2018 Feb 13;10(2):655

Insert

eGFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge