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pZKSCAN4.1.0-gDNA

Plasmid

#112465

Purpose

CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZKSCAN4

Depositor

Kevin White

Insert

ZKSCAN4 (ZKSCAN4 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pNR2F6.1.0-gDNA

Plasmid

#112456

Purpose

CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor NR2F6

Depositor

Kevin White

Insert

NR2F6 (NR2F6 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pZNF197.1.0-gDNA

Plasmid

#112453

Purpose

CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZNF197

Depositor

Kevin White

Insert

ZNF197 (ZNF197 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pMYNN.1.0-gDNA

Plasmid

#112450

Purpose

CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor MYNN

Depositor

Kevin White

Insert

MYNN (MYNN Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pZNF639.1.0-gDNA

Plasmid

#112441

Purpose

CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZNF639

Depositor

Kevin White

Insert

ZNF639 (ZNF639 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pZNF212.1.0-gDNA

Plasmid

#112427

Purpose

CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZNF212

Depositor

Kevin White

Insert

ZNF212 (ZNF212 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pZNF23.1.0-gDNA

Plasmid

#112421

Purpose

CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZNF23

Depositor

Kevin White

Insert

ZNF23 (ZNF23 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pZNF134.1.0-gDNA

Plasmid

#112416

Purpose

CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZNF134

Depositor

Kevin White

Insert

ZNF134 (ZNF134 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pNR1D2.1.0-gDNA

Plasmid

#112410

Purpose

CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor NR1D2

Depositor

Kevin White

Insert

NR1D2 (NR1D2 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pZNF354B.1.0-gDNA

Plasmid

#112405

Purpose

CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZNF354B

Depositor

Kevin White

Insert

ZNF354B (ZNF354B Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pZNF75A.1.0-gDNA

Plasmid

#112403

Purpose

CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZNF75A

Depositor

Kevin White

Insert

ZNF75A (ZNF75A Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

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PX458_HHEX_2

Plasmid

#72355

Purpose

Encodes gRNA for 3' target of human HHEX along with Cas9 with 2A GFP

Depositor

Richard M. Myers, Eric Mendenhall

Insert

HHEX (HHEX Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Feb. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pUCC001

Plasmid

#124451

Purpose

Multi-species CRISPR/Cas9 coexpression system, optimized for Golden Gate cloning of gRNA targets

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

HH-BsaI

Use

Tags

Expression

Yeast

Mutation

gRNA expression cassette from pUDP002 modified to…

Promoter

TDH3 promoter from S.cerevisiae (already present …

Available since

Sept. 26, 2019

Availability

Academic Institutions and Nonprofits only

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sgCtr- LentiCRISPRv2

Plasmid

#107402

Purpose

Lentiviral expression of Cas9 and a control gRNA

Depositor

William Kaelin

Article

Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

Insert

control gRNA

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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DR274

Plasmid

#42250

Purpose

guide RNA (gRNA) expression vector used to create a gRNA to a specific sequence; uses T7 promoter for in vitro transcription

Depositor

Keith Joung

Article

Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR; Zebrafish expression

Tags

Expression

Worm

Mutation

Promoter

T7

Available since

Jan. 31, 2013

Availability

Academic Institutions and Nonprofits only

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pAGM4723:TpCC_Urease

Plasmid

#85982

Purpose

Golden Gate Level 2 construct encoding Cas9-YFP and 2 urease-targeting gRNAs

Depositor

Thomas Mock

Article

Hopes et al Plant Methods. 2016 Nov 24;12:49. doi: 1

Inserts

Cas9
Urease-targeting gRNA 1
Urease-targeting gRNA 2

Use

CRISPR; Diatom (t. pseudonana)

Tags

NLS and YFP

Expression

Mutation

Promoter

FCP and U6

Available since

Jan. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pDECKO_GFP

Plasmid

#72619

Purpose

Expresses two gRNAs targeting GFP

Depositor

Rory Johnson, Roderic Guigo

Article

Aparicio-Prat et al BMC Genomics. 2015 Oct 23;16(1):846. doi

Insert

gRNAs toward GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 (gRNA1) and H1 (gRNA2)

Available since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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PX458_TEAD1_1

Plasmid

#86356

Purpose

Encodes gRNA for 3' target of human TEAD1

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against TEAD1 (TEAD1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pMSCV-BLU-VIPR

Plasmid

#220497

Purpose

Retroviral plasmid allowing for potent induction of gRNA using blue light. The plasmid contains VPR-EL222-2A-Thy1.1 and the C120 element followed by cloning sites for ribozyme flanked gRNA (RGR).

Depositor

Alexander Espinosa

Article

Pulgarin et al bioRxiv [Preprint]. 2023 Nov 10:2023.11.

Type

Empty backbone

Use

CRISPR and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pBLU-VIPR

Plasmid

#220498

Purpose

Plasmid allowing for potent induction of gRNA using blue light. The plasmid contains VPR-EL222 and the C120 response element followed by mCherry and cloning sites for ribozyme flanked gRNA (RGR).

Depositor

Alexander Espinosa

Article

Pulgarin et al bioRxiv [Preprint]. 2023 Nov 10:2023.11.

Type

Empty backbone

Use

CRISPR; Transposon

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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