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Plasmid#40260PurposeRFP with robust performance in protein fusions and useful as FRET acceptor for Clover in a FRET pair that offers bright fluorescence, dynamic range, and photostability while limiting emissions overlapDepositorHas ServiceCloning Grade DNAInsertmRuby2
UseTagsHis6 and XpressExpressionMammalianMutationPromoterCMV IEAvailable sinceSept. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSBtet-GP
Plasmid#60495PurposeSB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of GFP, rtTA and puromycin resistance geneDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTransposonTagsExpressionMammalianMutationPromoterTCE & RPBSAAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLminP_Luc2P_RE10
Plasmid#90344PurposeIRF3 - Viral response gene reporterDepositorInsertFirefly luciferase
UseLentiviralTagsExpressionMutationPromoterIRF3Available sinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHIV-dTomato
Plasmid#21374Purposeself-inactivating lentiviral plasmid for co-expression of your gene of interest and dTomatoDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 19, 2009AvailabilityAcademic Institutions and Nonprofits only -
pMJ114
Plasmid#85995PurposeOne-guide Perturb-seq vector backbone; modified bovine U6 promoter; original constant regionDepositorInsertssgGFP-NT2
puro-T2A-BFP
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermodified bovine U6-2Available sinceFeb. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKM461
Plasmid#108320PurposeFor performing ORBIT - Expresses Che9c phage RecT and Bxb1 phage Integrase, and contains SacBDepositorInsertsChe9C RecT
Bxb1 Integrase
Tet Repressor
SacRB
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
lentiGuide-Hygro-mTagBFP2
Plasmid#99374PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A mTagBFP2 from EF-1a promoter. 3rd generation lentiviral backbone.DepositorInsertsS. pyogenes sgRNA cassette
Hygro-P2A-mTagBFP2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF-1a and hU6Available sinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA_RFP-TEVprotease
Plasmid#124947PurposeExpression of RFP-TEVproteaseDepositorInsertRFP-TEVprotease
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 mCherry
Plasmid#128073Purposepuro exchanged to mCherryDepositorInsertmCherry
UseLentiviralTagsExpressionMammalianMutationnoPromoterAvailable sinceAug. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-UbC-FKRP-EF1a-BSD
Plasmid#205150PurposeExpress UbC-driven FKRP and EF-1a driven BSDDepositorInsertFukutin-related protein (FKRP Human)
UseLentiviralTagsExpressionMammalianMutationPromoterUbCAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-rtTA-IRES-Hygro
Plasmid#102423PurposeMammalian expression vector for tetracycline-transactivator (rtTA), IRES-HygroDepositorInserttetracyclin-transactivator (rtTA)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceNov. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
Snap-Omp25
Plasmid#69599PurposeSnap-tag substrates with two different fluorophores (red and green) sequentially to separately label young and old mitochondriaDepositorInsertSnap-Omp25
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pQC membrane TdTomato IX
Plasmid#37351DepositorInsertmembrane TdTomato
UseTagsPalmitoylation signal*ExpressionMutationPromoterAvailable sinceJuly 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
AA028
Plasmid#216008PurposeFragmid fragment: (Cas protein) nickase Cas for base editingDepositorHas ServiceCloning Grade DNAInsertnCas9 (D10A)_v1.1 [Sp]
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA029
Plasmid#216009PurposeFragmid fragment: (Cas protein) nickase Cas for base editingDepositorHas ServiceCloning Grade DNAInsertnCas9-NG (D10A)_v1.1 [Sp]
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
mEGFP
Plasmid#18696DepositorInsertmonomeric EGFP
UseTagsExpressionMammalianMutationA206K mutationPromoterAvailable sinceJuly 2, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBABE-neo-hTERT
Plasmid#1774PurposeRetroviral expression of hTERT. Used to create immortalized cell lines.DepositorInserthTERT (TERT Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 26, 2005AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) Drp1
Plasmid#34706DepositorInsertDrp1 (Dnm1l Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-eNpHR 3.0-EYFP
Plasmid#26972PurposeAAV expression of eNpHR 3.0 fused to EYFP driven by humanized Synapsin promoter for optogenetic inhibitionDepositorHas ServiceAAV5InserteNpHR 3.0
UseAAVTagsEYFPExpressionMammalianMutationTrafficking Signal (TS) ER Export SignalPromoterhSynAvailable sinceFeb. 4, 2011AvailabilityAcademic Institutions and Nonprofits only