-
Plasmid#18982DepositorInsertHistone H2B monomeric red fluorescent protein
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 24, 2008AvailabilityAcademic Institutions and Nonprofits only -
-
pCL-CTIG
Plasmid#14901Purpose3rd generation lentiviral vector expressing tTA-VP16 and EGFP.DepositorInsertCMV-tTA-GFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2007AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-25:MAP1LC3B-mEGFP
Plasmid#101783PurposeHomology arms and mEGFP-linker sequence for N-terminus tagging of human MAP1LC3BDepositorInsertMAP1LC3B Homology Arms with mEGFP-linker (MAP1LC3B Human)
UseCRISPR; Donor templateTagsmEGFP-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceOct. 31, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
330-Plakoglobin-Myc
Plasmid#32228DepositorInsertPlakoglobin (JUP Human)
UseTagsMycExpressionMammalianMutationPromoterhuman beta-actinAvailable sinceOct. 26, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-4mtD3cpv
Plasmid#36324DepositorInsert4mtD3cpv second generation cameleon (calcium sensor) targeted to mitochondria
UseTagsFirst 29 aa of human COX subunit 8aExpressionMutationPromoterCMVAvailable sinceAug. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pNCST-avGFP
Plasmid#129508PurposeExpresses avGFP (Lambert 2019 clone) constitutively in E. coli (most strains)DepositorInsertavGFP
UseTagsExpressionBacterialMutationPromotersynthetic constitutive (stationary phase) promote…Available sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
RabV CVS-N2c(deltaG)-tdTomato
Plasmid#73462PurposeExpresses tdTomato for monosynaptic circuit mappingDepositorInserttdTomato
UseNeurotropic virusTagsExpressionMutationPromoterAvailable sinceMarch 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
-
NSK026 CMV-TO-p53R248W (FLP-IN)
Plasmid#191168PurposeExpression of human p53 mutant R248W in mammalian cellsDepositorInsertp53(R248W) (TP53 Human)
UseTagsExpressionMammalianMutationR248WPromoterCMV-TOAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
Intron-Tagging-pX330-Cas9-Blast
Plasmid#159741PurposeExpresses Cas9-2A-Blast and a sgRNA excising EGFP flanked by a splice acceptor and a splice donor from the Intron-Tagging-EGFP-Donor plasmid.DepositorInsertdonor plasmid targeting sgRNA
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCIP-caTfeb
Plasmid#79013PurposeFor mammalian expression of a shortened, constitutively active version of Tfeb.DepositorInsertTfeb (TFEB Human)
UseTagsExpressionMammalianMutationRemoved first ~400 bp from 5' end (N-terminu…PromoterpCAGGsAvailable sinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
IBMc396
Plasmid#161954PurposeDemonstrates chemically induced dimmerization of acVHH. Expression plasmid for split, evolved T7 RNA polymerase N-lobe fused to acVHHDepositorInsertpSEVA321-PBAD(SapI-)-B30-His-T7RNAP(29-1)-15aa-acVHH-T
UseTagsExpressionBacterialMutationPromoterPBADAvailable sinceMarch 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1_SPTLC1_WT
Plasmid#181920Purpose3rd generation, Inducible lentiviral plasmid for expression of human wildtype SPTLC1DepositorInsertHuman serine palmitoyltransferase, long chain base subunit 1 (SPTLC1), (SPTLC1 Human)
UseLentiviral; Mammalian expression, , doxycycline i…TagsExpressionMammalianMutationPromotertight TRE promoterAvailable sinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLG004 (RT-Abi-P2)
Plasmid#157882PurposeExpresses the RT-Abi-P2 defense systemDepositorInsertRT-Abi-P2
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
IDG_TMC3_OE_1
Plasmid#161629PurposeOverexpresses TMC3DepositorInsertTMC3 (TMC3 Human)
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-AcGFP1-ORP9-PH
Plasmid#214266PurposeMammalian expression of green fluorescent PI4P reporter AcGFP1-ORP9-PHDepositorArticleInsertAcGFP1-ORP9(1-111) (Osbpl9 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-PE2-His (IK1822)
Plasmid#170103PurposeT7 promoter bacterial expression plasmid for PE2 (nSpCas9(H840A)-MMLV-RT) with C-terminal 6xHis-tag and bipartite NLSDepositorInsertbpNLS-nSpCas9(H840A)-MMLVRT-bpNLS-6xHis
UseCRISPRTags6xHis tagExpressionBacterialMutationBacteria codon-optimized nSpCas9 (H840A) & M-…PromoterT7Available sinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETduet+p300HAT
Plasmid#157793PurposeExpression of p300 HAT domainDepositorInsertp300HAT (EP300 Human)
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-PM
Plasmid#197386PurposeTo constitutively express Pdx1-Mafa and in cells (Blasticidin resistant).DepositorInsertPDX1-T2A-MAFA
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
-
-
HA-TAZ-S89A
Plasmid#32840DepositorInsertTAZ (WWTR1 Human)
UseTagsHAExpressionMammalianMutationS89APromoterCMVAvailable sinceNov. 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCAM-kan-R4R3
Plasmid#71275PurposeBinary destination vector for plant transformation. Kanamycin resistance.DepositorTypeEmpty backboneUseMultisite gatewayTagsExpressionMutationPromoterAvailable sinceDec. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDonor_sU6
Plasmid#69351PurposesgRNA scaffold and synthetic U6 promoterDepositorInsertsU6 promoter and sgRNA scaffold flanked by BbsI sites
UseCRISPRTagsExpressionMutationPromotersynthetic U6 promoterAvailable sinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pU6a:sgRNA#1
Plasmid#64245Purposeexpresses sgRNA under U6a promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
FUW-hTie1-FLAG
Plasmid#119233PurposePlasmid encodes FLAG-tagged full-length human Tie1 gene cloned in lentiviral vector.DepositorInsertTIE1 (TIE1 Human)
UseLentiviralTagsFLAGExpressionMutationPromoterP hUbCAvailable sinceDec. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
Lenti-TargetBC-5xTAPE-1-pegRNA-InsertBC
Plasmid#183790PurposeA single construct out of the pool of plasmid for lineage recording experiment using DNA Ticker Tape.DepositorInsertsP2A-eGFP-TargetBC-5xTAPE-1
pegRNA-InsertBC
UseSynthetic BiologyTagsExpressionMutationG>A in the gRNA scaffold- please see depositor…PromoterAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDragon-p21
Plasmid#155016PurposeFor Flp-mediated cassette exchange. Expresses tdTomato in the presence of (r)tTA, switching to the cell cycle suppressor p21 in the presence of Cre and (r)tTA activityDepositorInsertCdkn1a (Cdkn1a Mouse)
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pK031.TRE-Cre (Supernova)
Plasmid#69136PurposeFor the Supernova, a series of vector systems for single-cell labeling and gene function sudies in vivoDepositorInsertNLS-Cre
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only