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pcDNA3.1-SARS-Spike

Plasmid

#145031

Purpose

Express SARS-CoV spike protein with C9 tag at C-terminal in mammalian cells

Depositor

Fang Li

Article

Shang et al Nature. 2020 Mar 30. pii: 10.1038/s41586

Insert

SARS-CoV Spike

Use

Tags

C9 and CD5 signal peptide

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 16, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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RGS-6xHis-pcDNA3.1-

Plasmid

#52534

Purpose

enables eukaryotic expression of N-terminally RGS-6xHis–tagged fusion proteins

Depositor

Adam Antebi

Article

Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

Type

Empty backbone

Use

Tags

RGS-6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 31, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-mTagBFP2-MRTF-A

Plasmid

#182061

Purpose

Mammalian expression vector encoding myocardin-related transcription factor A tagged with mTagBFP2.

Depositor

Jin Zhang

Article

Zhang et al PLoS Biol. 2020 Sep 3;18(9):e3000866. do

Insert

mTagBFP2-MRTF-A (MRTFA Human)

Use

Tags

mTagBFP2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Clover-mRuby2

Plasmid

#49089

Purpose

Clover-mRuby2 tandem fusion for FRET calibration

Depositor

Kurt Beam

Insert

Clover-mRuby2 fusion

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 3xF-GAR1

Plasmid

#126873

Purpose

Expresses 3xFlag GAR1

Depositor

Kathleen Collins

Article

Egan et al Mol Cell Biol. 2010 Jun;30(11):2775-86.

Insert

GAR1 (GAR1 Human)

Use

Tags

3xFlag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 CLN3-GFP

Plasmid

#78110

Purpose

Mammalian expression of human CLN3 with C terminal GFP fusion

Depositor

Thomas Braulke

Insert

CLN3 (CLN3 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 20, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-Flag-RBM24

Plasmid

#208052

Purpose

Expressing N-ter Flag tagged human RBM24 (ENST00000379052) in mammalian cells

Depositor

Quanfu Ma

Insert

human RBM24 (ENST00000379052) coding region (RBM24 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsDIS3_Z

Plasmid

#148111

Purpose

Mammalian Expression of HsDIS3

Depositor

Elisa Izaurralde

Insert

HsDIS3 (DIS3 Human)

Use

Tags

Expression

Mammalian

Mutation

L760F compared to the sequence given by isoform 2…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_iSH2-pMag(3x)-iRFP

Plasmid

#67304

Purpose

Blue light dependent photoswitching system

Depositor

Moritoshi Sato

Article

Kawano et al Nat Commun. 2015 Feb 24;6:6256. doi: 10.

Insert

iSH2-pMag(3x)-iRFP (PIK3R1 Human, Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+/mit-2mutAEQ

Plasmid

#45539

Depositor

Javier Alvarez-Martin

Article

de la Fuente et al Biochem J. 2012 Aug 1;445(3):371-6. doi:

Insert

mit-2mutAEQ

Use

Tags

Mitochondrially targeted

Expression

Mammalian

Mutation

Asp119Ala and Asn28Leu

Promoter

Available since

July 3, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 SARS-CoV-2 M

Plasmid

#158078

Purpose

Expresses SARS-CoV-2 membrane protein in mammalian cells

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Insert

SARS-CoV-2 matrix (M )

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 3xFlag IFIT3

Plasmid

#53553

Purpose

mammalian expression of IFIT3

Depositor

Kathleen Collins

Article

Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

Insert

IFIT3 (IFIT3 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 29, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-GGamma7-GFP2

Plasmid

#166778

Purpose

Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

Depositor

Bryan Roth

Article

Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

Insert

GGamma7-GFP2 (GNG7 Human, Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Contains an N-terminal GFP2 and GSAG linker

Promoter

CMV

Available since

April 27, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 SARS-CoV-2 E

Plasmid

#158080

Purpose

Expresses SARS-CoV-2 envelope protein in mammalian cells

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Insert

SARS-CoV-2 envelope (E )

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 15, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-DDR2-V5/HIS

Plasmid

#201101

Purpose

expression of human DDR2 receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

human DDR2 receptor tyrosine kinase, full length, wildtype (DDR2 Human)

Use

Tags

V5/His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-) FLIPQTV3.0 100micro

Plasmid

#63730

Purpose

FLIPQTV3.0 glutamine sensor with D157N mutation

Depositor

Sakiko Okumoto

Article

Gruenwald et al PLoS One. 2012;7(6):e38591. doi: 10.1371

Insert

FLIPQTV3.0 sensor, 100microM affinity

Use

Tags

Expression

Mammalian

Mutation

D157N in glutamine binding domain

Promoter

Available since

Nov. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FLT3-V5/HIS

Plasmid

#201985

Purpose

expression of human FLT3 receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

FLT3 receptor tyrosine kinase (FLT3 Human)

Use

Tags

V5, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 21, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)- e18 LOF

Plasmid

#131729

Purpose

Expresses a loss of function mutant of an engineered variant of RAD18 ("e18")

Depositor

Alberto Ciccia

Article

Nambiar et al Nat Commun. 2019 Jul 30;10(1):3395. doi:

Insert

e18/D221A (Loss of function mutant of RAD18 with SAP domain deleted and UBZ point mutation) (RAD18 Human)

Use

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-Flag-H2A

Plasmid

#63560

Purpose

Expression of human H2A tagged with Flag

Depositor

Titia Sixma

Article

Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

Insert

H2A (H2AC13 Human)

Use

Tags

Flag (MDYKDDDDKVPRIQ)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 3xFlag IFIT1

Plasmid

#53554

Purpose

mammalian expression of IFIT1

Depositor

Kathleen Collins

Article

Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

Insert

IFIT1 (IFIT1 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 29, 2014

Availability

Academic Institutions and Nonprofits only

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