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Plasmid#8830DepositorInsertTEV protease, S219P mutant
UseTagsHis, MBP (cleaved), and polyarginineExpressionBacterialMutationS219P mutationPromoterAvailable sinceJuly 18, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGEM4Z-EGFP
Plasmid#183475PurposeThis plasmid can be used to prepare EGFP mRNA by In Vitro TranscriptionDepositorInsertEGFP
UseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEXP5-NT/pT7 LuxI
Plasmid#193626PurposeConstitutive T7 RNAP-mediated expression of LuxI 3OC6-HSL synthase for Lux quorum sensing system. LuxI sequence is codon optimized for E. coli.DepositorInsertLuxI
UseTags6xHisExpressionBacterialMutationPromoterT7 RNAP promoterAvailable sinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
M-HBsAg
Plasmid#103012PurposeExpresses HBV Medium envelope glycoprotein in mammalian cellsDepositorInsertHBV Medium envelope protein
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCX-V5-H7N9-NS1
Plasmid#99022PurposeMammalian expression of NS1DepositorInsertNS1
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
YIp128-P30-His-pol30(K164R)
Plasmid#99544PurposeYeast integrative vector for expression of His-tagged POL30 (PCNA) mutant K164R; Leu2 markerDepositorInsertPOL30 (POL30 Budding Yeast)
UseTags6xHisExpressionYeastMutationK164RPromoterPOL30Available sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX-Cdc42
Plasmid#12201DepositorInsertCdc42 (CDC42 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceAug. 11, 2006AvailabilityAcademic Institutions and Nonprofits only -
543-p3E-2A-mcherrypA
Plasmid#26031DepositorInsertmCherry
UseEntry vectorTagsExpressionMutationPromoterAvailable sinceAug. 9, 2010AvailabilityAcademic Institutions and Nonprofits only -
p3E-P2A-mKate2 no-pA
Plasmid#80828PurposeP2A followed by mKate2 without pADepositorInsertp3E-P2A-mKate2 no pA
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLH-sgRNA-Sirius-8XPP7
Plasmid#121940PurposeCRISPR-Sirius plasmidDepositorInsertsgRNA-Sirius-8XPP7
UseCRISPR and LentiviralTagsnoExpressionMammalianMutationPromoterhuman U6 promoterAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
V249 pCEP-4HA B56delta
Plasmid#14536DepositorInsertPPP2R5D B56 delta regulatory subunit of PP2A (PPP2R5D Human)
UseTags4 HAExpressionMammalianMutationR210HPromoterAvailable sinceMarch 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-ro2GFP
Plasmid#82370PurposeExpression of redox sensitive GFP in mammalian cellsDepositorInsertro2GFP
UseTagsExpressionMammalianMutationC48S/F64L/S147C/Q204CPromoterCMVAvailable sinceNov. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_PTBP1-Halo
Plasmid#175302PurposeProduction of HaloTag fusion proteinDepositorInsertPTBP1 (PTBP1 Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUCHR-inLuc
Plasmid#58955PurposeHIV-1 transfer vector, encodes firefly luciferase gene interrupted by a gamma-globin intron. The reporter gene is silent in the transfected cells and expressed in the infected cells.DepositorInsertinLuc
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceSept. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pDisplay-Rncp-iGluSnFR1
Plasmid#107336PurposeRed fluorescent glutamate sensor with ncp topology. Membrane-displayedDepositorInsertRncp-iGluSnFR1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCIP
Plasmid#79009PurposeFor expression in mouse ESCs or other eukaryotic cells. Contains pCAGGS-IRES-Puro-bpA, with a NheI-MfeI cloning site in between pCAGGS and IRES.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterpCAGGsAvailable sinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDAS12222_U6-pegRNA-BFP
Plasmid#177181PurposepegRNA cloning plasmid with BFP markerDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR - AAVS1 sgRNA
Plasmid#70661PurposeExpresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an AAVS1-targeting sgRNA element from U6 promoter. Lentiviral backboneDepositorInsertsgRNA against AAVS1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
FUW-RLuc-T2A-PuroR
Plasmid#102320Purposeexpression of r-luciferaseDepositorInsertRenilla luciferase
UseLentiviralTagsExpressionMutationPromoterUbcAvailable sinceJuly 13, 2018AvailabilityAcademic Institutions and Nonprofits only