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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 16423 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBluescript II KS
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 3000
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Vector typeIn Situ Template
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namePlexin A2
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Alt namePlexin 2
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SpeciesM. musculus (mouse)
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Insert Size (bp)2200
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MutationNone
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GenBank IDD86949
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Entrez GenePlxna2 (a.k.a. 2810428A13Rik, AA589422, AW457381, OCT, PlexA2, Plxn2, mKIAA0463)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byJonathan A. Raper, University of Pennsylvania
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid is an improved form of the mPlexin A2 in situ template plasmid originally described in Brown CB, Feiner L, Lu MM, Li J, Ma X, Webber AL, Jia L, Raper JA, Epstein JA. PlexinA2 and semaphorin signaling during cardiac neural crest development.
Development. 2001 128(16):3071-80. Anti-sense in situ probe is generated by linearizing with BamHI and transcribing with T3 RNA polymerase.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBS-mPlexinA2 was a gift from Jonathan Raper (Addgene plasmid # 16423 ; http://n2t.net/addgene:16423 ; RRID:Addgene_16423) -
For your References section:
PlexinA2 and semaphorin signaling during cardiac neural crest development. Brown CB, Feiner L, Lu MM, Li J, Ma X, Webber AL, Jia L, Raper JA, Epstein JA. Development. 2001 Aug . 128(16):3071-80. PubMed 11688557