-
Plasmid#180786PurposeUnique Projection Barcode (UPB4)-Colon for Projection-seqDepositorInserthM3Dq ( partial )
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWZL hygro 12S E1A
Plasmid#18748DepositorInsert12S E1A
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
SG3ΔENV CA E45A
Plasmid#145783PurposeNon-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS Reagent Program Cat #11051) and E45A mutation in capsid (CA E45A).DepositorInsertCapsid
UseLentiviral and RetroviralTagsExpressionMammalianMutationChanged Capsid residue glutamic acid 45 to alanin…PromoterAvailable sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-15F11-HA-Halo
Plasmid#129592PurposeThe encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the HaloTag. It can be used to track mature and nascent HA tagged proteins in living organism.DepositorInsertAnti-HA frankenbody-HaloTag (15F11-HA scFv-HaloTag)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFRLuc-CoV2-WT
Plasmid#177614PurposeDual luciferase reporter for SARS-CoV-2 programmed -1 ribosomal frameshiftingDepositorInsertSARS-CoV-2 FSE
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
sgRNA 1 GAPDH
Plasmid#83808PurposeExpress Sg-1 sgRNA targeting GAPDHDepositorInsertSgRNA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
LeGO-dKatushka2
Plasmid#85214PurposeLentiviral vector expressing dKatushka2 fluorescent protein driven by SFFV promoterDepositorInsertdKatushka2
UseCre/Lox and LentiviralTagsExpressionMammalianMutationBsrGI site removed at 5' end, BsrGI site ins…PromoterSFFVAvailable sinceJan. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pErCas12a EZ Clone
Plasmid#132641PurposeExpresses ErCas12a and an sgRNA to target a region of interestDepositorInsertErCas12a
UseAAV and CRISPRTags3x HA and SV40 NLSExpressionMammalianMutationQ926R (Please see depositor comments) and BsaI si…PromoterCMV PromoterAvailable sinceApril 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET11d-hUba2
Plasmid#53136PurposeE.coli expression of full length untagged human Uba2 (SAE2)DepositorInsertUBA2 (UBA2 Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn1_AchLightG
Plasmid#187464PurposeExpresses green acetylcholine indicator AchLightG in neuronal cellsDepositorInsertAchLightG
UseAAVTagsIgκ leaderExpressionMutationPromoterhuman Synapsin-1Available sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-Atg5
Plasmid#24922DepositorInsertAtg5 (ATG5 Human)
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceJune 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Flag-Tet3
Plasmid#60940PurposeExpresses Tet3 in mammalian cellsDepositorInsertTen-Eleven Translocation 3
UseTagsflagExpressionMammalianMutationPromoterCMVAvailable sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
CMV-C 16 E7
Plasmid#85035PurposeC-Flag/HA tagged HPV 16 E7DepositorInsertHPV 16 E7 (E7 Human)
UseTagsFlag and HAExpressionMammalianMutationPromoterCMVAvailable sinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
T7-RelA(K310R)
Plasmid#23250DepositorInsertNFkB RelA (RELA Human)
UseTagsT7 gp10ExpressionMammalianMutationK310RPromoterAvailable sinceMarch 15, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAAV/L7-6-GFP-WPRE
Plasmid#126462PurposeAn AAV vector that expresses GFP under the control of the L7-6 promoter. The L7-6 promoter is the short size (0.8-kb) and has highly specificity to Purkinje cells.DepositorInsertL7-6, Purkinje cell specific promoter
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMay 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
SG3ΔENV CA M66I
Plasmid#149687PurposeNon-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS Reagent Program Cat #11051) and M66I mutation in capsid (CA M66I).DepositorInsertCapsid
UseLentiviral and RetroviralTagsExpressionMammalianMutationChanged Capsid residue Met 66 to Iso (CA M66I)PromoterAvailable sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
SG3ΔENV RT D185A
Plasmid#145782PurposeNon-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS Reagent Program Cat #11051) and D185A mutation in reverse transcriptase (RT D185A).DepositorInsertReverse Transcriptase
UseLentiviral and RetroviralTagsExpressionMammalianMutationChanged Reverse Transcriptase Aspartic Acid 185 t…PromoterAvailable sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-BiFC
Plasmid#87856PurposepETDUET-1 based vector for use in BiMolecular Fluorescence Complementation based assays. MCS at 5' of each BiFC fragment.DepositorInsertsEncodes for MCS & C-term half of mVenus
Encodes for MCS & N-term half of mVenus
UseTagsBiFC C-terminal fragmentExpressionBacterialMutationPromoterT7Available sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSynap-FLEX.SF-iGluSnFR.A184S
Plasmid#106180PurposeTight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorHas ServiceAAV5InsertSF-iGluSnFR.A184S
UseAAVTagsExpressionMutationGltI: A184SPromoterhSynapsinAvailable sinceJune 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
CN1402-rAAV-eHGT_058h-minBG-SYFP2-WPRE3-BGHpA
Plasmid#163494PurposeDirect-expressing SYFP2 AAV VirusInsertSYFP2
UseAAVTagsExpressionMutationPromoterminBetaGlobinAvailable sinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only