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Plasmid#175489PurposeProtein expression in bacterial cells. Tandem SH2 domains, M6-N269. Can be used for crystallography.DepositorInsertSYK (SYK Human)
UseTagsHis6-TEVExpressionBacterialMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV_GR-RE_MLPmin_BC1092-luc2
Plasmid#227112PurposeGlucocorticoid receptor response element for luciferase and barcode assays; barcode BC1092DepositorInsert12x clustered GR response element linked to MLP minimal promoter driving barcode 1092 and a luciferase reporter gene
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMN-334
Plasmid#195468PurposeLentiviral Integratable Inducible synZiFTR ZF10 + ReporterDepositorInsertinverted[8X ZF10 BS - ybTATA - mCherry] - pSFFV - ZF10 - NS3 - p65
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBad-EBFP2
Plasmid#14891PurposeBacterial expression of EBFP2 blue fluorescent proteinDepositorHas ServiceCloning Grade DNAInsertEBFP2
UseTagsHisExpressionBacterialMutationThe fluorescent protein was inserted between XhoI…PromoterAvailable sinceMay 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-myc3-CUL1
Plasmid#19896DepositorInsertcullin 1 (CUL1 Human)
UseTagsmyc3ExpressionMammalianMutationPromoterAvailable sinceJan. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
Flag-HA-USP53
Plasmid#22606DepositorInsertUSP53 (USP53 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationnot FL, missing first 65 aaPromoterCMV/TOAvailable sinceNov. 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-cGAS-HA
Plasmid#130910PurposeExpresses human cGAS-HA; Puromycin selection markerDepositorInsertcGAS
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCB591
Plasmid#141096PurposeE. coli-Lactobacilli shuttle vector for recombineering template cloningDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUCM-CLYBL-hNIL
Plasmid#105841PurposeDonor construct for introduction of hNIL factors to CLYBL safe harbor site and iPSC differentiation to motor neuronDepositorInsertsUseCRISPR and TALENTagsExpressionMutationPromoterCAG, EF-1alpha, and TRE3GAvailable sinceJuly 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-myc3-CUL2
Plasmid#19892DepositorInsertcullin 2 (CUL2 Human)
UseTagsmyc3ExpressionMammalianMutationPromoterAvailable sinceDec. 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W
Plasmid#67974PurposeCRISPR gRNA expression vector with an improved scaffold and puro/BFP markersDepositorInsertU6gRNA cassette, PGKpuro2ABFP cassette, WPRE
UseLentiviralTagsExpressionMutationDeleted BbsI site within WPREPromoterhuman U6 promoter, mouse PGK promoterAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
CMV-YE1-BE3-FNLS-CMV-mCherry
Plasmid#154005PurposeExpress YE1-BE3-FNLSDepositorInsertYE1-BE3-FNLS
UseCRISPRTagsFLAGExpressionMutationPromoterCMVAvailable sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLentiPGK Puro DEST JNKKTRClover
Plasmid#59151PurposeLentiviral vector to express JNK KTR mClover under PGK promoter (With Puromycin Resistance)DepositorInsertJNK Kinase Translocation Reporter (MAPK8 Human, Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterPGKAvailable sinceSept. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSCAR_sgRNA_hygro-tagBFP-lox5171
Plasmid#162070Purpose3rd generation lentivirus sgRNA delivery backbone with Cre-removable tagBFP reporter and hygromycin resistanceDepositorTypeEmpty backboneUseCRISPR, Cre/Lox, and LentiviralTagsExpressionMammalianMutationPromoterU6, EF1aAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMVTRE3G eGFP Blast (w818-1)
Plasmid#27568DepositorInsertEnhance Green Fluorescent Protein
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDEST_FLAG-HUWE1_C4341S
Plasmid#187156Purposemammalian cell expression FLAG-HUWE C4341SDepositorInsertHUWE1 (HUWE1 Human)
UseTags1xFLAGExpressionMammalianMutationC4341SPromoterAvailable sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
dCas13d-dsRBD-APEX2
Plasmid#154939PurposeTetON-APEX2-V5-BPNLS-dRfxCas13d-dsRBD-BPNLS-P2A-GFPDepositorInsertTetON-APEX2-V5-BPNLS-dRfxCas13d-dsRBD-BPNLS-P2A-GFP
UseTagsV5ExpressionMammalianMutationPromoterTRE3GAvailable sinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYHY3
Plasmid#216109PurposeaTC-inducible expression of secretory BT3630 SP-NanoLuc via P1TDP-A21 promoter-RBS pair in Bacteroides species.DepositorInsertNanoLuc
UseSynthetic Biology; E. coli-bacteroides shuttle ve…Tags3xFLAG tag and BT3630 signal peptideExpressionBacterialMutationPromoterP1TDP-A21Available sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-Rheb-N153T
Plasmid#19997DepositorInsertRas homolog enriched in brain 1 - N153T (RHEB Human)
UseTagsFLAGExpressionMammalianMutationChanged Asparagine 153 to ThreoninePromoterAvailable sinceJan. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pYD1
Plasmid#73447Purposeexpression vector designed for expression, secretion, and display of proteins on the extracellular surface of Saccharomyces cerevisiae cellsDepositorTypeEmpty backboneUseTags6xHis, AGA2, T7 peptide, V5 tag, and Xpress tagExpressionYeastMutationPromoterGAL1Available sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only