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Plasmid#196526PurposeEntry vector for Gateway with Eps8L2DepositorInsertEps8L2-FL (EPS8L2 Human)
UseTagsExpressionBacterialMutationPromoterCMV promoter; T7 promoterAvailable sinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC6A6
Plasmid#132196PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC6A6 (SLC6A6 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 28, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC19-hAPOE-R-loop
Plasmid#134901PurposeGeneration and purification of R-loops for in vitro biochemistryDepositorInsertAPOE promoter (APOE Human)
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
HTR6-DuET
Plasmid#213326PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertHTR6 (HTR6 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC6A19
Plasmid#132231PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC6A19 (SLC6A19 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 8, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR221_SLC9C1
Plasmid#132247PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC9C1 (SLC9A10 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
HTR4-DuET
Plasmid#213324PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertHTR4 (HTR4 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC9A2
Plasmid#132222PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC9A2 (SLC9A2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 7, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR221_SLC5A2
Plasmid#132241PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC5A2 (SLC5A2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR221_SLC2A3
Plasmid#132114PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC2A3 (SLC2A3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 4, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pENT-L1-Kozak-Ensconsin-3XGFP-stop-L2
Plasmid#186361PurposeEntry clone with ORF encoding the MT-binding domain of Ensconsin fused to 3 copies of GFP in C-terminal, flanked by Gateway recombination sequences.DepositorInsertHuman Ensconsin (MAP7 Human, Synthetic)
UseExpression of a fluorescent microtubule markerTags3xEGFPExpressionMutationPromoterAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC25A45
Plasmid#132054PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC25A45 (SLC25A45 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 4, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
p2R3a-Tq2CFP-OcsT
Plasmid#71268PurposeEntry clone containing Turqoise2. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway systemDepositorInsertTq2CFP
UseGatewayTags4xGly linker and octaline synthase terminatorExpressionMutationPromoterAvailable sinceDec. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pENTR-myr-AKT-HA
Plasmid#31790DepositorInsertAkt1 (Akt1 Mouse)
UseEntry vectorTagsHA and MyrExpressionMutationPromoterAvailable sinceOct. 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-B-GRAM-W-NeoR
Plasmid#211710PurposeLentiviral expression of a dimerization-dependent fluorescent protein (ddFP) subunit (B) fused with the GRAM domain of GRAMD1b carrying a G187W mutation (B-GRAM-W)DepositorInsertB-tagged GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
UseLentiviralTagsddFP-B3ExpressionMammalianMutationChanged Glycine 187 to TryptophanPromoterCMVAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Kv2.1C-linker-TlcnC
Plasmid#114377Purposeexpresses Flpo recombinase-dependent GtACR2-Kv2.1C-linker-TlcnC, which targets GtACR2 to the somatodendritic compartment. Messier et al found this hybrid construct was the most effective at targetingDepositorInsertGtACR2-EYFP-Kv2.1C-linker-TlcnC (NEWENTRY )
UseAAVTagsEYFP and Kv2.1C-linker-TlcnCExpressionMammalianMutationPromoterEf1aAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SV2C
Plasmid#132297PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSV2C (SV2C Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR221_SLC22A8
Plasmid#131989PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC22A8 (SLC22A8 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 22, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
8xNFAT-ZsG-muhCD4
Plasmid#162745PurposeNFAT-driven ZsGreen-1 reporter gene with mutated human CD4 geneDepositorInsert8x of NFAT binding motif follwed by ZsGreen-1 fluorescent protein gene (CD4 Human)
UseRetroviralTagsExpressionMammalianMutationHuman CD4 gene with glutamine to tyrosine at posi…PromoterAvailable sinceApril 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC35F2
Plasmid#132121PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC35F2 (SLC35F2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 25, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits