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Plasmid#168019PurposeExpresses X(5010)B1-6xHis in bacterial cellsDepositorInsertX(5010)B1-6xHis
UseTagsHisx6ExpressionBacterialMutationPromoterAvailable sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET26b-Granzyme A
Plasmid#8823DepositorInsertHuman Granzyme A (GZMA Human)
UseTags(His)6 and Enterokinase siteExpressionBacterialMutationit mature enzyme.PromoterAvailable sinceSept. 26, 2005AvailabilityAcademic Institutions and Nonprofits only -
pET21-Flavidin
Plasmid#89881PurposeFluorophore-friendly streptavidin that can improve fluorescent brightness and has unimpaired biotin binding after dye labelingDepositorInsertStreptavidin-K80R-K121R-K132R-K134R-Glutamate_Tag
UseTags6 glutamate tag (C terminal on insert)ExpressionBacterialMutationK80R, K121R, K132R, K134RPromoterAvailable sinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28-Kss1
Plasmid#52682PurposeExpression of Kss1 in bacteriaDepositorInsertKSS1 (KSS1 Budding Yeast)
UseTagsHis6 and T7 tagExpressionBacterialMutationPromoterT7Available sinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pETM-11-HsPABPC1_J
Plasmid#146642PurposeBacterial Expression of HsPABPC1DepositorInsertHsPABPC1 (PABPC1 Human)
UseTagsExpressionBacterialMutationtwo silent mutations compared to the sequence giv…PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-SUMO-A'-l4-G'
Plasmid#209124PurposeBacterial expression of CC-GEMS ligand SUMO-A'-l4-G'DepositorInsertSUMO-A'-l4-G'
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET24a MDH_9CREN
Plasmid#204243PurposeBacterial expression of I. islandicus malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).InsertMDH_9CREN
UseTags6X HisExpressionBacterialMutationPromoterLacAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-53-RtcB
Plasmid#51282PurposeE. coli expression vector for E. coli RtcB RNA ligaseDepositorInsertE. coli RtcB
UseTags6xHisExpressionBacterialMutationPromoterT7 RNA PolymeraseAvailable sinceMarch 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a-PFO
Plasmid#92109PurposeRecombinant expression in E.ColiDepositorInsertPFO
UseTags6xHisExpressionBacterialMutationDelta27aaPromoterAvailable sinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET31b-T7-Spinach2
Plasmid#79783PurposeExpresses Spinach2 under a T7 promoterDepositorInsertT7-tRNA-Spinach2
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET32a-TRXtag
Plasmid#11516DepositorInsertNone - thioredoxin is vector encoded (trxA )
UseTagsHis, His (out of frame), and TrxExpressionBacterialMutationvector modified; removed N-terminal His tag by di…PromoterAvailable sinceApril 28, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET-Galectin-8 FL
Plasmid#68805PurposeExpress Galectin-8 FL protein in Bacterial cellsDepositorInsertGalectin-8 (LGALS8 Human)
UseTagsN-terminal 6-histidine-tagExpressionBacterialMutationNo mutationPromoterT7Available sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a MISTIC
Plasmid#85999Purposebacterial expression of transmembrane proteins using N-terminal MISTIC and C-terminal HIS6 tagDepositorInsertN/A
UseTagsHIS6 and MISTICExpressionBacterialMutationcodon optimzed for bacterial expressionPromoterT7Available sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
NylB'-SCY_pET21b
Plasmid#215417PurposeExpression construct for NylB'-SCY gene, codon optimized for expression in E. coliDepositorInsertNylB'-SCY
UseTagsExpressionBacterialMutationR187S/F264C/D370YPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET12a-PAPI
Plasmid#19961DepositorInsertAnnexin V (ANXA5 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 16, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET-IG-mSA
Plasmid#80706PurposeExpresses monomeric streptavidin (mSA) in bacterial cellsDepositorInsertMonomeric streptavidin
UseTags10xHis tag and TEV siteExpressionBacterialMutationPromoterT7Available sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET29a-hNABP1
Plasmid#128306PurposeExpress hNABP1 (a.k.a hSSB2 or SOSSB2) in E. coli with a C-terminal His-tagDepositorInsertHuman nucleic acid-binding protein 1 (hNABP1) (NABP1 Human)
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
Dapp1-PH pET11a
Plasmid#71376PurposePhosphoinositide BindingDepositorInsertDapp1-PH (DAPP1 Human)
UseTagsExpressionBacterialMutationPleckstrin homology (PH) domain; aa 148-273PromoterT7Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET15-UbE2W
Plasmid#15809DepositorInsertUbiquitin conjugating enzyme UbE2W (UBE2W Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET28b-BE1
Plasmid#73018PurposeExpresses BE1 with N-terminal His6 tag in E. ColiDepositorInsertBE1
UseTagsHis6 tagExpressionBacterialMutationPromoterT7Available sinceApril 14, 2016AvailabilityAcademic Institutions and Nonprofits only