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Plasmid#60439PurposePiggyBac for EOS-GFPDepositorInsertEOS
UseTagsExpressionMutationPromoterAvailable sinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
KA1775_pPBCAG-rtTAM2-IH
Plasmid#124167PurposepiggyBac-based expression of rtTA-M2DepositorInsertrtTAM2-IRES-Hph
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
vYM019_PB-Blast-pEF1a-H2B-mCh-D3bCD
Plasmid#197687Purposeplasmid for expressing the DNMT3bCDDepositorInsertH2B-mCherry-T2A-rTetR-DNMT3bCD (DNMT3B Human, Synthetic)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceApril 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
BII-ChPtW-PLAGL2
Plasmid#133380PurposePiggybac vector for constitutive expressionDepositorInsertPLAGL2 (PLAGL2 Human)
UseTagsHAExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_m4_KRAB-MeCP2_mcherry
Plasmid#167902PurposePiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmidDepositorInsertdCas9-KRAB-MeCP2
UseCRISPRTagsExpressionMutationD10A, D839A, H840A, N863APromoterAvailable sinceSept. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
KA0717_pPB-hCMV*1-cHA-IRESVenus
Plasmid#124168PurposepiggyBac-based Dox-inducible expressionDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
PB_TRE_AP4-PDGFR_EF1a_eBFP2
Plasmid#186306PurposePlasmid for inducible mammalian cell expression of the AP4 helixCAM and constitutive expression of an identifying fluorescent protein (eBFP2). Flanked with PiggyBac sites for easy line construction.DepositorInsertsAP4-PDGFR
eBFP2
UseTagsHA, mycExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
KA1065_pPB-hCMV*1-cHA-IRESH2BmCherry
Plasmid#124170PurposepiggyBac-based Dox-inducible expressionDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKDC.015
Plasmid#184992PurposeExpress -Eco2 RT and ncRNA in human cellsDepositorInsertEco2: RT and ncRNA(wt), a1/a2 length: 13
UseTagsExpressionMammalianMutationHuman codon optimized RTPromoterTetOn-3gAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAS_FLAG-Mma PylRS
Plasmid#154762Purposeplasmid with Mma PylRS for amber suppression in transient or stable, piggybac-mediated, integrationDepositorInsertMma PylRS (pylS Methanosarcina mazei)
UseTagsFLAGExpressionMammalianMutationPromoterEF1Available sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xG1 PylT sfGFP 150TAG
Plasmid#154767Purposeplasmid with 4xG1 PylT cassette and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integrationDepositorInsertsfGFP
UseTagsExpressionMammalianMutation150TAGPromoterEF1Available sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB_TRE_sg83-PDGFR_EF1a_iRFP670
Plasmid#186311PurposePlasmid for inducible mammalian cell expression of the sg83 helixCAM and constitutive expression of an identifying fluorescent protein (iRFP670). Flanked with PiggyBac sites for easy line constructionDepositorInsertssg83-PDGFR
iRFP670
UseTagsHis, mycExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB_TRE_sg88-PDGFR_EF1a_eBFP2
Plasmid#186312PurposePlasmid for inducible mammalian cell expression of the sg88 helixCAM and constitutive expression of an identifying fluorescent protein (eBFP2). Flanked with PiggyBac sites for easy line construction.DepositorInsertssg88-PDGFR
eBFP2
UseTagsHis, mycExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB_TRE_P9-PDGFR_EF1a_mPlum
Plasmid#186307PurposePlasmid for inducible mammalian cell expression of the P9 helixCAM and constitutive expression of an identifying fluorescent protein (mPlum). Flanked with PiggyBac sites for easy line construction.DepositorInsertsP9-PDGFR
mPlum
UseTagsHA, mycExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB_TRE_AP10-PDGFR_EF1a_mOrange
Plasmid#186308PurposePlasmid for inducible mammalian cell expression of the AP10 helixCAM and constitutive expression of an identifying fluorescent protein (mOrange). Flanked with PiggyBac sites for easy line constructionDepositorInsertsAP10-PDGFR
mOrange
UseTagsHA, mycExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB_TRE_P3-PDGFR_EF1a_iRFP670
Plasmid#186305PurposePlasmid for inducible mammalian cell expression of the P3 helixCAM and constitutive expression of an identifying fluorescent protein (iRFP670). Flanked with PiggyBac sites for easy line construction.DepositorInsertsP3-PDGFR
iRFP670
UseTagsHA, mycExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
BII-CPspaH2B
Plasmid#133385PurposePiggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBS-PtH
Plasmid#133391PurposePiggybac vector for SRIRACCHA-mediated mutation enrichment (donor repair template for HDR)DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
BII-Sh-PnGW
Plasmid#133359PurposePiggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLSDepositorInsertGFP-NLS
UseTagsHA-tagged GFPExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
BII-C3HTK
Plasmid#133388PurposePiggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only