-
Plasmid#208642PurposeEmpty vector for long single strand DNA (up to 1.5k) preparation. This plasmid is a pUC19 with 6 point mutations that remove BspQI, BtsI, BsrDI sites. Mutations adopted from Patent WO/2018/015995.DepositorTypeEmpty backboneUseLong single strand dna productionTagsExpressionMutationPromoterAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only
-
pET28-5'TwStrep
Plasmid#208620PurposeEmpty vector for prokaryotic expression, insert a Twin Strep tag between NdeI and BamHI site of pET28a (+), both site retained, T7 tag replaced, BamHI site in-frame with Twin Strep tag.DepositorTypeEmpty backboneUseTagsTwin StrepExpressionBacterialMutationPromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
Cas9_P2A_T4pol
Plasmid#190146PurposeExpresses SpCas9 and T4polDepositorInsertSpCas9_P2A_T4pol
UseCRISPRTags3xFLAGExpressionMammalianMutationCodon Optimized for mammalian cellsPromoterCBhAvailable sinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLZRS-TrkC-mCherry
Plasmid#182949PurposeTo express the rat TrkC fused in the C-terminus with mCherryDepositorInsertTRk-C-mCherry (Ntrk3 Rat, Synthetic)
UseRetroviral and Synthetic BiologyTagsmCherryExpressionMammalianMutationPromoterMMLVAvailable sinceMay 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmr101A-cPr-mNeonGreen (ErmR)
Plasmid#177208PurposePlasmid with p15A origin for constitutive mNeonGreen expression in E. coliDepositorInsertmNeonGreen
UseTagsExpressionBacterialMutationYeast codon optimizedPromoterSynthetic constitutive promoterAvailable sinceMarch 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
AtALMT1-upGFP 3'
Plasmid#117889Purposeprotein expressionDepositorInsertAtALMT1-upGFP 3' (ALMT1 Mustard Weed)
UseTagseGFPExpressionYeastMutationPromoterAOXAvailable sinceDec. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
AtALMT1-upGFP 5'
Plasmid#117887Purposeprotein expressionDepositorInsertAtALMT1-upGFP 5' (ALMT1 Mustard Weed)
UseTagseGFPExpressionYeastMutationPromoterAOXAvailable sinceDec. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJK640
Plasmid#72467PurposeEncodes Escherichia coli evgADepositorInsertresponse regulator in two-component regulatory system with EvgS (evgA Escherichia coli str. K-12 substr. MG1655)
UseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK641
Plasmid#72468PurposeEncodes Escherichia coli evgA (Plac noncoding)DepositorInsertresponse regulator in two-component regulatory system with EvgS (evgA Escherichia coli str. K-12 substr. MG1655)
UseTagsExpressionBacterialMutationPromoterlacAvailable sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK646
Plasmid#72469PurposeEncodes Escherichia coli evgA (Plac coding)DepositorInsertresponse regulator in two-component regulatory system with EvgS (evgA Escherichia coli str. K-12 substr. MG1655)
UseTagsExpressionBacterialMutationPromoternoneAvailable sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBaKdoO
Plasmid#36285DepositorInsertbamb_0774
UseTagsExpressionMutationPromoterAvailable sinceJuly 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
pYpKdoO.1
Plasmid#36286DepositorInserty1812
UseTagsExpressionMutationPromoterAvailable sinceMay 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCM1.95
Plasmid#21381DepositorInsertlip-1 M1M2 3'utr in pDONRP2RP3
UseGatewayTagsExpressionMutationPromoterAvailable sinceJune 19, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCMV4-3HA
Plasmid#24165DepositorTypeEmpty backboneUseTags3xHAExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Flag
Plasmid#208051PurposeEmpty vector for mammalian expression, insert a Flag tag (with ATG) at XhoI site of pcDNA3.1 (-), xhoI site at 5' of flag tag destroyed, xhoI site at 3' of flag tag retained. XhoI in-frame with FlagDepositorTypeEmpty backboneUseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-tdPP7-3xmCherry
Plasmid#74926PurposeExpresses tdPP7-3xmCherryDepositorInsertPP7-3xmCherry
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEM4Z-T7-5'UTR-mGreenLantern-3'UTR-A64
Plasmid#203349PurposeTemplate for T7 in vitro transcription of mGreenLantern mRNA flanked by Xenopus beta globin UTRs and 64nt polyA tailDepositorInsertmGreenLantern
UseTemplate for t7-mediated in vitro transcription o…TagsFlanked by 5' and 3' UTRs from Xenopus …ExpressionMutationPromoterT7Available sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-FKBP-STING(pLxIS)
Plasmid#221333PurposeOverexpression of STING CTT in bacteriaDepositorInsertSTING CTT (Sting1 Mouse)
UseTags6xHis-4xFKBPExpressionBacterialMutationPromoterT7Available sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJFRC81-10XUAS-IVS-Syn21-GFP-p10
Plasmid#36432DepositorInsertGFP
UseTagsExpressionInsectMutationPromoterAvailable sinceMay 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
7TFC
Plasmid#24307DepositorInsert7xTcf-FFluc // SV40-mCherry
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 5, 2010AvailabilityAcademic Institutions and Nonprofits only