Addgene

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pBacPAK8-His-VHL-Flag

Plasmid

#29497

Depositor

Ronald Conaway, Joan Conaway

Article

Kamura et al Proc Natl Acad Sci U S A. 2000 Sep 12. 9

Insert

Von Hippel-Lindau (VHL Human)

Use

Tags

Flag and His

Expression

Insect

Mutation

Promoter

Available since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
UAS-Ceru-10unit

Plasmid

#158389

Purpose

10-unit intMEMOIR recording array for Drosophila melanogaster

Depositor

Michael Elowitz

Article

Chow et al Science. 2021 Apr 9;372(6538). pii: 372/

Insert

10unit recording array

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pZyxin-mMaple2

Plasmid

#101150

Purpose

Fluorescent protein for superresolution imaging and a focal adhesion protein for binding substrate

Depositor

Xiaowei Zhuang

Article

Wang et al Proc Natl Acad Sci U S A. 2014 Jun 10;11

Insert

human codon optimized mMaple2 fused to zyxin

Use

Tags

Expression

Mammalian

Mutation

Promoter

cmv

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pRSET FLIPE-1m

Plasmid

#13540

Depositor

Wolf Frommer

Article

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Insert

FLIP-E A207W

Use

Tags

ECFP and Venus

Expression

Bacterial

Mutation

A207W in binding protein

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt5B-V5

Plasmid

#35931

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt5B (WNT5B Human)

Use

Tags

V5

Expression

Mammalian

Mutation

None

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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Wnt9A STOP

Plasmid

#35881

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt9A (WNT9A Human)

Use

Entry vector

Tags

Expression

Mutation

Silent Mutation A (543) --> G

Promoter

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pSLQ1371_sgNanog

Plasmid

#63710

Purpose

Express sgNanog in mammalian cells of mice.

Depositor

Stanley Qi

Article

Yu et al Cell Stem Cell. 2015 Feb 5;16(2):142-7.

Insert

sgNanog, CMV promoter, puro, mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 27, 2015

Availability

Academic Institutions and Nonprofits only

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sox10:PDGFRA wild-type

Plasmid

#98696

Purpose

I-SceI meganuclease-mediated human PDGFRA wild-type transgenesis in zebrafish

Depositor

Thomas Look

Article

Ki et al Oncogene. 2017 Feb 23;36(8):1058-1068. d

Insert

PDGFRA (PDGFRA Human)

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

Available since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

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PEX-LV

Plasmid

#12120

Depositor

Inder Verma

Article

Pfeifer et al Proc Natl Acad Sci U S A. 2000 Oct 24. 9

Insert

Matrix Metalloproteinase 2 (Mmp2 Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

noncatalytic fragment of MMP2 comprised of the fi…

Promoter

Available since

April 27, 2007

Availability

Academic Institutions and Nonprofits only
pMD19T-psba1-Ppsba2-dCas9-SpR

Plasmid

#73220

Purpose

Contains dCas9 from S. pyogenes under constitutive promoter. Suicide vector inserts into psba1 site of Synechocystis. Carries spectinomycin resist. Recommend E. coli Copy cutter for propogation.

Depositor

Paul Hudson

Article

Yao et al ACS Synth Biol. 2015 Dec 28.

Insert

dCas9 from S. pyogenes

Use

Tags

c-myc

Expression

Bacterial

Mutation

Silent mutation at bp 1341 A->C to remove an E…

Promoter

PpsbA2

Available since

March 15, 2016

Availability

Academic Institutions and Nonprofits only

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pRSET FLIPglu-600uDelta11 Aphrodite

Plasmid

#13569

Depositor

Wolf Frommer

Article

Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

Insert

FLIPglu F16A Delta11 Aphrodite

Use

Tags

Aphrodite and ECFP

Expression

Bacterial

Mutation

Fluorophore-derived linker shortened. F16A mutati…

Promoter

Available since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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pGEX-HA-GlpG

Plasmid

#155135

Purpose

Expresses N-terminally GFP- and HA-tagged E. coli GlpG from pGEX 4P-1

Depositor

Sinisa Urban

Article

Cho et al Nat Struct Mol Biol. 2019 Oct;26(10):910

Insert

GlpG (glpG Escherichia coli)

Use

Tags

GST and HA

Expression

Bacterial

Mutation

Promoter

tac

Available since

Sept. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-Purg1-GFP

Plasmid

#39353

Depositor

Jurg Bahler

Article

Watt et al PLoS One. 2008 Jan 16;3(1):e1428.

Insert

urg1 promoter (urg1, urg1 Fission Yeast)

Use

Yeast genomic targeting

Tags

GFP and kanMX6 module - reverse orientation

Expression

Mutation

Promoter

Available since

Nov. 8, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA-Wnt7B-V5

Plasmid

#35934

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt7B (Wnt7b Human)

Use

Tags

V5

Expression

Mammalian

Mutation

None

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pCellFree_G06_ZIKV NS2A

Plasmid

#145353

Purpose

Cell-free expression vector for Zika virus NS2A protein with C-term mCherry

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS2A

Use

Tags

mCherry and His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pPB-DEST-R1R2-pNeo

Plasmid

#141057

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

Neomycin resistance gene

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAGM55361

Plasmid

#153227

Purpose

Control plasmid for knockout of AP3 gene

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Insert

Cas9, guide RNA for TRY and CPC and FAST marker

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-SGTAP no-pA

Plasmid

#82599

Purpose

3' entry vector containing streptavidin binding protein (SBP) followed by a TEV cleavage site and 2 protein G copies without pA for tandem affinity purification

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

SBP-TEV-2x protein G

Use

Tags

Expression

Mutation

Promoter

None

Available since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt9A-V5

Plasmid

#35937

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt9A (WNT9A Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Silent Mutation A (543) --> G

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pFLIPglu-3.2mDelta13

Plasmid

#12992

Depositor

Wolf Frommer

Article

Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

Insert

FLIPglu F16A S112A Delta13

Use

Plant nanosensor

Tags

ECFP and EYFP

Expression

Plant

Mutation

F16A, S112A in binding protein

Promoter

Available since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only