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Plasmid#159999PurposeExpresses SARS-CoV-2 RBD (340-538) (receptor-binding domain from Spike) with N-terminal SpyTag fusion in mammalian cellsDepositorInsertSpyTag-SARS-CoV-2 RBD (340-538) (S SARS-CoV-2)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCR8/GW_TOPO_Cdk12_Untagged
Plasmid#127176PurposeUntagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into Gateway entry vectorDepositorInsertMouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)
UseEntry vector with transgene for gateway cloningTagsNoneExpressionMutationPromoterNoneAvailable sinceJuly 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT 3xFLAG-KPNB1
Plasmid#192301PurposeGateway entry vector for an inducible 3xFLAG-KPNB1DepositorInsertKPNB1 (KPNB1 Human)
UseGateway entry vectorTags3xFLAGExpressionMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A mKate2-hu_ncOGT K842M
Plasmid#154284PurposeEntry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; K842M variant, catalytic dead)DepositorInsertO-GlcNAc Transferase (OGT Human)
UseGateway entry vectorTags2x FLAG and mKate2ExpressionMutationK842M mutation (Catalytic Inactive)PromoterAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-FGFR4
Plasmid#60531PurposeEntry vector for human FGFR4DepositorInsertFGFR4 (FGFR4 Human)
UseEntry vectorTagsExpressionMutationc2134a P712TPromoterNAAvailable sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNSSCP
Plasmid#183188PurposePACE-evolved split N (core) terminal of T7 RNA polymerase-SpmXΔC and SpmXΔC-PACE-evolved split C (sigma) terminal of T7 RNA polymerase both expressed under Tac promoter; DivIVA-sfGFP expressed under TDepositorInsertsDivIVA and superfolder GFP fusion protein
SpmX450 and T7 181+ fusion protein
T7 RNAP 1-179 variant and SpmX450 fusion protein
mRFP1 and popZ fusion protein
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
FUG-T2A-Pten
Plasmid#140738PurposeLentiviral vector to overexpress human PTEN and GFPDepositorInsertGFP-PTA-PTEN (PTEN Human)
UseLentiviralTagsnoneExpressionMammalianMutationPromoterhUbiCAvailable sinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
Flag-betaPIXa
Plasmid#15234DepositorInsertbeta-PIXa (Arhgef7 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceSept. 13, 2007AvailabilityAcademic Institutions and Nonprofits only -
SLK_HUMAN_D0
Plasmid#79733PurposeThis plasmid encodes the kinase domain of SLK. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertSLK (SLK Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A mKate2-hu_ncOGT D554N
Plasmid#154283PurposeEntry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; D554N variant, no Ser/Thr glycosylation)DepositorInsertO-GlcNAc Transferase (OGT Human)
UseGateway entry vectorTags2x FLAG and mKate2ExpressionMutationD554N mutation (Ser/Thr Glycosylation Impaired)PromoterAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only