Addgene

Showing: 6521 - 6540 of 148587 results

Results per page:

Sort results by:

pABE8e-protein

Plasmid

#161788

Purpose

Rhamnose-inducible expression of 8xHIS-ABE8e in E. coli

Depositor

David Liu

Article

Huang et al Nat Protoc. 2021 Feb;16(2):1089-1128. do

Insert

ABE8e base editor

Use

Tags

8xHIS and BPNLS

Expression

Bacterial

Mutation

Promoter

pRhaBAD

Available since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
EF.STAT3DN.Ubc.GFP

Plasmid

#24984

Purpose

Depositor

Linzhao Cheng

Article

Hillion et al Cancer Research 68, 10121, December 2008

Insert

STAT3 (STAT3 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Y705F--dominant negative

Promoter

Available since

June 14, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
CAG-GFP

Plasmid

#16664

Depositor

Fred Gage

Article

Zhao et al J Neurosci. 2006 Jan 4. 26(1):3-11.

Insert

eGFP

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 2, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
Shp2 biosensor

Plasmid

#134346

Purpose

FRET biosensor. To visualize Shp2 activity by FRET

Depositor

Yingxiao Wang

Article

Sun et al Nat Commun. 2017 Sep 7;8(1):477. doi: 10

Insert

Shp2 Biosensor

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 22, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAVS1-P-CAG-mCh

Plasmid

#80492

Purpose

donor vector for AAVS1 targeting (puromycin selection) and constitutive mCherry expression

Depositor

Knut Woltjen

Article

Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

Insert

mCherry

Use

Donor vector (human)

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pKLV2-U6gRNA5(Empty)-PGKBFP2AGFP-W

Plasmid

#67979

Purpose

Cas9 activity reporter (control) with BFP and GFP.

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKBFP2AGFP cassette, WPRE

Use

Lentiviral

Tags

Expression

Mutation

Deleted BbsI site within WPRE

Promoter

human U6 promoter, mouse PGK promoter

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP2-NbALFA

Plasmid

#201486

Purpose

BRET2 detection of ALFA-tagged protein

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

GFP2-NbALFA

Use

Tags

GFP2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
MAVS-WT

Plasmid

#52135

Purpose

Retroviral vector for expression of human MAVS in mammalian cells

Depositor

Jonathan Kagan

Article

Dixit et al Cell. 2010 May 14;141(4):668-81. Epub 20

Insert

MAVS (MAVS Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pThy1-Brainbow3.2

Plasmid

#45179

Depositor

Joshua Sanes

Article

Cai et al Nat Methods. 2013 May 5;10(6):540-7. doi

Insert

pThy1-Brainbow3.2

Use

Linearized for transgenic mouse injection

Tags

Expression

Mutation

Promoter

Thy1

Available since

Nov. 19, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
Sema3a(L, del3)-Fc-His

Plasmid

#72136

Purpose

Expresses the Sema3A protein (truncated at cleavage site P3; ie, long and missing exon 3), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3a (Sema3a Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pAM-AAV-mSncg-Cre

Plasmid

#153207

Purpose

Mouse gamma-synuclein (mSncg) promoter-mediates Cre expression in retinal ganglion cells

Depositor

Yang Hu

Article

Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

Insert

Cre

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

mSncg

Available since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSET FLIPE intramol-1u

Plasmid

#13549

Depositor

Wolf Frommer

Article

Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

Insert

FLIP-E intramol WT (gltI E. coli)

Use

Tags

EYFP

Expression

Bacterial

Mutation

ECFP insert in binding protein

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pMAM-RecG-ST2TaV-EGFP

Plasmid

#31275

Depositor

Andrew Pierce

Article

Killen et al BMC Mol Biol. 2012 Oct 30;13:33. doi: 10

Insert

RecG-ST2-TaV-EGFP

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pME-24xMBS

Plasmid

#86246

Purpose

Middle entry vector for generating MBS tagged RNAs

Depositor

Florence Marlow

Article

Campbell et al Development. 2015 Apr 1;142(7):1368-74.

Type

Empty backbone

Use

Unspecified

Tags

NLS-tdMCP

Expression

Mutation

Promoter

Available since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV-EFS-SaABE8e-bGH-U6-sgRNA-BsmBI

Plasmid

#189922

Purpose

AAV genome encoding SaABE8e and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installation

Depositor

David Liu

Article

Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

Insert

SaABE8e

Use

AAV

Tags

Expression

Mutation

SaCas9 D10A

Promoter

EFS

Available since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
nls-EGFP

Plasmid

#67652

Purpose

Mammalian expression vector driving nls-EGFP.

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

nls-EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-GRK3

Plasmid

#32689

Depositor

Robert Lefkowitz

Article

Pitcher et al J Biol Chem. 1996 Oct 4. 271(40):24907-1

Insert

GRK3 (GRK3 Bovine)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 21, 2012

Availability

Academic Institutions and Nonprofits only
mApple-LAMP1-phLuorin-N-8

Plasmid

#54918

Purpose

Localization: Lysosome Membrane, Excitation: 568, Emission: 592

Depositor

Michael Davidson

Insert

LAMP1 phLuorin

Use

Tags

mApple

Expression

Mammalian

Mutation

Lysosomes-20 double labeled with phLuorin

Promoter

CMV

Available since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pET22b_PstS_3

Plasmid

#78200

Purpose

Expression of double-cysteine variant of mature phosphate binding protein, PstS, which also has a mutation to weaken phosphate binding, as scaffold for phosphate biosensor

Depositor

Martin Webb

Article

Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62

Insert

pstS (A17C,I76G,A197C)

Use

Tags

Expression

Bacterial

Mutation

A17C,I76G,A197C (please see depositor comment bel…

Promoter

T7

Available since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV sgRNA Expression Plasmid

Plasmid

#174540

Purpose

Contains restriction sites to clone in U6 promoter and sgRNA oligo. CMV-driven mCherry fluorescent marker.

Depositor

Ormond MacDougald

Article

Romanelli et al J Biol Chem. 2021 Nov 11:101402. doi: 10

Type

Empty backbone

Use

AAV

Tags

None

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge