Skip to main content
Addgene
Showing: 3261 - 3270 of 8667 results
  1. pDule2-3-nitroTyrosine (5B)

    Plasmid
    #85499
    Purpose
    Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the Mj 3NY (5B) synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Insert
    3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Y32H H70C D158S I159A L162R
    Promoter
    lpp (constitutive)
    Available since
    Jan. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
  2. scFv-GCN4-DNMT3a-DNMT3l

    Plasmid
    #154140
    Purpose
    Expresses the scFv-GCN4-DNMT3a-DNMT3l fusion protein (more details are shown in the vectro map) for targeted DNA methylation. Should be used in a combination with the dCas9-SunTag systems.
    Depositor
    Insert
    scFv-GCN4, DNMT3a (catalytic domain), DNMT3l (C-terminal part), sfGFP
    Use
    Tags
    HA and sfGFP
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV
    Available since
    Oct. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
  3. pYPQ269E2

    Plasmid
    #164721
    Purpose
    Gateway entry clone (attL1 & attR5) A3A/Y130F-CBE_V04 for C-T base editing
    Depositor
    Insert
    A3A(Y130F)-zCas9(D10A)-UGI-T2A-MS2-UGI
    Use
    CRISPR; Gateway compatible a3a/y130f-cbe_v04 entr…
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    May 21, 2021
    Availability
    Academic Institutions and Nonprofits only
  4. 139H2_LC

    Plasmid
    #206202
    Purpose
    For recombinant expression of the light chain of the Mouse anti-MUC1 antibody 139H2 in mammalian cells.
    Depositor
    Insert
    anti-MUC1 antibody 139H2 light chain (Igkc Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
Showing: 3261 - 3270 of 8667 results