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Plasmid#58310PurposeShuttle vector: pUC ori, pTHT15 ori for use in E. coli/B. subtilis. Cannot modify orientation of cat gene; promoter too weak in E. coli, probably transcribed via repB promoter in bicistronic manner.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 29, 2015AvailabilityAcademic Institutions and Nonprofits only
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pBABE puro mouse IRS-2 myc
Plasmid#11373DepositorInsertIRS-2 (Irs2 Mouse)
UseRetroviralTagsmycExpressionMammalianMutationPromoterAvailable sinceMay 25, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-vhhGFP4-hIgG1-Fc
Plasmid#105576PurposeT7 promotor drives in vitro mRNA transcription of GFP nanobody-Fc antibody domain fusionDepositorInsertHuman IgG1 Fc-domain fused with GFP nanobody (IGHG1 Human)
UseIn vitro transcriptionTagsExpressionMutationPromoterAvailable sinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-VEGFR1-V5/HIS
Plasmid#205616PurposeExpression of human VEGFR1 receptor tyrosine kinase in mammalian cellsDepositorInsertVEGFR1 receptor tyrosine kinase (FLT1 Human)
UseTagsV5-HisExpressionMammalianMutationPromoterCMVAvailable sinceSept. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP-P4M-SidMx2
Plasmid#51472PurposeBiosensor for PtdIns4PDepositorInsertsP4M-SidM(546-647)
P4M-SidM(546-647)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRE57-Ter
Plasmid#170635PurposeDNA donor for transpositionDepositorInsertMini-Tn6677, Ter cassette
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MH6-Bsu
Plasmid#163911PurposeExpresses Bsu large fragment for affinity purificationDepositorInsertBsu polymerase
UseTagsHisExpressionBacterialMutationPromoterT7 promoterAvailable sinceMarch 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLPC-Flag-PML-IV
Plasmid#62804Purposeexpresses the isoform IV of PML (promyelocytic leukemia protein)DepositorInsertPML-IV (PML Human)
UseRetroviralTags3X-FLAGExpressionMammalianMutationPromoterAvailable sinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-hcGAS(157-522)
Plasmid#108676PurposeFor expression in E.coli and affinity purification of N-terminally GST-tagged human cGAS amino acid 157-522DepositorInsertcGAS amino acid 157-522 (CGAS Human)
UseTagsGSTExpressionBacterialMutationcontains amino acid 157-522PromotertacAvailable sinceApril 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pME-mCherry-24xMBS
Plasmid#86242PurposeMiddle entry vector for generating MBS tagged RNAs with a fluorescent protein reporterDepositorTypeEmpty backboneUseUnspecifiedTagsmCherry-24xMBSExpressionMutationPromoterAvailable sinceFeb. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCX-OKS-2A
Plasmid#19771PurposeNon-integrating (episomal) expression of mouse Oct3/4, Klf4 and Sox2DepositorUseTagsExpressionMammalianMutationThree genes are connected with the foot-and-mouth…PromoterAvailable sinceJan. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
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EBFP2-pBAD
Plasmid#54542PurposeLocalization: Protein Expression Vector , Excitation: 383, Emission: 448DepositorTypeEmpty backboneUseTags6xHis and EBFP2ExpressionBacterialMutationPromoterAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCWU6-xylR-E-luciferase
Plasmid#190721Purposefine tune expression of luciferase under the control of xylose- and theophylline- inducible systemDepositorInsertxylR-E-luciferase
UseLuciferaseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_BFP
Plasmid#25891DepositorInsertBFP
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLTR.gp140/EGFP.Revdelta38/DsRed
Plasmid#115775PurposeHIV splicing reporter allows the assessment of the effect of LRAs on HIV-1 transcription and splicing.DepositorInsertEnvelope fused to EGFP (gp140-EGFP) and non-functional Rev protein fused to DsRed fluorescent protein (Rev-delta38-DsRed).
UseTagsEGFP; dsReDExpressionMammalianMutationgp140 uncleaved (1190-91aa, KR > TG; truncated…PromoterHIV-1 (LTR)Available sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTRK669
Plasmid#71313PurposeHelper plasmid for pORI plasmids. Chloramphenicol resistant, temperature sensitive replication in E. coli and gram positives. Encodes RepA.DepositorTypeEmpty backboneUseE. coli-gram positive bacteria shuttle vectorTagsExpressionBacterialMutationPromoterAvailable sinceJan. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
mCer-TG2-YFP
Plasmid#85471PurposeTG2 biosensor that allows for quantitative assessment of TG2 conformational changes in live cells using FRETDepositorInserthTG2 (TGM2 Human)
UseTagsEYFP and mCeruleanExpressionMammalianMutationPromoterCMVAvailable sinceDec. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pST_140_LVL2 cam
Plasmid#179334PurposeNT-CRISPR plasmid for a single gRNA with spG Cas9 (near PAM-less Cas9 variant).DepositorInsertPtac tfoX, Ptet spG cas9, PJ23106 acrIIA4, Ptet gRNA with sfGFP dropout
UseCRISPRTagsExpressionMutationCas9 -> SpG Cas9 (D1135L, S1136W, G1218K, E121…PromoterAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
MP2
Plasmid#69628PurposeUntargetted Bacterial MutagenesisDepositorInsertdnaQ926
UseTagsExpressionBacterialMutationPromoterE. coli PBADAvailable sinceSept. 21, 2015AvailabilityAcademic Institutions and Nonprofits only