Addgene

We narrowed to 28,633 results for: uros

Showing: 6381 - 6400 of 28633 results

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pTRE/SP M1WT

Plasmid

#89327

Purpose

Inducible expression of WT M1 spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al J Neurosci. 2014 Jan 29;34(5):1856-67. d

Insert

spastin (SPAST Human)

Use

Tags

Expression

Mammalian

Mutation

1-194 bp of 5'UTR deleted

Promoter

Tet-responsive P tight

Available since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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pTRE/SP M87 WT

Plasmid

#89328

Purpose

Inducible expression of WT M87 spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al J Neurosci. 2014 Jan 29;34(5):1856-67. d

Insert

spastin (SPAST Human)

Use

Tags

Expression

Mammalian

Mutation

1-460 bp deleted

Promoter

Tet-responsive P tight

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pX28

Plasmid

#46850

Purpose

TOP RFP op. PGK-puro indicator plasmid to assay TOP IMCE efficiency

Depositor

Monica Hollstein

Article

Wei et al Cell Cycle. 2011 Apr 15;10(8):1261-70. E

Insert

attB, RFP and PGK-puro

Use

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Aug. 21, 2013

Availability

Academic Institutions and Nonprofits only

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pGBT9-chNfasc (+RSLE)

Plasmid

#89418

Purpose

Two-hybrid system

Depositor

Fritz Rathjen

Article

Koroll et al J Biol Chem. 2001 Apr 6;276(14):10646-54

Insert

NFASC (NFASC Chicken)

Use

Tags

GAL4 DBD

Expression

Yeast

Mutation

Promoter

ADH1

Available since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

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Fast-GCaMP-RS14

Plasmid

#52651

Purpose

genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

Depositor

Samuel Wang

Article

Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

Insert

Fast-GCaMP-RS14

Use

Tags

Expression

Mammalian

Mutation

deleted G47 from GCaMP3

Promoter

T7

Available since

May 6, 2014

Availability

Academic Institutions and Nonprofits only
Fast-GCaMP-RS06

Plasmid

#52647

Purpose

genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

Depositor

Samuel Wang

Article

Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

Insert

Fast-GCaMP-RS06

Use

Tags

Expression

Mammalian

Mutation

M374Q mutated from GCaMP3

Promoter

T7

Available since

April 23, 2014

Availability

Academic Institutions and Nonprofits only
YFP-HA-DAT W63A

Plasmid

#90246

Purpose

Expresses YFP-HA-DAT W63A mutant in mammalian cells

Depositor

Alexander Sorkin

Article

Sorkina et al J Neurosci. 2009 Feb 4;29(5):1361-74. do

Insert

DAT (dopamine transporter) (SLC6A3 Human)

Use

Tags

Expression

Mammalian

Mutation

inserted HA tag (see YFP-HA-DAT), mutated W63 to A

Promoter

CMV

Available since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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YFP-HA-DAT R60A

Plasmid

#90245

Purpose

Expresses YFP-HA-DAT R60A mutant in mammalian cells

Depositor

Alexander Sorkin

Article

Sorkina et al J Neurosci. 2009 Feb 4;29(5):1361-74. do

Insert

DAT (dopamine transporter) (SLC6A3 Human)

Use

Tags

Expression

Mammalian

Mutation

inserted HA tag (see YFP-HA-DAT), mutated R60 to A

Promoter

CMV

Available since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pVenus-PICK1-shRNA

Plasmid

#72575

Purpose

Expresses shRNA against PICK1 as well as the marker Venus fluorescent protein

Depositor

Richard Huganir, Victor Anggono

Article

Anggono et al J Neurosci. 2011 Feb 9;31(6):2188-96. do

Insert

PICK1 (Pick1, Pick1 Rat, Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

H1 promoter

Available since

April 28, 2017

Availability

Academic Institutions and Nonprofits only
6his R.3-AR GUSpET28a+/pLysS

Plasmid

#61157

Purpose

N-terminal His tagged round 3 aldehyde-resistant GUS expression vector

Depositor

Ichiro Matsumura, Andrew Ellington

Article

Matsumura et al Nat Biotechnol. 1999 Jul;17(7):696-701.

Insert

GUSAR

Use

Tags

6x His

Expression

Bacterial

Mutation

N66D, D151N, A219V, I396T, T480A, Q498R, D508E an…

Promoter

T7

Available since

March 17, 2015

Availability

Academic Institutions and Nonprofits only

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pWJPrP1

Plasmid

#22109

Depositor

Susan Lindquist

Article

Jackson et al Neuron. 2009 Aug 27. 63(4):438-50.

Insert

mouse PrP ORF insert (Prnp Mouse)

Use

Construction of targeting vector

Tags

Expression

Mutation

mutations at aa 108 and 111 to make 3F4 epitope (…

Promoter

Available since

Oct. 16, 2009

Availability

Academic Institutions and Nonprofits only
pCMV Lex VP16 (F442A) HA (P#1710)

Plasmid

#14595

Depositor

Matija Peterlin

Article

Kurosu et al Curr Biol. 2004 Jun 22. 14(12):1112-6.

Insert

LexA

Use

Tags

HA and VP16 (F442A)

Expression

Mammalian

Mutation

The VP16 sequence contains an F442A mutation. The…

Promoter

Available since

June 30, 2008

Availability

Academic Institutions and Nonprofits only
Fast-GCaMP-RS12

Plasmid

#52650

Purpose

genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

Depositor

Samuel Wang

Article

Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

Insert

Fast-GCaMP-RS12

Use

Tags

Expression

Mammalian

Mutation

deleted R40/R41/K42 from GCaMP3

Promoter

T7

Available since

May 6, 2014

Availability

Academic Institutions and Nonprofits only
pCRRNA-G0-R20

Plasmid

#101818

Purpose

Repress mCherry and GFP independently, with different strengths

Depositor

David Bikard, Sven van Teeffelen

Article

Vigouroux et al Mol Syst Biol. 2018 Mar 8;14(3):e7899.

Insert

crRNAs targeting GFP and mCherry

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Spy 1049

Available since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pCRRNA-G20-R0

Plasmid

#101807

Purpose

Repress mCherry and GFP independently, with different strengths

Depositor

David Bikard, Sven van Teeffelen

Article

Vigouroux et al Mol Syst Biol. 2018 Mar 8;14(3):e7899.

Insert

crRNAs targeting GFP and mCherry

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Spy 1049

Available since

Oct. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pISH-Gucy1b2-B

Plasmid

#105457

Purpose

to generate a riboprobe for in situ hybridazation

Depositor

Peter Mombaerts

Article

Bleymehl et al Neuron. 2016 Dec 21;92(6):1196-1203. doi

Insert

Gucy1b2 (Gucy1b2 Mouse)

Use

To ganerate riboprobe

Tags

Expression

Mutation

Promoter

Available since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pISH-Gucy1b2-A

Plasmid

#105456

Purpose

to generate a riboprobe for in situ hybridazation

Depositor

Peter Mombaerts

Article

Bleymehl et al Neuron. 2016 Dec 21;92(6):1196-1203. doi

Insert

Gucy1b2 (Gucy1b2 Mouse)

Use

To ganerate riboprobe

Tags

Expression

Mutation

Promoter

Available since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pWJPrP5

Plasmid

#22106

Depositor

Susan Lindquist

Article

Jackson et al Neuron. 2009 Aug 27. 63(4):438-50.

Insert

mouse PrP ORF insert (Prnp Mouse)

Use

Construction of targeting vector

Tags

Expression

Mutation

mutated codon 177 (D to N)

Promoter

Available since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only
Fast-GCaMP-EF09

Plasmid

#52640

Purpose

genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

Depositor

Samuel Wang

Article

Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

Insert

Fast-GCaMP-EF09

Use

Tags

Expression

Mammalian

Mutation

D366N mutation from GCaMP3 sequence

Promoter

T7

Available since

April 23, 2014

Availability

Academic Institutions and Nonprofits only
Fast-GCaMP-EF15

Plasmid

#52641

Purpose

genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

Depositor

Samuel Wang

Article

Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

Insert

Fast-GCaMP-EF15

Use

Tags

Expression

Mammalian

Mutation

D431N/D433N/D435N mutated from GCaMP3

Promoter

T7

Available since

April 23, 2014

Availability

Academic Institutions and Nonprofits only