-
Plasmid#39283DepositorInsertnmt1 promoter (nmt1 Fission Yeast)
UseYeast genomic targetingTags3xHA, A. gossypii translation elongation factor 1…ExpressionMutation-1163 to +6 of nmt1 locusPromoterAvailable sinceAug. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoTagRFP657-Kan
Plasmid#44955PurposeYeast tagging vector to create gene fusion with yeast optimized TagRFp657 with Kan selectionDepositorInsertyoTagRFP657
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUC-H1-Esp3I-mU6-Kan
Plasmid#213015PurposeVector for Cloning of multiple gRNAs driven by distinct promoters (H1 and mU6)DepositorInsertH1 promoter, mU6 promoter, gRNA scaffolds
UseTagsExpressionMutationPromoterH1 promoterAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yomRuby-Kan
Plasmid#44956PurposeYeast tagging vector to create gene fusion with yeast optimized mRuby with Kan selectionDepositorInsertyomRuby
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUC19-link-sfGFP-FLAG:Kan
Plasmid#211833PurposePlasmid template that encodes the fluorescent protein sfGFP with a FLAG tag next to kanamycin resistance gene. This template was used to copy out sfGFP-FLAG-Kan for genetic tagging.DepositorInsertsfGFP-FLAG-Kan
UseTagsExpressionBacterialMutationNAPromoterAvailable sinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC19-link-PAGFP-FLAG:Kan
Plasmid#211834PurposePlasmid template that encodes the fluorescent protein PAGFP with a FLAG tag next to kanamycin resistance gene. This template was used to copy out PAGFP-FLAG-Kan for genetic tagging.DepositorInsertPAGFP-FLAG-Kan
UseTagsExpressionBacterialMutationNAPromoterAvailable sinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-PGAL1-GFP
Plasmid#41614DepositorInsertGAL1 promoter (GAL1 Budding Yeast)
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…ExpressionMutationPromoterAvailable sinceDec. 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P81nmt1-mYFP
Plasmid#105144PurposeYeast gene targetingDepositorInsertkanMX6-P81nmt1-mYFP
UseTags81nmt1-mYFPExpressionBacterial and YeastMutationA206KPromoter81nmt1Available sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P3nmt1-GFP
Plasmid#39289DepositorInsertnmt1 promoter (nmt1 Fission Yeast)
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…ExpressionMutation-1163 to +6 of nmt1 locusPromoterAvailable sinceSept. 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDZ251 (pKAN 24xPP7SL cyc)
Plasmid#35192DepositorInsert24xPP7SL cyc
UseTagsExpressionMutationPromoterAvailable sinceFeb. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pKanCMV-CX4.3-7aa-mClover3
Plasmid#74260PurposeMammalian expression of C-terminal fusion of mClover3 to CX43DepositorInsertConnexin 43
UseTagsmClover3ExpressionMammalianMutationPromoterCAG and CMVAvailable sinceMarch 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pETNKI-his-3C-LIC-kan
Plasmid#108703PurposeExpression of an His-tagged target protein with 3C protease cleavage site.DepositorTypeEmpty backboneUseTagsHis-3C protease cleavage siteExpressionBacterialMutationPromoterT7Available sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGZ173 (pFA6a-SL-MNase-kanMX6)
Plasmid#70234PurposeChEC-tagging vector encoding a short linker and aa 83-231 of Mnase in pFA6a-3HA-kanMX6 replacing the 3xHA tag. These vectors retain compatibility with the F2/R1 primer pairs commonly used for epitopeDepositorInsertShort linker and aa 83-231 of Mnase
UseYeast genomic targetingTagsExpressionMutationPromoterAvailable sinceNov. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P41nmt-3HA
Plasmid#39284DepositorInsertnmt1 promoter (nmt1 Fission Yeast)
UseYeast genomic targetingTags3xHA, A. gossypii translation elongation factor 1…ExpressionMutation-1163 to +6 of nmt1 locus with a 4bp deletion (TA…PromoterAvailable sinceAug. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDonor14-ShoHELIX-KanR (BO4)
Plasmid#181795PurposepDonor for ShoHELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequence and encoding a kanamycin resistance gene as cargoDepositorInsertKanR, R6K origin of replication
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P41nmt1-GFP
Plasmid#39290DepositorInsertnmt1 promoter (nmt1 Fission Yeast)
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…ExpressionMutation-1163 to +6 of nmt1 locus with a 4bp deletion (TA…PromoterAvailable sinceAug. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P81nmt1-3HA
Plasmid#39285DepositorInsertnmt1 promoter (nmt1 Fission Yeast)
UseYeast genomic targetingTags3xHA, A. gossypii translation elongation factor 1…ExpressionMutation-1163 to +6 of nmt1 locus with a 7bp deletion (AT…PromoterAvailable sinceAug. 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoClover-Kan
Plasmid#44952PurposeYeast tagging vector to create gene fusion with yeast optimized Clover with Kan selectionDepositorInsertyoClover
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-G11-5FLAG-kanMX6
Plasmid#49182Purposegenomic G11-5FLAG epitope tagging in S. pombeDepositorTypeEmpty backboneUseTagsG11-5FLAGExpressionYeastMutationPromoterAvailable sinceNov. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -