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Plasmid#194558PurposeMammalian Expression of mEGFP-HMGB1 Mutant (K184Rfs*44) Fusion Protein, "R or K > A" variantDepositorInsertHMGB1 (HMGB1 Human)
UseTagsmEGFPExpressionMammalianMutationR or K > A substitutions after Lys185PromoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5-mEGFP-HMGB1-MUT-RtoA
Plasmid#194557PurposeMammalian Expression of mEGFP-HMGB1 Mutant (K184Rfs*44) Fusion Protein, "R > A" variantDepositorInsertHMGB1 (HMGB1 Human)
UseTagsmEGFPExpressionMammalianMutationR > A substitutions after Lys185PromoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5-mEGFP-HMGB1-MUT-Patchless
Plasmid#194553PurposeMammalian Expression of mEGFP-HMGB1 Mutant (K184Rfs*44) Fusion Protein, "Patchless" variantDepositorInsertHMGB1 (HMGB1 Human)
UseTagsmEGFPExpressionMammalianMutationK184Rfs*44, truncation by stop codon after Lys209PromoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-fDIO-mEGFP-Gphn_P1
Plasmid#194978PurposeAAV vector to drive the Flp-dependent expression of mEGFP (L221K) -Gphn (isoform P1) under the control of human Synapsin promoterDepositorInsertmEGFP-Gphn (isoform P1) (Gphn Rat, Synthetic)
UseAAVTagsmEGFPExpressionMutationPromoterAvailable sinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmCherry2-CD86-mEYFP(Q69K)-mEGFP
Plasmid#190751PurposeExpression of triple tagged CD86 with mCherry2, mEYFP(Q69K), and mEGFP in mammalian cells.DepositorInsertCD86 (CD86 Human)
UseTagsmCherry2, mEGFP, and mEYFP(Q69K)ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
myr-palm-mEYFP-(L)-mCherry2-(L)-mEGFP
Plasmid#182853PurposeHetero-trimer expression vector with plasma membrane localization signalDepositorInsertmyr-palm-mEYFP-mCherry2-mEGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
C232-E05: attB2r-meGFP-attB3
Plasmid#162902PurposeGateway attB2r/attB3 entry clone for construction of C-terminal eGFP fusion proteinsDepositorInsertmonomeric enhanced Green fluorescent protein
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_shRNA resistant PKA-Cα(E12,14K)-mEGFP
Plasmid#168491PurposeMammalian expression of the E12,14K mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric EGFP. Silent mutations were introduced to make it resistant to shRNA knock-down.DepositorInsertthe E12,14K mutant of the mouse PKA catalytic subunit α C-terminally tagged by mEGFP. Resistant to shRNA knock-down.
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
CaMKIIP-mEGFP-P2A-paAIP2 (R5AR6A)/pAAV
Plasmid#91719PurposeAAV-mediated expression of mEGFP and paAIP2 (R5AR6A), the functional mutant of paAIP2DepositorInsertmEGFP-P2A-paAIP2(R5AR6A)
UseAAVTagsExpressionMutationPromoterCaMKII 1.3Available sinceJune 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSems-leader-SNAPf-mEGFPe-IFNAR1(28-557)
Plasmid#187844PurposeExpression of SNAPf and mEGFPe-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopyDepositorInsertIFNAR1 (IFNAR1 Human)
UseTagsIg k-chain leader sequence, SNAPf-tag, and meGFPe…ExpressionMammalianMutationmeGFPe: asparagine 198 to aspartic acid and tyros…PromoterCMVAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mEGFP-CaMKIIa (T286D/T305A/T306A)
Plasmid#127392PurposeFluorescent reporter for mutant Ca2+/calmodulin-dependent protein kinase II alpha (T286D/T305A/T306A)DepositorInsertcalcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)
UseTagsmEGFPExpressionMammalianMutationchanged Threonine 286 to Aspartic acid and Threon…PromoterpCAGAvailable sinceJuly 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1alpha-nCoV2019-N-meGFP-IRES-Puro
Plasmid#185451PurposeExpresses SARS-CoV-2 nucleocapsid protein with a c-terminal monomeric EGFP in mammalian cellsDepositorInsertNucleocapsid (N SARS-CoV-2)
UseLentiviralTagsmEGFPExpressionMammalianMutationPromoterEF1alphaAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRK5-mEGFP-HMGB1-MUT-Full-length
Plasmid#194550PurposeMammalian Expression of mEGFP-HMGB1 Mutant (K184Rfs*44) Fusion ProteinDepositorInsertHMGB1 (HMGB1 Human)
UseTagsmEGFPExpressionMammalianMutationK184Rfs*44PromoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
CMV-mEGFP(A206K)-paCaMKII(K42M)-ShadowG
Plasmid#167477PurposeExpresses paCaMKII in mammalian cells to measure structural change of paCaMKII.DepositorInsertmEGFP(A206K)-paCaMKII(K42M)-ShadowG
UseTagsShadowG and mEGFP(A206K)ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-mEGFP(A206K)-paCaMKII(T286A)-ShadowG
Plasmid#167476PurposeExpresses paCaMKII in mammalian cells to measure structural change of paCaMKII.DepositorInsertmEGFP(A206K)-paCaMKII(T286A)-ShadowG
UseTagsShadowG and mEGFP(A206K)ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-mEGFP-loxP-P2A-dTKneo-pA-loxP
Plasmid#139484PurposePCR template for mEGFP gene knock-in donor vectorDepositorInsertmEGFP-loxP-P2A-dTKneo-pA-loxP
UseTagsmEGFPExpressionMammalianMutationPromoterCAG promoterAvailable sinceApril 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-FLEX-tmeGFP-IRES-nls-mRuby2
Plasmid#63060PurposeExpresses truncated monomeric eGFP and nuclear localized mRuby2 (linked with IRES) in a Cre-dependent manner in an AAV backboneDepositorInsertnls-mRuby2
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterCAGAvailable sinceJune 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPB-TetON-mEGFP-HMGB1-MUT-Patchless
Plasmid#194563PurposeDox-inducible Mammalian Expression of mEGFP-HMGB1 Mutant "Patchless" (K184Rfs*44, "Patchless" variant) Fusion ProteinDepositorInsertHMGB1 (HMGB1 Human)
UseTagsmEGFPExpressionMammalianMutationK184Rfs*44, truncation by stop codon after Lys209PromoterAvailable sinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWPT-ACC/ACC-mEGFP-IRES-mCherry
Plasmid#49229PurposeLentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.DepositorInsertsmEGFP
mCherry
UseLentiviral and Synthetic BiologyTagsSfuI site to create C-terminal fusionsExpressionMammalianMutationPromoterEF1-shortAvailable sinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pWPT-UCU/ACC-mEGFP-IRES-mCherry
Plasmid#49232PurposeLentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.DepositorInsertsmEGFP
mCherry
UseLentiviral and Synthetic BiologyTagsSfuI site to create C-terminal fusionsExpressionMammalianMutationPromoterEF1-shortAvailable sinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only