We narrowed to 672 results for: Bacillus

1. pYPQ292-D977A

   Plasmid

   #129680

   Purpose

   Gateway entry clone for CRISPR-Cas12b systems

   Depositor

   Yiping Qi

   Article

   Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

   Insert

   AaCas12b
   

   Use

   CRISPR; Gateway compatible aacas12b entry clone

   Expression

   Plant

   Mutation

   D977A; AaCas12b is rice codon optimized

   Available Since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYPQ292-E848A

   Plasmid

   #129681

   Purpose

   Gateway entry clone for CRISPR-Cas12b systems

   Depositor

   Yiping Qi

   Article

   Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

   Insert

   AaCas12b
   

   Use

   CRISPR; Gateway compatible aacas12b entry clone

   Expression

   Plant

   Mutation

   E848A; AaCas12b is rice codon optimized

   Available Since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFA-dabAB2

   Plasmid

   #133012

   Purpose

   pFA carrying the badabA2 (locus_tag: BAS2958) and badabB2 genes (locus_tag: BAS2959)

   Depositor

   David Savage

   Article

   Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

   Inserts

   dabB2
   dabA2
   

   Expression

   Bacterial

   Promoter

   tetR

   Available Since

   Nov. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFE-dabAB2 C351A

   Plasmid

   #133005

   Purpose

   pFE carrying a dabA2 gene with a C351A mutation and a dabB2 gene (Uniprot: D0KWS8)

   Depositor

   David Savage

   Article

   Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

   Inserts

   dabB2
   dabA2
   

   Expression

   Bacterial

   Mutation

   C351A

   Promoter

   tetR

   Available Since

   Nov. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKAR2-Br512_Mut235

   Plasmid

   #179278

   Purpose

   Expresses Br512_Mut235 polymerase in E.coli

   Depositor

   Andrew Ellington

   Article

   Paik et al Biochemistry. 2021 Nov 11. doi: 10.1021/

   Insert

   Engineered Bst DNA Polymerase I Large Fragment
   

   Tags

   8x His tag; Villin Headpiece 47 amino acid (HP47)…

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCRISPR-PsppA-mCherry

   Plasmid

   #225428

   Purpose

   Plasmid encodes SgRNA, SpCas9 and homologous arms for homologous recombination of mCherry

   Depositor

   Geir Mathiesen

   Article

   Wiull et al Microbiol Spectr. 2025 Feb 4;13(2):e0202

   Insert

   mCherry, Cas9, SgRNA
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   PsppA upstream of mCherry and Cas9, P3 upstream o…

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCB578

   Plasmid

   #141095

   Purpose

   E. coli-Lactobacilli shuttle vector containing SpCas9, tracrRNA, and a repeat-spacer-repeat array

   Depositor

   Chase Beisel

   Article

   Leenay et al Biotechnol J. 2019 Mar;14(3):e1700583. d

   Inserts

   Cas9 and tracrRNA
   repeat-spacer-repeat array
   

   Expression

   Bacterial

   Available Since

   April 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVX-TetOne-Puro-mitoLbNOX-FLAG

   Plasmid

   #218667

   Purpose

   pLVX-TetOne-Puro-mitoLbNOX-FLAG

   Depositor

   Valentin Cracan

   Article

   Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038

   Insert

   mitoLbNOX
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   FLAG and MTS

   Expression

   Mammalian

   Available Since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLVX-TetOne-Puro-LbNOX-FLAG

   Plasmid

   #218631

   Purpose

   This plasmid contains LbNOX under doxycycline inducible control.

   Depositor

   Valentin Cracan

   Article

   Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038

   Insert

   LbNOX
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLPP6

    Plasmid

    #209968

    Purpose

    Contains Level 0 Part: Promoter Dropout (sfGFP) for the construction of Level 1 plasmids and subsequent promoter insertion

    Depositor

    Thorsten Mascher

    Article

    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

    Insert

    Promoter Dropout (sfGFP)
    

    Expression

    Bacterial

    Available Since

    Sept. 13, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pMZ7

    Plasmid

    #223180

    Purpose

    pORI28 derivative with lacZ and upp gene inserted into the backbone; for generating gene-specific knockouts.

    Depositor

    Paul Blainey

    Article

    Zhu et al bioRxiv [Preprint]. 2023 Dec 30:2023.12.

    Type

    Empty backbone

    Use

    Chromosomal integration

    Available Since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFA-DAB2

    Plasmid

    #162690

    Purpose

    Expresses DabAB2 inorganic carbon transport complex

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    DabAB2 inorganic carbon transport complex
    

    Expression

    Bacterial

    Promoter

    PLtet0-1 promoter

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a-ybbR-HIS-Xyl-DocI

    Plasmid

    #58711

    Purpose

    Characteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tag

    Depositor

    Hermann Gaub

    Article

    Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

    Insert

    Xyl-DocI
    

    Tags

    HIS, HRV 3C, and ybbR

    Expression

    Bacterial

    Mutation

    changed Threonin 120 to Cysteine in Xylanase Doma…

    Promoter

    T7

    Available Since

    Aug. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pZ005 (AacC2c1 human codon optimized protein)

    Plasmid

    #70171

    Purpose

    Expresses AacC2c1 human codon optimized protein

    Depositor

    Feng Zhang

    Article

    Shmakov et al Mol Cell. 2015 Nov 5;60(3):385-97. doi:

    Insert

    AacC2c1 protein
    

    Tags

    NLS

    Available Since

    May 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYPQ292 (AaCas12b)-D570A-TV

    Plasmid

    #136380

    Purpose

    CRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Insert

    AaCas12b (D570A)-TV
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    D570A

    Available Since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYPQ292-D570A-SRDX

    Plasmid

    #129684

    Purpose

    Gateway entry clone for CRISPR-Cas12b systems

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Insert

    AaCas12b-SRDX
    

    Use

    CRISPR; Gateway compatible aacas12b entry clone

    Expression

    Plant

    Mutation

    D570A; AaCas12b is rice codon optimized

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLz74_Rubisco_CsoS2_Npeptide_fusion

    Plasmid

    #140840

    Purpose

    Same as pBz15 but with N*-peptide fusion on CbbL, construct used for protein crystallization

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    Form IA Rubisco with CsoS2 N-peptide fusion; CbbL-Npeptide, CbbS
    

    Tags

    CsoS2 N-peptide consensus to CbbL C-terminus and …

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFA-DAB1

    Plasmid

    #162691

    Purpose

    Expresses DabAB1 inorganic carbon transport complex

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    DabAB1 inorganic carbon transport complex
    

    Expression

    Bacterial

    Promoter

    PLtet0-1 promoter

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pYPQ290-D570A-SRDX

    Plasmid

    #129682

    Purpose

    Gateway entry clone for CRISPR-Cas12b systems

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Insert

    AacCas12b-SRDX
    

    Use

    CRISPR; Gateway compatible aaccas12b entry clone

    Expression

    Plant

    Mutation

    D570A; AacCas12b is rice codon optimized

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFE-dabAB2 C539A

    Plasmid

    #133008

    Purpose

    pFE carrying a dabA2 gene (Uniprot: D0KWS7) with a C539A mutation and a dabB2 gene  (Uniprot: D0KWS8)

    Depositor

    David Savage

    Article

    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

    Inserts

    dabB2
    dabA2
    

    Expression

    Bacterial

    Mutation

    C539A

    Promoter

    tetR

    Available Since

    Nov. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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