NbAlfa-LgBit
(Plasmid
#201483)
-
PurposeNanoBit detection of ALFA-tagged protein
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 201483 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepcDNA6/ V5-HisA
-
Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5502
-
Vector typeMammalian Expression
-
Selectable markersBlasticidin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameNbALFA-LgBit
-
SpeciesSynthetic
-
Insert Size (bp)918
- Promoter CMV
-
Tag
/ Fusion Protein
- LgBit (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer Bgh-R (Common Sequencing Primers)
Resource Information
-
A portion of this plasmid was derived from a plasmid made bySynthetic
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
NbAlfa-LgBit was a gift from Patrick Giguère (Addgene plasmid # 201483 ; http://n2t.net/addgene:201483 ; RRID:Addgene_201483) -
For your References section:
Development of a V5-tag-directed nanobody and its implementation as an intracellular biosensor of GPCR signalling. Zeghal M, Matte K, Venes A, Patel S, Laroche G, Sarvan S, Joshi M, Rain JC, Couture JF, Giguere PM. J Biol Chem. 2023 Jul 28:105107. doi: 10.1016/j.jbc.2023.105107. 10.1016/j.jbc.2023.105107 PubMed 37517699