-
Plasmid#72230PurposeUracil selection; Expression of PP7-2x-yeGFP fusion protein under the MET25 promoterDepositorInsertPP7-PS-2x-yeGFP
UseTagsExpressionYeastMutationPromoterMET25Available sinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDZ536 pURA ADE3p PP7-PS-2x-yeGFP
Plasmid#72234PurposeUracil selection; Expression of PP7-2x-yeGFP fusion protein under the ADE3 promoterDepositorInsertPP7-PS-2x-yeGFP
UseTagsExpressionYeastMutationPromoterADE3pAvailable sinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTAL3
Plasmid#31034DepositorInsertTALEN-FokI nuclease backbone
UseTagsAcV5 and FokI nucleaseExpressionMutationPromoterAvailable sinceJune 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
GPKAnls
Plasmid#61094PurposeGFP-tagged PKA catalytic subunit targeted to the nucleus by addition of a nuclear localization signalDepositorInsertpkac2
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
GPKAnes
Plasmid#61092PurposeGFP-tagged PKA catalytic subunit targeted to the extranuclear cytoplasm by addition of a nuclear export signalDepositorInsertpkac2
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTAL4
Plasmid#31035DepositorInsertTALEN-FokI nuclease backbone
UseTagsAcV5 and FokI nucleaseExpressionMutationPromoterAvailable sinceJune 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HIRAmut-polyA
Plasmid#59782PurposeThis Hira gene contains silent mutations in siRNA-targeted sequences (see Inoue and Zhang, 2014 NSMB).DepositorInsertHIRA
UseTagsExpressionMammalianMutationChange from 'GATCGAAGTTTG' to 'GAc…PromoterCMV and T7Available sinceSept. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJK571
Plasmid#75379PurposeProduces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase subunits and associated hypothetical protein with N-terminal His6 tag (AaPurS, H6AaOrfY, AaPurQ, AaPurL-G209D)DepositorInsertsformylglycinamidine ribonucleotide synthetase, small subunit
hypothetical protein in purSQL operon
formylglycinamidine ribonucleotide synthetase, glutaminase subunit
formylglycinamidine ribonucleotide synthetase, large subunit
UseGene synthesisTagsHis6ExpressionBacterialMutationchanges glycine-209 to aspartate and synthetic ge…Promotern/aAvailable sinceAug. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDZ529 pTRP MET25p PP7-PS-2x-yeGFP
Plasmid#72233PurposeTryptophan selection; Expression of PP7-2x-yeGFP fusion protein under the MET25 promoterDepositorInsertPP7-PS-2x-yeGFP
UseTagsExpressionYeastMutationPromoterMET25Available sinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pC2.4
Plasmid#226557PurposeNATURA gene encoding plasmidDepositorInsertNATURA reporter gene
UseLuciferase; PiggybacTagsEGFP and FlagExpressionMammalianMutationPromoterEF1alphaAvailable sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFastBac WT SOX
Plasmid#192055PurposeExpression vector for WT SOXDepositorInsertWT SOX (ORF37)
UseTagsExpressionInsectMutationPromoterPolyhedrinAvailable sinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-T7-OmpA-MNaseA-6xHis
Plasmid#214808PurposeExpression of 6xhis-tagged micrococcal nuclease A (MNaseA)DepositorInsertOmpA-Micrococcal nuclease A
UseTags6xhisExpressionBacterialMutationPromoterT7 promoterAvailable sinceNov. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-T7-OmpA-MNaseB-6xHis
Plasmid#214809PurposeExpression of 6xhis-tagged micrococcal nuclease B (MNaseB)DepositorInsertOmpA-Micrococcal nuclease B
UseTags6xhisExpressionBacterialMutationPromoterT7 promoterAvailable sinceNov. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS2696-CMV-Nme2SmuCas9-D56R
Plasmid#228717PurposeExpresses Nme2Smu Cas9 nuclease with D56R mutation in mammalian cellsDepositorInsertNme2SmuCas9-D56R nuclease
UseCRISPRTagsExpressionMammalianMutationNme2-Cas9, Smu PID, D56RPromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS2697-CMV-Nme2SmuCas9-E932R
Plasmid#228718PurposeExpresses Nme2Smu Cas9 nuclease with E932R mutation in mammalian cellsDepositorInsertNme2SmuCas9-E932R nuclease
UseCRISPRTagsExpressionMammalianMutationNme2-Cas9, Smu PID, E932RPromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKJ248
Plasmid#217494PurposeMammlian expression of DpFnuc guide RNADepositorInsertDpFnuc guide RNA scaffold
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET3a-Xl-H3∆Nterm[R42]
Plasmid#221201PurposeXenopus laevis histone H3 with N-terminal tail deleted.DepositorInsertXl-H3∆Nterm[R42]
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKJ223
Plasmid#217497PurposeBacterial expression of BaFnuc guide RNADepositorInsertBaFnuc guide RNA scaffold
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKJ224
Plasmid#217496PurposeBacterial expression of BaFnuc for IVTTDepositorInsertBaFnuc
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKJ235
Plasmid#217493PurposeMammlian expression of MmFnuc for genome editingDepositorInsertMmFnuc
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only