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pcDNA3.1-(empty)-TAG

Plasmid

#138209

Purpose

To facilitate restriction cloning of an RNA of interest with a TGT recognition element in the 3' region

Depositor

Neal Devaraj

Article

Busby et al Methods Enzymol. 2020;641:373-399. doi:

Type

Empty backbone

Use

T7 transcription

Tags

TAG sequence

Expression

Mammalian

Mutation

Promoter

CMV Promoter, T7 Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pEZY19

Plasmid

#18668

Purpose

pET19b converted Gateway Destination vector for expressing His tagged fusion proteins in bacteria. See note in comments section below regarding the mutated enterokinase site.

Depositor

Yu-Zhu Zhang

Article

Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.

Type

Empty backbone

Use

Gateway destination vector

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 26, 2008

Availability

Academic Institutions and Nonprofits only

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HLA-A2/K^b

Plasmid

#14906

Depositor

Linda Sherman

Article

Irwin et al J Exp Med. 1989 Oct 1. 170(4):1091-101.

Insert

HLA-A2/K^b (HLA-A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 15, 2007

Availability

Academic Institutions and Nonprofits only

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HLA-A2.1

Plasmid

#14904

Depositor

Linda Sherman

Article

Irwin et al J Exp Med. 1989 Oct 1. 170(4):1091-101.

Insert

HLA-A2.1 (HLA-A Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 15, 2007

Availability

Academic Institutions and Nonprofits only

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pmCherry-FKBP12-C1

Plasmid

#67900

Purpose

Expresses a fusion of mCherry with FKBP12 in mammalian cells

Depositor

Dorus Gadella

Article

van_Unen et al Scientific Reports 5, Article number: 14

Insert

mCherry-FKBP12

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pYOY152

Plasmid

#13987

Depositor

Linda Wordeman

Article

Ovechkina et al J Cell Biol. 2002 Nov 25. 159(4):557-62.

Insert

Mitotic Centromere Associated Kinesin

Use

Tags

GFP and His

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only

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pFA6a-GBP-hphMX6

Plasmid

#105159

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Davidson et al Mol Biol Cell. 2015 Feb 1;26(3):453-66.

Insert

GBP-hphMX6

Use

Tags

GBP

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Feb. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-APX(W41F)-NES

Plasmid

#42608

Depositor

Alice Ting

Article

Rhee et al Science. 2013 Jan 31.

Insert

soybean APX W41F (dimeric) -NES

Use

Tags

Flag

Expression

Mammalian

Mutation

W41F mutation

Promoter

CMV

Available since

March 20, 2013

Availability

Academic Institutions and Nonprofits only

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pSARM-luciferase

Plasmid

#67794

Purpose

Use together with VSV-G to package M-PMV reporter virus

Depositor

Stephen Goff

Article

Wang et al J Virol. 2015 Mar;89(5):2813-9. doi: 10.

Insert

firefly luciferase (gag )

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Sept. 1, 2015

Availability

Academic Institutions and Nonprofits only
pCYB-TnsA

Plasmid

#17356

Depositor

Nancy Craig

Article

Hickman et al Mol Cell. 2000 Jun . 5(6):1025-34.

Insert

TnsA (tnsA E. coli transposon Tn7)

Use

Tags

Intein-Chitin binding domain

Expression

Bacterial

Mutation

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pBS-mPlexinA2

Plasmid

#16423

Depositor

Jonathan Raper

Article

Brown et al Development. 2001 Aug . 128(16):3071-80.

Insert

Plexin A2 (Plxna2 Mouse)

Use

In situ template

Tags

Expression

Mutation

None

Promoter

Available since

Dec. 14, 2007

Availability

Academic Institutions and Nonprofits only
gS gag LV

Plasmid

#12327

Depositor

Inder Verma

Article

Kootstra et al Proc Natl Acad Sci U S A. 2003 Feb 4. 10

Insert

gag/pol

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

The CypA binding region of the WT gag was replace…

Promoter

Available since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only
T10-T7-hMOV10

Plasmid

#185052

Purpose

expression of WT human MOV10

Depositor

Constance Ciaudo

Article

Arora et al EMBO Rep. 2022 Jul 20:e54458. doi: 10.15

Insert

cDNA WT hMOV10 (MOV10 Human)

Use

Tags

T7

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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kermit/pCS2+

Plasmid

#16309

Depositor

Peter Klein

Article

Tan et al Development. 2001 Oct . 128(19):3665-74.

Insert

Kermit (gipc1 Frog)

Use

Vertebrate expression

Tags

Expression

Mutation

Promoter

Available since

Dec. 21, 2007

Availability

Academic Institutions and Nonprofits only

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gB gag LV

Plasmid

#12328

Depositor

Inder Verma

Article

Kootstra et al Proc Natl Acad Sci U S A. 2003 Feb 4. 10

Insert

gag/pol

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

The CypA binding region of the WT gag was replace…

Promoter

Available since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only
pMXS-IRES-Blast coUCK2 D62A

Plasmid

#211530

Purpose

Expresses codon optimized catalytic mutant UCK2

Depositor

Richard Possemato

Insert

UCK2 D62A (UCK2 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

D62A (catalytic mutant)

Promoter

Available since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-mCherry(Y70A)

Plasmid

#209703

Purpose

Mammalian expression of mCherry with Y70A mutation

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

mCherry(Y70A)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-HRas(G12V)

Plasmid

#209705

Purpose

Mammalian expression of HRas with G12V mutation

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

HRas (G12V)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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HPV16 E7 TCR

Plasmid

#122728

Purpose

Express HPV16 E7 TCR

Depositor

Christian Hinrichs

Article

Jin et al JCI Insight. 2018 Apr 19;3(8). pii: 9948

Insert

HPV16 E7 TCR

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

April 12, 2019

Availability

Academic Institutions and Nonprofits only

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pT2/shp53/GFP4

Plasmid

#20208

Depositor

John Ohlfest

Article

Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

Insert

shp53/GFP4

Use

Transposon

Tags

Expression

Mutation

Promoter

Available since

Dec. 3, 2009

Availability

Academic Institutions and Nonprofits only

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