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NW11

Plasmid

#133443

Purpose

Covalently circularized nanodisc NW11 for studying membrane proteins and viral entry.

Depositor

Gerhard Wagner

Article

Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

Insert

NW11

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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Kras-Src FRET biosensor

Plasmid

#78302

Purpose

FRET biosensor. To monitor Src kinase activity in mammalian cells by FRET

Depositor

Yingxiao Wang

Article

Ouyang et al Proc Natl Acad Sci U S A. 2008 Sep 23;10

Insert

Src biosensor

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 22, 2016

Availability

Academic Institutions and Nonprofits only

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pET15b PKA Cat

Plasmid

#14921

Depositor

Susan Taylor

Article

Narayana et al Biochemistry. 1997 Apr 15. 36(15):4438-4

Insert

PKA catalytic subunit alpha (Prkaca Mouse)

Use

Tags

6xHis and Thrombin cleavage site

Expression

Bacterial

Mutation

Promoter

Available since

July 24, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt5A

Plasmid

#35911

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt5A (WNT5A Human)

Use

Tags

Expression

Mammalian

Mutation

None

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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NW9

Plasmid

#133442

Purpose

Covalently circularized nanodisc NW9 for studying membrane proteins and viral entry.

Depositor

Gerhard Wagner

Article

Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

Insert

NW9

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR_Puro_sgTP53

Plasmid

#187819

Purpose

pLentiCRISPR that is selectable with puromycin with an sgRNA targeting TP53

Depositor

William Kaelin

Article

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

sgTP53

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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NbV5-mCherry

Plasmid

#201474

Purpose

Indirect microscopy/imaging of V5-tagged protein

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

NbV5-mCherry

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCI HA NEDD4

Plasmid

#27002

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

NEDD4 (NEDD4 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

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eGFP STAT1 Y701F

Plasmid

#12302

Depositor

Alan Perantoni

Article

Timofeeva et al Oncogene. 2006 Jun 26. ():.

Insert

STAT1 Y701F (STAT1 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Y701F

Promoter

Available since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only
MSCVhygro-F-G9a

Plasmid

#41721

Purpose

Retroviral expression of human G9a in mammalian cells

Depositor

Kai Ge

Article

Wang et al EMBO J. 2012 Nov 23. doi: 10.1038/emboj.

Insert

G9a (EHMT2 Human)

Use

Retroviral

Tags

flag

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only

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LgBit-NbALFA

Plasmid

#201484

Purpose

NanoBit detection of ALFA-tagged protein

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

LgBit-NbALFA

Use

Tags

LgBit

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt4

Plasmid

#35910

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt4 (WNT4 Human)

Use

Tags

Expression

Mammalian

Mutation

Silent Mutation C (492) --> T

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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Wnt5A STOP

Plasmid

#35874

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt5A (WNT5A Human)

Use

Entry vector

Tags

Expression

Mutation

None

Promoter

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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Wnt4 STOP

Plasmid

#35873

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt4 (WNT4 Human)

Use

Entry vector

Tags

Expression

Mutation

Silent Mutation C (492) --> T

Promoter

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-GCaMP6s-CAAX

Plasmid

#52228

Purpose

Plasma membrane targeted GCaMP6s, calcium sensor

Depositor

Tobias Meyer

Article

Tsai et al Nat Cell Biol. 2014 Feb;16(2):133-44. do

Insert

GCaMP6s-CAAX

Use

Tags

6xHis and CAAX

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

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pEGFP_sfCherry2(11)_H2B

Plasmid

#82605

Purpose

Expresses sfCherry2(11) tagged H2B in mammalian cells

Depositor

Bo Huang

Article

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

sfCherry2(11)_H2B

Use

Tags

Expression

Mammalian

Mutation

changed Gly12 to Ala in sfCherry11+

Promoter

Available since

June 29, 2017

Availability

Academic Institutions and Nonprofits only

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NbAlfa-LgBit

Plasmid

#201483

Purpose

NanoBit detection of ALFA-tagged protein

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

NbALFA-LgBit

Use

Tags

LgBit

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt3A

Plasmid

#35908

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt3A (WNT3A Human)

Use

Tags

Expression

Mammalian

Mutation

None

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt1

Plasmid

#35905

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt1 (WNT1 Human)

Use

Tags

Expression

Mammalian

Mutation

None

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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human STIM1 YFP

Plasmid

#19754

Depositor

Anjana Rao

Article

Prakriya et al Nature. 2006 Sep 14. 443(7108):230-3.

Insert

STIM1 (STIM1 Human)

Use

Retroviral

Tags

YFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only

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