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pSpCas9(BB)-2A-GFP_MAPRE1-KI-gRNA#1

Plasmid

#197424

Purpose

π-element knock-in into exon 5 of MAPRE1

Depositor

Torsten Wittmann

Article

Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

Insert

MAPRE1 gRNA #1 (targets Exon 5) for π-element knock-in (MAPRE1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pSpCas9(BB)-2A-GFP_MAPRE1-KI-gRNA#2

Plasmid

#197425

Purpose

π-element knock-in into exon 5 of MAPRE1

Depositor

Torsten Wittmann

Article

Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

Insert

MAPRE1 gRNA #2 (targets Exon 5) for π-element knock-in (MAPRE1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pSpCas9(BB)-2A-GFP_MAPRE1-KI-gRNA#3

Plasmid

#197426

Purpose

π-element knock-in into exon 5 of MAPRE1

Depositor

Torsten Wittmann

Article

Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

Insert

MAPRE1 gRNA #3 (targets Exon 5) for π-element knock-in (MAPRE1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC19-π-EB1_EF1α-EGFP

Plasmid

#197430

Purpose

Homology-directed repair template targeting EF1α-based π-element to MAPRE1 exon 5

Depositor

Torsten Wittmann

Article

Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

Insert

EF1α-based π-element with HDR templates flanking MAPRE1 exon 5 insertion site (MAPRE1 Human)

Use

CRISPR and Synthetic Biology

Tags

EGFP, GCN4 leucine zipper, LOV2, and Zdk1

Expression

Mutation

Promoter

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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PGK-EGFP-FKBPF36V

Plasmid

#199765

Purpose

plasmid for targeting dTAG into the AAVS1 locus

Depositor

Hannes Lans

Article

van der Woude et al Nucleic Acids Res. 2023 Oct 13;51(18):e9

Insert

GFP-dTAG

Use

Tags

GFP and dTAG

Expression

Mammalian and Worm

Mutation

Promoter

hPGK

Available since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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Dcp2 H237A-EGFP

Plasmid

#201425

Purpose

Bacterial protein expression of Dcp2 H237A

Depositor

Michael Rosen

Article

Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Dcp2 (DCP2 Budding Yeast)

Use

Tags

EGFP, TEV, 6xHis and MBP, TEV

Expression

Bacterial

Mutation

H237A

Promoter

tac

Available since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
MCS:GFPnls-T2a-ER-Cre-ER

Plasmid

#200106

Purpose

Vector for LFNG

Depositor

Prayag Murawala

Type

Empty backbone

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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LFNG:GFPnls-T2a-ER-Cre-ER

Plasmid

#200107

Depositor

Prayag Murawala

Insert

LFNG:GFPnls-T2A-ER-Cre-ER

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Dhh1 25-425-EGFP

Plasmid

#201430

Purpose

Bacterial protein expression of Dhh1 25-425

Depositor

Michael Rosen

Article

Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Dhh1 (DHH1 Budding Yeast)

Use

Tags

EGFP, TEV, 6xHis and MBP, TEV

Expression

Bacterial

Mutation

aa 25-425 only

Promoter

tac

Available since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Dcp1 H206A-EGFP

Plasmid

#201424

Purpose

Bacterial protein expression of Dcp1 H206A

Depositor

Michael Rosen

Article

Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Dcp1 (DCP1 Budding Yeast)

Use

Tags

EGFP, TEV, 6xHis and MBP, TEV

Expression

Bacterial

Mutation

H206A

Promoter

tac

Available since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Dcp1 82-129-EGFP

Plasmid

#201427

Purpose

Bacterial protein expression of Dcp1 82-129

Depositor

Michael Rosen

Article

Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Dcp1 (DCP1 Budding Yeast)

Use

Tags

EGFP, TEV, 6xHis and MBP, TEV

Expression

Bacterial

Mutation

aa 82-129 only

Promoter

tac

Available since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Pat1 1-240-EGFP

Plasmid

#201435

Purpose

Bacterial protein expression of Pat1 1-240

Depositor

Michael Rosen

Article

Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Pat1 (PAT1 Budding Yeast)

Use

Tags

EGFP, TEV, 6xHis and MBP, TEV

Expression

Bacterial

Mutation

aa 1-240 only

Promoter

tac

Available since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Edc3 67-282-EGFP

Plasmid

#201433

Purpose

Bacterial protein expression of Edc3 67-282

Depositor

Michael Rosen

Article

Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Edc3 (EDC3 Budding Yeast)

Use

Tags

EGFP, TEV, 6xHis and MBP, TEV

Expression

Bacterial

Mutation

aa 67-282 only

Promoter

tac

Available since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Dcp1 H198A-EGFP

Plasmid

#201423

Purpose

Bacterial protein expression of Dcp1 H198A

Depositor

Michael Rosen

Article

Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Dcp1 (DCP1 Budding Yeast)

Use

Tags

EGFP, TEV, 6xHis and MBP, TEV

Expression

Bacterial

Mutation

H198A

Promoter

tac

Available since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Dcp1 H43A-EGFP

Plasmid

#201422

Purpose

Bacterial protein expression of Dcp1 H43A

Depositor

Michael Rosen

Article

Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Dcp1 (DCP1 Budding Yeast)

Use

Tags

EGFP, TEV, 6xHis and MBP, TEV

Expression

Bacterial

Mutation

H43A

Promoter

tac

Available since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Dcp1 H40A-EGFP

Plasmid

#201421

Purpose

Bacterial protein expression of Dcp1 H40A

Depositor

Michael Rosen

Article

Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Dcp1 (DCP1 Budding Yeast)

Use

Tags

EGFP, TEV, 6xHis and MBP, TEV

Expression

Bacterial

Mutation

H40A

Promoter

tac

Available since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Dcp2 1-300-EGFP

Plasmid

#201428

Purpose

Bacterial protein expression of Dcp2 1-300

Depositor

Michael Rosen

Article

Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Dcp2 (DCP2 Budding Yeast)

Use

Tags

EGFP, TEV, 6xHis and MBP, TEV

Expression

Bacterial

Mutation

aa 1-300 only

Promoter

tac

Available since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pT2/sh-Col1a1/GFP4_Seq1.2

Plasmid

#201403

Purpose

Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

Depositor

Maria Castro

Article

Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

Insert

COL1A1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pT2/sh-FYN1-GFP_Seq_C

Plasmid

#201399

Purpose

Knockdown of FYN tyrosine kinase. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

Depositor

Maria Castro

Article

Comba et al Neuro Oncol. 2020 Jun 9;22(6):806-818. d

Insert

FYN

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pFB-CG-LYCHOS WT-HA-GFP

Plasmid

#199687

Purpose

Expression of LYCHOS WT in insect cells

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

LYCHOS (GPR155 Human)

Use

Tags

10xHis, EGFP, HA, HRV 3C, and His

Expression

Insect

Mutation

Promoter

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge