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Plasmid#68252PurposeLevel 1 Golden Gate Cassette: Plant kanamycin resistance cassetteDepositorInsertPromoter:2x35s+5'UTR:Omega+CDS:nptII+3'UTR/terminator:Nos
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceSept. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHR-CMV-TetO2_HA-BirA-ER
Plasmid#113897PurposeMammalian (inducible) expression of HA-tagged ER-resident E. coli biotin ligaseDepositorInsertE. coli biotin ligase
UseLentiviralTagsHAExpressionMutationPromoterCMV-MIE-TetO2Available sinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
MiniCoopR U6:gRNA spred1, mitfa:Cas9
Plasmid#118843PurposeTargets spred1 and expresses zebrafish mitfa specifically in zebrafish melanocytesDepositorInsertspred1 gRNA (spred1 Zebrafish)
UseCRISPR; Tol2TagsExpressionMutationPromoterAvailable sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
HOXB4 (human) pSG5
Plasmid#8527DepositorInsertHOXB4 (HOXB4 Human)
UseIn vitro transcriptioin/translationTagsExpressionMammalianMutationPromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-dDIO-vCre
Plasmid#118029PurposeAAV vector for sparse and bright labeling in a cell-type specific mannerDepositorInsertTRE-dDIO-vCre
UseAAVTagsExpressionMutationSee depositor comments belowPromoterTREAvailable sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
px330-GAPDH
Plasmid#136940PurposeNHEJ assay. sgRNA/Cas9 plasmid. Target DSB at human GAPDH; induce CD4+ deletion rearrangement by pairing w/ px330-CD4DepositorInsertsgRNA targeting GAPDH (GAPDH Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEJS1004-pCSDest2-AcrIIA5-NLS-FLAG
Plasmid#136513PurposeExpresses type II-A anti-CRISPR protein AcrIIA5 in mammalian cellsDepositorInsertcodon-optimized AcrIIA5
UseTagsFLAG/NLSExpressionMammalianMutationPromoterCMVAvailable sinceApril 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENN.AAV.CB7.CI.CNDP2-flag.WPRE.rBG
Plasmid#132685PurposeAAV plasmid encoding mouse CNDP2 with C-terminal flag tagDepositorInsertCarnosine dipeptidase 2 (Cndp2 Mouse)
UseAAVTagsFlagExpressionMutationPromoterChicken b-actinAvailable sinceNov. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
Neuroplastin-CD4d3+4-bio
Plasmid#73111PurposeEXPRESs plasmid for human erythrocyte surface proteins encoding Neuroplastin with rat CD4d3+4-bioDepositorInsertNeuroplastin (NPTN Human)
UseTagsbiotinylation peptide and ratCD4d3+4ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-dDIO-lck-smMyc-4x6T
Plasmid#196421PurposeDre-dependent AAV expression of membrane-targeted Myc spaghetti monster reporter preferentially in astrocytes; astrocyte selectivity generated with 4x6T miRNA targeting cassetteDepositorInsertLck-smMyc
UseAAV; Dre/rox; astrocyte-selectiveTagsLckExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet-H_H2A_C1
Plasmid#85052PurposeIn vivo visualization of the nucleus (can be used for colocalization studies)DepositorInsertH2A (H2AC16 Human)
UseTagsmScarlet-HExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
EF1a_KLF7_P2A_Hygro_Barcode
Plasmid#120493PurposeBarcoded lentiviral vector to express KLF7 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertKLF7 (KLF7 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
PH-PLCD1_mScarlet-H_IRES_SYFP2_PH_N1
Plasmid#85070PurposeYellow/red FRET biosensor for monotoring plasmamembrane PtdIns(4,5)P2 levels (PIP2)DepositorInsertPH-PLCD1 (PLCD1 Human)
UseTagsIRES-sYFP2-PH and mScarlet-HExpressionMammalianMutationPLCD aa 1–170PromoterCMVAvailable sinceMarch 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
TET-pLKO.1 PURO shID2 #2
Plasmid#83087PurposeLentiviral shRNA vector for inducible knockdown of human ID2DepositorInsertshID2 (ID2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
YFP-FKBPx5-His
Plasmid#103785Purposeexpresses His-tagged iPOLYMER component in E. coli for protein purificationDepositorInsertYFP-FKBPx5-His
UseTagsEYFP, His-tagExpressionBacterialMutationPromoterT7Available sinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
kiCAP-AAV-PAL1C
Plasmid#196685PurposeRep/Cap plasmid for the production of PAL1C, an AAV capsid with CNS tropism in macaques.DepositorInsertAAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
UseAAVTagsExpressionMutationPTQGTLR insert between amino acids 588 and 589 of…Promoterp41Available sinceApril 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
EF1a_LMX1A_P2A_Hygro_Barcode
Plasmid#120457PurposeBarcoded lentiviral vector to express LMX1A in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertLMX1A (LMX1A Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLX302 ID2-V5 puro
Plasmid#83096PurposeLentiviral vector for constitutive expression of human ID2 with C-terminal V5 tagDepositorInsertID2 (ID2 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-TWIN-STREP-FLAG-NPRL3
Plasmid#164984PurposeNPRL3 expressing vector for mammalian cellsDepositorInsertNPRL3 (NPRL3 Human)
UseTags2xSTREP FLAGExpressionMammalianMutationPromoterCAGAvailable sinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti6/V5-DEST-RINT1
Plasmid#40124DepositorInsertRINT1 (RINT1 Human)
UseLentiviralTagsV5 epitopeExpressionMammalianMutationPromoterCMVAvailable sinceJuly 16, 2013AvailabilityAcademic Institutions and Nonprofits only