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Showing: 1551 - 1600 of 15599 results

  2. mCit-APPAPAPPAP-gs

    Plasmid
    #228091
    Purpose
    Mammalian expression of 10 coiled coil segments in APPAPAPPAP arrangement, with short flexible linkers and N-terminal mCitrine for phase separation and forming of liquid condensates in mammalian cells
    Depositor
    Roman Jerala
    Article
    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:
    Insert
    mCit, APPAPAPPAP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    228091-plasmid-map-sequence-id-451163

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  3. MmSphk1

    Plasmid
    #118599
    Purpose
    Expresses sphingosine kinase 1, which catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate in mice
    Depositor
    Kevin Lynch
    Article
    Kharel et al PLoS One. 2018 Apr 19;13(4):e0192179. do
    Insert
    Mus musculus sphingosine kinase 1
    Use
    Tags
    FLAG
    Expression
    Yeast
    Mutation
    Promoter
    GAL1, 10
    Available since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    118599-plasmid-map-sequence-id-234126

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  4. pcDNA3-endo-Nb37

    Plasmid
    #205514
    Purpose
    Endosome-targeted Nb37 nanobody that binds and stabilizes the active form of Gαs G-protein subunit.
    Depositor
    Jin Zhang
    Article
    Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:
    Insert
    endo-Nb37
    Use
    Tags
    2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and mChe…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    205514-plasmid-map-sequence-id-409423

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  5. pMT-ADARcd-E488Q-V5

    Plasmid
    #139686
    Purpose
    Expresses only the hyperactive form of the ADAR catalytic domain - no RNA binding protein. This is a control plasmid to be used with pMT-Hrp48-ADARcd-E488Q-V5
    Depositor
    Michael Rosbash
    Article
    Xu et al RNA. 2018 Feb;24(2):173-182. doi: 10.126
    Insert
    Adenosine deaminase acting on RNA (Adar Fly)
    Use
    Tags
    V5
    Expression
    Bacterial and Insect
    Mutation
    Promoter
    Available since
    March 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    139686-plasmid-map-sequence-id-274450

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  7. mAIRN HO

    Plasmid
    #207411
    Purpose
    Mammalian-cell-based plasmid system that expresses mAIRN (an R-loop-forming transcript) upon dox-induction, which will further cause head-ON transcription-replication conflict (HO TRC).
    Depositor
    Karlene Cimprich
    Article
    Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do
    Insert
    mAIRN
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    March 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    207411-plasmid-map-sequence-id-422696

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  8. mAIRN CD

    Plasmid
    #207412
    Purpose
    Mammalian-cell-based plasmid system that expresses mAIRN (an R-loop-forming transcript) upon dox-induction, which will further cause co-directional transcription-replication conflict (CD TRC).
    Depositor
    Karlene Cimprich
    Article
    Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do
    Insert
    mAIRN
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    March 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    207412-plasmid-map-sequence-id-422689

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  9. mCit-APPAP-gs

    Plasmid
    #228090
    Purpose
    Mammalian expression of five coiled coil segments in APPAP arrangment, with short flexible linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells.
    Depositor
    Roman Jerala
    Article
    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:
    Insert
    mCit, APPAP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    228090-plasmid-map-sequence-id-451134

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  10. ECFP HO

    Plasmid
    #207413
    Purpose
    Mammalian-cell-based plasmid system that expresses ECFP (a non R-loop-forming transcript) upon dox-induction, which will further cause head-ON transcription-replication conflict (HO TRC).
    Depositor
    Karlene Cimprich
    Article
    Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do
    Insert
    ECFP
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Jan. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    207413-plasmid-map-sequence-id-421043

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  11. ECFP CD

    Plasmid
    #207414
    Purpose
    Mammalian-cell-based plasmid system that expresses ECFP (a non R-loop-forming transcript) upon dox-induction, which will further cause co-directional transcription-replication conflict (CD TRC).
    Depositor
    Karlene Cimprich
    Article
    Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do
    Insert
    ECFP
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Jan. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    207414-plasmid-map-sequence-id-421036

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  12. pcDNA3-nuc-Nb37

    Plasmid
    #205515
    Purpose
    Nuclear-targeted Nb37 nanobody that binds and stabilizes the active form of Gαs G-protein subunit.
    Depositor
    Jin Zhang
    Article
    Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:
    Insert
    nuc-Nb37
    Use
    Tags
    Nuclear localization signal (NLS) and mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    205515-plasmid-map-sequence-id-409490

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  13. pTrex-n-eGFP

    Plasmid
    #62544
    Purpose
    Expression of eGFP in T. cruzi. The NheI-linearized form of this plasmid could be efficiently integrated in to T. cruzi genome
    Depositor
    Rick Tarleton
    Article
    Peng et al MBio. 2014 Dec 30;6(1). pii: e02097-14.
    Insert
    eGFP
    Use
    (1)trypanosoma cruzi expression of egfp (2) genom…
    Tags
    None
    Expression
    Mutation
    None
    Promoter
    Ribo-HX1
    Available since
    April 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    62544-plasmid-map-sequence-id-109759

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  17. pDOC-GG

    Plasmid
    #149377
    Purpose
    Chassis plasmid for building mutagenesis cassettes by Golden Gate assembly to form a donor plasmid for recombineering by Gene Doctoring
    Depositor
    Mark Pallen
    Article
    Thomson et al BMC Biotechnol. 2020 Oct 7;20(1):54. doi
    Inserts
    Fragment containing a kanamycin resistance cassette
    Fragment containing pMB1 origin of replication and an I-SceI recognition sequence
    Fragment containing a lacZ⍺ expression cassette flanked by BsaI sites
    Fragment containing an I-SceI recognition sequence
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    149377-plasmid-map-sequence-id-308794

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  21. pYPQ166-iSpyMac

    Plasmid
    #149553
    Purpose
    Gateway entry clone for CRISPR-iSpyMacCas9 genome editing at NAAR PAMs
    Depositor
    Yiping Qi
    Article
    Sretenovic et al Plant Commun. 2020 Jul 22;2(2):100101. d
    Insert
    iSpyMacCas9
    Use
    CRISPR; Gateway compatible zispymaccas9 entry clo…
    Tags
    3X FLAG, NLS and NLS
    Expression
    Plant
    Mutation
    R221K, N394K, Protospacer interacting domain form…
    Promoter
    Available since
    July 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    149553-plasmid-map-sequence-id-306139

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  25. HsCERK

    Plasmid
    #118600
    Purpose
    Expresses ceramide kinase, which catalyzes the phosphorylation of ceramide to form ceramide 1-phosphate
    Depositor
    Kevin Lynch
    Article
    Kharel et al PLoS One. 2018 Apr 19;13(4):e0192179. do
    Insert
    Homo sapiens ceramide kinase
    Use
    Tags
    FLAG
    Expression
    Yeast
    Mutation
    Promoter
    GAL1, 10
    Available since
    Feb. 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    118600-plasmid-map-sequence-id-234128

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  28. pLz74_Rubisco_CsoS2_Npeptide_fusion

    Plasmid
    #140840
    Purpose
    Same as pBz15 but with N*-peptide fusion on CbbL, construct used for protein crystallization
    Depositor
    David Savage
    Article
    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-
    Insert
    Form IA Rubisco with CsoS2 N-peptide fusion; CbbL-Npeptide, CbbS
    Use
    Tags
    CsoS2 N-peptide consensus to CbbL C-terminus and …
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    140840-plasmid-map-sequence-id-296743

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  30. pLV-SFFV-CALU-WPRE-UbC-Emerald

    Plasmid
    #225951
    Purpose
    Lentiviral vector plasmid expressing human calumenin (CALU) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Bart Nieuwenhuis, Evan Reid, Richard Eva
    Inserts
    CALU (CALU Human)
    Emerald
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV and UbC
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    225951-plasmid-map-sequence-id-446853

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  31. pLV-SFFV-CALU-WPRE-UbC-mCherry

    Plasmid
    #225952
    Purpose
    Lentiviral vector plasmid expressing human calumenin (CALU) under the spleen focus-forming virus (SFFV) promoter and mCherry under the Ubiquitin C (UbC) promoter
    Depositor
    Bart Nieuwenhuis, Evan Reid, Richard Eva
    Inserts
    CALU (CALU Human)
    mCherry
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV and UbC
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    225952-plasmid-map-sequence-id-446855

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  32. pLV-SFFV-LNPK-WPRE-UbC-Emerald

    Plasmid
    #225953
    Purpose
    Lentiviral vector plasmid expressing human lunapark (LNPK) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Bart Nieuwenhuis, Evan Reid, Richard Eva
    Inserts
    LNPK (LNPK Human)
    Emerald
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV and UbC
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    225953-plasmid-map-sequence-id-446858

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  33. pLV-SFFV-LNPK-WPRE-UbC-mCherry

    Plasmid
    #225954
    Purpose
    Lentiviral vector plasmid expressing human lunapark (LNPK) under the spleen focus-forming virus (SFFV) promoter and mCherry under the Ubiquitin C (UbC) promoter
    Depositor
    Bart Nieuwenhuis, Evan Reid, Richard Eva
    Inserts
    LNPK (LNPK Human)
    mCherry
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV and UbC
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    225954-plasmid-map-sequence-id-446860

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  34. (P6f)3(P14f)3-gs

    Plasmid
    #228082
    Purpose
    Mammalian expression of coiled coil segments (P6f)x3-(P14f)x3, with short flexible linkers; for phase separation and forming of liquid condensates in mammalian cells.
    Depositor
    Roman Jerala
    Article
    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:
    Insert
    P6f, P14f
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    228082-plasmid-map-sequence-id-454673

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  35. (S1h-S3h)3-gs

    Plasmid
    #228078
    Purpose
    Mammalian expression of coiled coil segments (S1h-S3h)x3, with short flexible linkers; for phase separation and forming of liquid condensates in mammalian cells.
    Depositor
    Roman Jerala
    Article
    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:
    Insert
    S1h, S3h
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    228078-plasmid-map-sequence-id-457750

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  36. TagBFP(S2h)8-gs

    Plasmid
    #228086
    Purpose
    Mammalian expression of coiled coil segments (S2h)x8, with short flexible linkers and N-terminal TagBFP; for phase separation and forming of liquid condensates in mammalian cells.
    Depositor
    Roman Jerala
    Article
    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:
    Insert
    BFP, S2h
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    228086-plasmid-map-sequence-id-454692

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  37. RFP-PPPPPPAAAA-gs

    Plasmid
    #228092
    Purpose
    Mammalian expression of 10 coiled coil segments in PPPPPPAAAA arrangement, with short flexible linkers and N-terminal mCitrine for phase separation and forming of liquid condensates in mammalian cells
    Depositor
    Roman Jerala
    Article
    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:
    Insert
    mCit, PPPPPPAAAA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    228092-plasmid-map-sequence-id-451390

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  38. mCit(S1h)8-gs

    Plasmid
    #228084
    Purpose
    Mammalian expression of coiled coil segments (S1h)x8, with short flexible linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells.
    Depositor
    Roman Jerala
    Article
    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:
    Insert
    mCit, S1h
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    228084-plasmid-map-sequence-id-451170

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  39. TagBFP(S2h)12-gs

    Plasmid
    #228087
    Purpose
    Mammalian expression of coiled coil segments (S2h)x12, with short flexible linkers and N-terminal TagBFP; for phase separation and forming of liquid condensates in mammalian cells.
    Depositor
    Roman Jerala
    Article
    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:
    Insert
    BFP, S2h
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    228087-plasmid-map-sequence-id-451132

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  40. pLVUT-spC3GFP

    Plasmid
    #225487
    Purpose
    Tet-on lentiviral vector for controlled expression of a secretable and permeable form of C. botulinum exoenzyme C3 transferase fused with EGFP
    Depositor
    Robert Gross
    Article
    Gutekunst et al Neuroscience. 2016 Dec 17;339:308-318. d
    Insert
    Secretable and permeable exoenzyme C3 transferase
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Amino acids 30-251
    Promoter
    Human ubiquitin C
    Available since
    Sept. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    225487-plasmid-map-sequence-id-444518

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  41. pVioCD53

    Plasmid
    #191130
    Purpose
    Expression of vioC, truncated vioD and tdMCP form a J23105 constitutive promoter
    Depositor
    Ariel Lindner
    Article
    Guo et al Cell. 2022 Sep 29;185(20):3823-3837.e23.
    Inserts
    vioC (vioC Synthetic)
    vioD truncated
    dMCP off-frame
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    C-terminal truncation from 264th amino acid
    Promoter
    J23105 constitutive promoter
    Available since
    June 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    191130-plasmid-map-sequence-id-382518

    Enlarge

  46. pV5endoNA2

    Plasmid
    #174729
    Purpose
    Expresses V5-endoNA2 fusion protein for detection of polysialic acid. The endoNA2 is a catalytically inactive form of endosialidase from bacteriophage PK1A2.
    Depositor
    Jukka Finne
    Article
    Lehti et al Mol Cancer Ther. 2021 Jul 26. pii: 1535-
    Insert
    V5 epitope tag
    Use
    Tags
    Histag
    Expression
    Bacterial
    Mutation
    Promoter
    T5
    Available since
    Sept. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    174729-plasmid-map-sequence-id-343520

    Enlarge

  48. pLV-SFFV-CKAP4-WPRE-UbC-Emerald

    Plasmid
    #225943
    Purpose
    Lentiviral vector plasmid expressing human cytoskeleton associated protein 4 (CKAP4) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Bart Nieuwenhuis, Evan Reid, Richard Eva
    Inserts
    CKAP4 (CKAP4 Human)
    Emerald
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV and UbC
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    225943-plasmid-map-sequence-id-446819

    Enlarge

Showing: 1551 - 1600 of 15599 results