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Addgene
Showing: 621 - 640 of 1302 results
  1. pDM_N1_400

    Plasmid
    #170474
    Purpose
    To amplify transcription template containing LacI operators
    Depositor
    Insert
    T7A1, O-, O1, Lambda t1 in modified lacZ fragment
    Use
    Tags
    Expression
    Bacterial
    Mutation
    diminished hairpins near O1 and diminished latent…
    Promoter
    T7A1
    Availability
    Academic Institutions and Nonprofits only
  2. pSBbi-TIR1-tTA/Pur

    Plasmid
    #171683
    Purpose
    constitutive expression of TIR1-myc and tTA in sleeping beauty transposon vector with selection marker (puromycin)
    Depositor
    Inserts
    tetracycline-controlled transactivator
    Puromycin
    EF-1a promoter
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a promoter and RPBSA promoter
    Availability
    Academic Institutions and Nonprofits only
  3. T7-TetO-GFP_ColE1

    Plasmid
    #173193
    Purpose
    Expresses GFP under the control of a T7-TetO promoter. Expression can be repressed via TetR, and repression can be relieved via addition of tetracycline. Contains the ColE1 origin of replication.
    Depositor
    Insert
    T7-TetO
    Use
    Tags
    ssrA degradation tag (DAS+4)
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
  4. pFP.R265

    Plasmid
    #138167
    Purpose
    Expresses the split mCherry reporter interrupted by the SspDnaB M86 artificially mini-intein (in cis)
    Depositor
    Inserts
    araC (araC )
    mCherryN(VDA).M86.(SDL)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    P(araBAD) and iPC
    Availability
    Academic Institutions and Nonprofits only
  5. pFP.R400

    Plasmid
    #138175
    Purpose
    Expresses the split mCherry reporter interrupted by the chimeric Npu/SspDnaE intein (in cis)
    Depositor
    Inserts
    araC (araC )
    mCherryN(GGK).NpuSsp*.(CWN)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    P(araBAD) and iPC
    Availability
    Academic Institutions and Nonprofits only
Showing: 621 - 640 of 1302 results