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pcDNA3-FluoSTEP-AKAR (C/Y)

Plasmid

#181862

Purpose

FluoSTEP-AKAR using split CFP and cpVenus(E172) as the FRET donor and acceptor. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

Depositor

Jin Zhang

Article

Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

Insert

FluoSTEP-AKAR (C-Y)

Use

Tags

CFP(1-10) and cpVenus(E127)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pDH18

Plasmid

#83803

Purpose

YFP-CFP positive FRET control; his selection

Depositor

Eric Muller

Article

Hailey et al Methods Enzymol. 2002;351:34-49.

Insert

YFP-CFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pBS5

Plasmid

#83799

Purpose

Useful for tagging yeast proteins with N-terminal CFP tag; G418 selection

Depositor

Eric Muller

Article

Hailey et al Methods Enzymol. 2002;351:34-49.

Insert

CFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pHL 1701

Plasmid

#69788

Purpose

KanR with FRT sites + pT7::T710::CFP::T7 terminator::T1 terminator + ColE

Depositor

Han Lim

Article

Shin et al Nucleic Acids Res. 2016 Apr 15. pii: gkw

Insert

CFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
CKAR

Plasmid

#14860

Depositor

Alexandra Newton

Article

Violin et al J Cell Biol. 2003 Jun 9. 161(5):899-909.

Insert

C kinase activity reporter

Use

Tags

CFP and YFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3-S28 histone 3 phosphorylation reporter-NLS

Plasmid

#32787

Depositor

Alice Ting

Article

Lin et al Angew Chem Int Ed Engl. 2004 May 24;43(2

Insert

CFP-14-3-3tau-H3 peptide-YFP

Use

Tags

CFP and YFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 7, 2011

Availability

Academic Institutions and Nonprofits only
pRSETB-S28 histone 3 phosphorylation reporter

Plasmid

#32788

Depositor

Alice Ting

Article

Lin et al Angew Chem Int Ed Engl. 2004 May 24;43(2

Insert

CFP-14-3-3tau-H3 peptide-YFP

Use

Tags

CFP and YFP

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 16, 2011

Availability

Academic Institutions and Nonprofits only
pBS Coupler 1

Plasmid

#185824

Purpose

Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3].

Depositor

Atsushi Miyawaki

Article

Shimozono et al Methods Cell Biol. 2008;85:381-93. doi:

Insert

(GlyGlyGlyGlySer)x3 linker

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pBS Coupler 6

Plasmid

#185829

Purpose

Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3].

Depositor

Atsushi Miyawaki

Article

Shimozono et al Methods Cell Biol. 2008;85:381-93. doi:

Insert

(GlyGlyGlyGlySer)x3 linker

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS Coupler 5

Plasmid

#185828

Purpose

Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3].

Depositor

Atsushi Miyawaki

Article

Shimozono et al Methods Cell Biol. 2008;85:381-93. doi:

Insert

(GlyGlyGlyGlySer)x3 linker

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS Coupler 4

Plasmid

#185827

Purpose

Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3].

Depositor

Atsushi Miyawaki

Article

Shimozono et al Methods Cell Biol. 2008;85:381-93. doi:

Insert

(GlyGlyGlyGlySer)x3 linker

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS Coupler 2

Plasmid

#185825

Purpose

Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3].

Depositor

Atsushi Miyawaki

Article

Shimozono et al Methods Cell Biol. 2008;85:381-93. doi:

Insert

(GlyGlyGlyGlySer)x3 linker

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS Coupler 3

Plasmid

#185826

Purpose

Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3].

Depositor

Atsushi Miyawaki

Article

Shimozono et al Methods Cell Biol. 2008;85:381-93. doi:

Insert

(GlyGlyGlyGlySer)x3 linker

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
OA-874PA

Plasmid

#164846

Purpose

Expresses AAEL005635-Cas9 with OpIE2-CFP marker

Depositor

Omar Akbari

Article

Li et al Nat Commun. 2021 Sep 10;12(1):5374. doi:

Insert

P10-3'UTR OpIE2-CFP

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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MyrPalm-CKAR

Plasmid

#14862

Depositor

Alexandra Newton

Article

Violin et al J Cell Biol. 2003 Jun 9. 161(5):899-909.

Insert

C kinase activity reporter

Use

Tags

CFP, Myr/Palm, and YFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only

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pSB233

Plasmid

#164128

Purpose

PP7-CFP and PhlF-RFP expression plasmid

Depositor

Christopher Voigt

Article

Shao et al Nat Commun. 2021 Mar 5;12(1):1475. doi:

Insert

PP7-CFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Ptet

Available since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pTH102

Plasmid

#84460

Purpose

pJB38-NWMN29-30 + SarA_P1-CFP-Term

Depositor

Reindert Nijland

Article

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Insert

SarA_P1-CFP from pTH2

Use

Tags

Expression

Mutation

Promoter

Available since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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CytoCKAR

Plasmid

#14870

Depositor

Alexandra Newton

Article

Gallegos et al J Biol Chem. 2006 Oct 13. 281(41):30947-

Insert

C kinase activity reporter

Use

Tags

CFP, NES, and YFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only
CKAR-T/A

Plasmid

#14861

Depositor

Alexandra Newton

Article

Violin et al J Cell Biol. 2003 Jun 9. 161(5):899-909.

Insert

C kinase activity reporter

Use

Tags

CFP and YFP

Expression

Mammalian

Mutation

T/A

Promoter

Available since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
MitoCKAR

Plasmid

#14872

Depositor

Alexandra Newton

Article

Gallegos et al J Biol Chem. 2006 Oct 13. 281(41):30947-

Insert

C kinase activity reporter

Use

Tags

CFP, TOM20(1-33), and YFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only