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Plasmid#118291PurposeExpresses human mutant DCX tagged with 2A peptide at it's C-terminus and mCherry in mammalian cells. Serine at position 306 substituted for alanineDepositorInsertDoublecortin (DCX Human)
UseAAVTags2A peptide and mCherryExpressionMammalianMutationchanged Serine 306 to AlaninePromoterCMV with beta global intronAvailable sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMARO
Plasmid#178086PurposeDerivative of pMK2010 that contains tetracycline resistance insert between attL1 and attL2 flanked by EcorV. Suitable as control or backbone for library construction for in vivo Gateway reaction.DepositorInsertTcR (tetA/tetR)
UseTagsExpressionBacterialMutationTetracycline resistance cassette is flanked by Ec…PromoterAvailable sinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-iCAP-AAV.CAP-B22
Plasmid#175005Purposenon-standard AAV2 rep-AAV.CAP-B22 cap plasmid with AAV cap expression controlled by a tTA-TRE amplifcation systemDepositorInsertSynthetic construct isolate AAV.CAP-B22 VP1 gene
UseAAVTagsExpressionMammalianMutation7 amino acid substitution between VP1 452 and VP1…Promoterp41Available sinceNov. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMK265 (RAD21-mAC Hygro)
Plasmid#140539PurposeRAD21 tagging with mAID-CloverDepositorInsertRAD21 (RAD21 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
WT-eGFP
Plasmid#215168PurposeEncodes the enhanced green fluorescent proteinDepositorInsertWT-eGFP
UseTagsHis-tagExpressionBacterialMutationPromoterT7 lacAvailable sinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJG103
Plasmid#158533PurposewrmScarlet11 x3 tandem repeatsDepositorInsertwrmScarlet11 x3 tandem repeats
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG CMV14 / BRAF-V600E
Plasmid#131723PurposeMammalian Expression of Flag tagged BRAFDepositorInsertBRAF (BRAF Human)
UseTags3xFLAGExpressionMammalianMutationV600EPromoterCMVAvailable sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSIN-PAmCherry-KFERQ-NE
Plasmid#102365PurposeLentiviral overexpression of PA-mCherry-KFERQ fusionDepositorInsertsPA-mCherry
KFERQ peptide
UseLentiviralTagsNEExpressionMammalianMutationPromoterEF-1aAvailable sinceDec. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SLCO4A1_STOP
Plasmid#161402PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLCO4A1 (SLCO4A1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
AAV-EF1A-DIO-PSD95.FingR-eGFP-CCR5TC
Plasmid#126216PurposeLabeling of excitatory synapses (green fluorescence) in cre-expressing neuronsDepositorInsertPSD95.FingR-eGFP-CCR5TC
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterEF1AAvailable sinceJuly 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pHrD-IRES-Fluc
Plasmid#194250PurposeHuman RNAP1 promoter region driving firefly luciferase expression from IRES. Constructed by Ghoshal, et al. J Biol Chem (2004). Please cite this original manuscript!DepositorInsertFirefly luciferase
UseLuciferaseTagsExpressionMutationPromoterhuman rDNA promoter and beginning of 5' ETS …Available sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTwist-SFFV-NFE2L1-Puro
Plasmid#181917PurposeLentiviral plasmid that directs the expression of NFE2L1/Nrf1 in mammalian cells.DepositorInsertNFE2L1 (NFE2L1 Human)
UseLentiviralTagsFLAG and HAExpressionMammalianMutationPromoterSFFVAvailable sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAB1619 hU6-BpiI-Cas13bt1-DR
Plasmid#176314PurposehU6-BpiI-Cas13bt1-DR (crRNA expression)DepositorInsertCas13bt1 crRNA direct repeat
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMN-332
Plasmid#195466PurposeLentiviral Integratable Inducible synZiFTR ZF1 + ReporterDepositorInsertinverted[8X ZF1 BS - ybTATA - mCherry] - pSFFV - ABI1 - ZF1 - 2A - p65 - PYL1
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAVS1 SA-2A-puro-pA donor
Plasmid#22075PurposeAAV zinc finger donor vector for genomic targetingDepositorInsertSA-Puro
UseZfn donorTagsExpressionMutationPromoterAvailable sinceOct. 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/opto-a1AR-EYFP
Plasmid#20947PurposeMammalian expression of opto-a1AR fused to EYFP for optical control of GPCR signalingDepositorInsertopto-a1AR
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
C1A-C1A-EYFP
Plasmid#61155PurposeDiacylglycerol (DAG) reporterDepositorInsertC1A-C1A-EYFP, C1A from PKCG (aa 26-89, rat) (Prkcg Rat)
UseTagsEYFPExpressionMammalianMutationC1A from PKCG amino acids 26-89PromoterCMVAvailable sinceDec. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
Cas9 TLS2
Plasmid#196978PurposeExpresses Cas9 fused with TLS2 mobility signal that confers graft mobility. Induced by estradiol.DepositorInsertCas9 with TLS2 tag
UseTagsExpressionPlantMutationPromoterpG10-90Available sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR-Tre3G-47xCAG-12xMS2
Plasmid#99148PurposeEncodes 47xCAG repeats with 12xMS2 tag, 2nd generation lentiviral vectorDepositorInsert47xCAG 12xMS2 hairpins
UseLentiviralTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTol2 hsp70l loxP-DsRed-loxP-nCas9n-GFP
Plasmid#158962PurposeCre-dependet Cas9-GFP expressionDepositorInsertshsp70l promoter
floxed DsRed cassette
Cas9-GFP
UseCRISPR and Cre/LoxTagsExpressionMutationPromoterAvailable sinceFeb. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
PL-SIN-Oct4-EGFP
Plasmid#21319PurposeLentiviral vector, expresses EGFP under Oct3/4 promoterDepositorInsertEnhanced Green Fluorescence Protein
UseLentiviralTagsExpressionMammalianMutationPromoterOct-4 promoter regionAvailable sinceJune 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
AAV-GFAP(long)-FLEX-GCaMP7f
Plasmid#189612PurposeCre-dependent GCaMP7f under the GFAP promoterDepositorInsertGCaMP7f
UseAAV and Cre/LoxTagsGCaMP7fExpressionMammalianMutationPromoterAvailable sinceSept. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA4-Beclin1 1-150
Plasmid#24389DepositorInsertBeclin1 (BECN1 Human)
UseTagsFLAGExpressionMammalianMutationContains amino acids 1-150.PromoterAvailable sinceApril 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAAV-synapsin-HaloCaMP1b-EGFP
Plasmid#138328Purposechemigenetic calcium indicatorDepositorHas ServiceAAV1 and AAV9InsertHaloCaMP1b
UseAAVTagsEGFP, His-tag, and Nuclear Exclusion SignalExpressionMammalianMutationPromotersynapsinAvailable sinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pL-CRISPR.SFFV.tRFP
Plasmid#57826PurposeLentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses tagRFP via P2A cleavage site. SFFV Promoter drivenDepositorInsertsSpCas9
Sp sgRNA scaffold
SFFV
P2A-tRFP
UseCRISPR and LentiviralTagsFLAGExpressionMutationPromoterAvailable sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-FB-dCas9-puro
Plasmid#100547PurposeExpresses N-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9 proteinDepositorInsertsN-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9
puromycin
UseCRISPRTagsFLAG, Biotin-acceptor-site (Avitag)ExpressionMammalianMutationPromoterHuman EF1aAvailable sinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pR26
Plasmid#127372PurposeAllows for doxycycline-inducible gene expression from the murine ROSA26 safe harbor locus upon CRISPR/Cas9-mediated genomic insertion and stable selection.DepositorTypeEmpty backboneUseCRISPR and Mouse TargetingTagsExpressionMutationPromotertight TRE promoterAvailable sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJG100
Plasmid#138966PurposePeft-3::wrmScarlet1-10::unc-54 3’UTR. Expresses wrmScarlet1-10 in somatic tissues of C. elegansDepositorInsertwrmScarlet1-10
UseTagsExpressionWormMutationPromoterAvailable sinceJuly 7, 2020AvailabilityAcademic Institutions and Nonprofits only