Addgene

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AsCas12a-DR-cr in PBCSK-

Plasmid

#126640

Purpose

Cloning vector AsCas12a DR-crRNA for expression in mammalian cells

Depositor

Scot Wolfe

Article

Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

Insert

AsCas12a Full Length Direct Repeat crRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pSR241

Plasmid

#125091

Purpose

Expression of ompR(1-137aa)-yxdJ(132-229aa) chimera under PLtetO-1, output Promoter PyxdL181

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

ompR(REC)-yxdJ(DBD)137
sfgfp
tetR

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pGWB418

Plasmid

#74812

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with 4xMyc (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

Sept. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB519

Plasmid

#74861

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with 10xMyc (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pINDgw RFA-V5-EcoDam

Plasmid

#59204

Purpose

Mammalian DamID vector for fusing gene of interest with Dam-V5 using Gateway cloning

Depositor

Bas van Steensel

Article

Vogel et al Genome Res. 2006 Dec;16(12):1493-504. Ep

Type

Empty backbone

Use

Damid

Tags

Dam (DNA adenine methyltransferase) and V5

Expression

Mammalian

Mutation

Promoter

Heat Shock Minimal Promoter

Available since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB551

Plasmid

#74881

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with G3GFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pHS1068

Plasmid

#205962

Purpose

NZ_CP025815 DinG HNH proteins (E. coli codon optimized) and CRISPR array in pACYCDuet-1 with Lac promoters

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

DinG HNH proteins and CRISPR array

Use

Tags

Expression

Bacterial

Mutation

Promoter

Lac (proteins), pJ23119 (crRNA)

Available since

Feb. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pGWB552

Plasmid

#74882

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with G3GFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB508

Plasmid

#74850

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with 6xHis (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

Oct. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB416

Plasmid

#74810

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with 4xMyc (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pBP-split-intein-Gal4[1-20]-N-int

Plasmid

#199201

Purpose

Gateway destination vector for enhancer-driven split-intein Gal4[1-20]N-int expression

Depositor

Norbert Perrimon

Article

Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

Insert

split-intein-Gal4[1-20]-N-int

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pBP-split-intein-Gal4[21-881]-C-int

Plasmid

#199202

Purpose

Gateway destination vector for enhancer-driven split-intein Gal4[21-881]C-int expression

Depositor

Norbert Perrimon

Article

Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

Insert

split-intein-Gal4[21-881]-C-int

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pGLTR-X-GFP

Plasmid

#58245

Purpose

GATEWAY-compatible lentiviral conditional RNAi delivery vector; SFFV-driven expression of TetR and eGFP

Depositor

Stephan Geley

Article

Sigl et al PLoS One. 2014 May 19;9(5):e97764. doi:

Inserts

attR1-Cm(R)-CcdB-attR2
TetR-NLS-T2A-GFP

Use

Lentiviral and RNAi; Gateway destionation vector

Tags

GFP, NLS, T2A, and TetR

Expression

Mammalian

Mutation

Promoter

SFFV and no

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pCD565

Plasmid

#153027

Purpose

dxCas9(3.7), MCP-SoxS(R93A/S101A), J306 scRNA

Depositor

Jesse Zalatan

Article

Fontana et al Nat Commun. 2020 Apr 1;11(1):1618. doi:

Insert

dxCas9(3.7), MCP-SoxS(R93A/S101A), J306 scRNA

Use

CRISPR

Tags

Expression

Bacterial

Mutation

SoxS has R93A and S101A mutations

Promoter

Available since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pGWB423

Plasmid

#74817

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with GST (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

July 14, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE463

Plasmid

#153234

Purpose

Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

Depositor

Sylvestre Marillonnet

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pGWB440

Plasmid

#74826

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with eYFP (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Sept. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB523

Plasmid

#74865

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with GST (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB507

Plasmid

#74849

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with 6xHis (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pEM-Cas9HF1-indRecA56

Plasmid

#102294

Purpose

Modified from pEM-Cas9HF1 (Addgene ID: 89961) to co-express inducible recA56 to block recA-mediated double-strand break repair.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Inserts

Cas9HF1
recA56

Use

CRISPR

Tags

Expression

Bacterial

Mutation

N497A/R661A/Q695A/Q926A

Promoter

pTet

Available since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

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