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pcDNA3-HA-DNM2-W525L

Plasmid

#155263

Purpose

Mammalian expression of rat DNM2 fused to HA

Depositor

David Rubinsztein

Article

Puri et al Dev Cell. 2020 Apr 20;53(2):154-168.e6.

Insert

dynamin 2 (Dnm2 Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

Tryptophan 525 to Leucine

Promoter

Available since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-DNM2-R465W

Plasmid

#155262

Purpose

Mammalian expression of rat DNM2 fused to HA

Depositor

David Rubinsztein

Article

Puri et al Dev Cell. 2020 Apr 20;53(2):154-168.e6.

Insert

dynamin 2 (Dnm2 Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

Arginine 465 to Tryptophan

Promoter

Available since

May 14, 2021

Availability

Academic Institutions and Nonprofits only

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pG418R-MCS-QF-MiniWhite

Plasmid

#165902

Purpose

G418 resistant QF driver vector with Mini-white CDS eye marker. Contains an enhancer grammar GB20 entry point for custom enhancers. Standard cut-and-paste cloning can also be used. Vector uses Purple-White bacteria colony screening for identification of correct cloning

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Selectable Empty Enhancer Driver

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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mILK-L207W-EFGP

Plasmid

#176900

Purpose

mouse ILK with L207W point mutation cloned into pEGFP-N1

Depositor

Sara Wickström

Article

Martin et al Proc Natl Acad Sci U S A. 2022 Mar 15;11

Insert

Integrin-linked kinase (Ilk Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Leucine 207 to Tryptophane

Promoter

CNV

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pDual-eGFP(W66)

Plasmid

#63217

Purpose

Expression of the Celeste (cyan) spectral variant of eGFP in bacteria and in mammalian cells. Could be used as a reporter for quantifying gene targeting and recombineering in mammalian cells.

Depositor

Richard S Myers

Article

Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1

Insert

His-T7-eGFP(W66)

Use

Lentiviral

Tags

His6 and T7

Expression

Bacterial and Mammalian

Mutation

Changed tyrosine at position 66 with respect to t…

Promoter

CMV-EF1α hybrid (CEF)

Available since

April 15, 2015

Availability

Academic Institutions and Nonprofits only
HBM-mVEGF-C-HisTag-pENTR

Plasmid

#64066

Purpose

Plasmid encodes mature form of murine VEGF-C preceded by honey bee melittin secretion signal; entry vector to Gateway cloning system.

Depositor

Joanna Bereta

Article

Klaus et al Protein Expr Purif. 2015 Mar 7. pii: S10

Insert

vascular endothelial growth factor C (Vegfc Mouse)

Use

Tags

His-tag

Expression

Insect

Mutation

Promoter

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pYPQ134-STU-Fn

Plasmid

#138104

Purpose

Golden Gate entry vector for 4th crRNA cloning with FnCas12a crRNA scaffold flanked by HH and HDV ribozymes

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ131-STU-Fn

Plasmid

#138095

Purpose

Golden Gate entry vector for 1st crRNA cloning with FnCas12a crRNA scaffold flanked by HH and HDV ribozymes

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ132-STU-Fn

Plasmid

#138098

Purpose

Golden Gate entry vector for 2nd crRNA cloning with FnCas12a crRNA scaffold flanked by HH and HDV ribozymes

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ133-STU-Fn

Plasmid

#138101

Purpose

Golden Gate entry vector for 3rd crRNA cloning with FnCas12a crRNA scaffold flanked by HH and HDV ribozymes

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR-mDIXDC1 WT

Plasmid

#61216

Purpose

Entry clone encoding long isoform of mouse DIXDC1

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

DIXDC1 (Dixdc1 Mouse)

Use

Donr clone (gateway)

Tags

Expression

Mutation

Promoter

Available since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only
pCR8/GW/TOPO-TBX20a

Plasmid

#98620

Purpose

Gateway entry TOPO TA cloning vector for TBX20a

Depositor

Richard Harvey

Article

Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

Insert

Tbx20a (Tbx20 Mouse)

Use

Topo ta cloning vector

Tags

Expression

Mutation

Promoter

Available since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC39A5

Plasmid

#132057

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Polesel et al Biometals. 2022 Dec 1. doi: 10.1007/s105

Insert

SLC39A5 (SLC39A5 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 8, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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hFlt1 V767A

Plasmid

#86038

Purpose

human VEGFR1 with V to A mutation at position 767

Depositor

Christie Thomas

Article

Raikwar et al Exp Cell Res. 2016 May 15;344(1):103-11.

Insert

human Flt1 V767A (FLT1 Human)

Use

Tags

FLAG, HA (please see depositor comments below), a…

Expression

Mammalian

Mutation

V767A

Promoter

CMV

Available since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pFastBac1-OSF-TRIMCyp (owl monkey)(K283D, Q287D)

Plasmid

#79036

Purpose

Baculoviral entry vector to produce Bac-to-bac baculoviruses

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

OSF-TRIM5Cyp (owl monkey) (K283D, Q287D)

Use

Tags

OneSTrEP-FLAG

Expression

Insect

Mutation

Changed Lys 283 to Asp and Gln 287 to Asp

Promoter

polyhedrin

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pGG-PIP53

Plasmid

#196791

Purpose

Contains EDS16 promoter

Depositor

Marc Somssich

Article

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

ENHANCED DISEASE SUSCEPTIBILITY TO ERYSIPHE ORONTII 16 (EDS16 Mustard Weed)

Use

Greengate (gg) entry vector

Tags

Expression

Mutation

1579 G->C ; 1049 C->G

Promoter

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGG-PIP06

Plasmid

#196744

Purpose

Contains BAK1 promoter

Depositor

Marc Somssich

Article

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

BRI1-ASSOCIATED RECEPTOR KINASE 1 (BAK1 Mustard Weed)

Use

Greengate (gg) entry vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGG-PIP07

Plasmid

#196745

Purpose

Contains CERK1 promoter

Depositor

Marc Somssich

Article

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

CHITIN ELICITOR RECEPTOR KINASE 1 (CERK1 Mustard Weed)

Use

Greengate (gg) entry vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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p5E-EFS (EF1a core) (JDW 1319)

Plasmid

#224474

Purpose

Gateway compatible 5' entry clone with human alpha 2 globin pause site (to prevent transcription readthrough) and EF1a core promoter. Lacks downstream intron for a lower-activity, compact promoter.

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

EFS promoter

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pME-Luciferase-P2A-H2A-mCherry (JDW 926)

Plasmid

#224488

Purpose

Gateway compatible middle entry clone containing a Luciferase P2A H2A-mCherry dual reporter (Luciferase/nuclear red fluorescent reporter)

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

Luciferase-P2A-H2A-mCherry

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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