pJK640
(Plasmid
#72467)
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PurposeEncodes Escherichia coli evgA
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 72467 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepDRIVE-1
- Backbone size w/o insert (bp) 3851
- Total vector size (bp) 4765
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin and Kanamycin, 100 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameresponse regulator in two-component regulatory system with EvgS
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Alt nameevgA
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SpeciesEscherichia coli str. K-12 substr. MG1655
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GenBank IDNP_416870.1
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Entrez GeneevgA (a.k.a. b2369, ECK2365)
- Promoter T7
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T7 promoter
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
TA cloning product with 286 bp 5'-UTR including EvgA binding site.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJK640 was a gift from T. J. Kappock (Addgene plasmid # 72467 ; http://n2t.net/addgene:72467 ; RRID:Addgene_72467)
