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pRK5 CASTOR2-Flag Cterm
Plasmid#84490Purposeoverexpression of CASTOR2 with Flag tagDepositorInsertCASTOR2 (GATSL2 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceOct. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
gRNA_DNMT3a-T2
Plasmid#41822PurposeExpresses a guide RNA (gRNA) to target DNMT3a (T2 target sequence) for genome engineeringDepositorInsertgRNA_DNMT3a-T2 (DNMT3A Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
shPBRM1 #122-Tet-pLKO-puro
Plasmid#107401PurposeLentiviral expression of shRNA targeting PBRMDepositorInsertshRNA targeting PBRM1 (PBRM1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBabe puro RalA S31N
Plasmid#19718DepositorInsertV-ral simian leukemia viral oncogene homolog A (RALA Human)
UseRetroviralTagsExpressionMammalianMutationS31N mutant for dominant neg function. NOTE: lo…PromoterAvailable sinceOct. 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-jGCaMP8f F366H
Plasmid#204141PurposeFluorescent reporter for calcium imaging in mammalian cells - jGCaMP8f with F366H mutationDepositorInsertjGCaMP8f F366H
UseTagsN-terminal His tagExpressionMammalianMutationPhenylalanine 366 changed to histidinePromoterCMVAvailable sinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti_Puro_DEST mouse_p65promoter_mouse_p65-Clover
Plasmid#124549PurposeLentiviral vector to express p65-Clover under p65 promoter (With puromycin resistance)DepositorInsertp65 promoter- p65 CDS-Clover
UseLentiviralTagsCloverExpressionMammalianMutationPromoterAvailable sinceMay 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRQ-hRasV12
Plasmid#18113DepositorInserth-RasV12G (HRAS Human)
UseRetroviralTagsExpressionMammalianMutationG12V, constitutively activePromoterAvailable sinceFeb. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
EGFP-uTEV1-CB5
Plasmid#171007PurposeHiLITR protease with CB5 C-terminal tmd targeting information (ER)DepositorInsertEGFP-uTEV1-CB5(tmd)
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterpTRE-tight (TetOn)Available sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHIS3p:yomRuby3-Tub1+3'UTR::LEU2
Plasmid#204288Purposeyeast codon optimized mRuby3 alpha-tubulin plasmid for integration into the endogenous TUB1 ORFDepositorInsertpHIS3p:yomRuby3-Tub1+3'UTR::LEU2
UseTagsmRuby3ExpressionYeastMutationPromoterpHis3Available sinceJuly 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
Axin1_HA in pRK5
Plasmid#154220PurposeC-terminal HA-tagged AXIN1 cDNA expression vector for transfectionsDepositorInsertAXIN1 (AXIN1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceNov. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-OSF-BECN1
Plasmid#99328PurposeComponent for mammalian expression of recombinant PI3KC3-C1 complexDepositorInsertBeclin 1 (BECN1 Human)
UseTagsStrep-Strep-FlagExpressionMammalianMutationPromoterCMVAvailable sinceAug. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
CMV-hCas9-T2A-miRFP670
Plasmid#187223PurposeExpresses human Cas9 nuclease and miRFP670 via T2A linker under control of a CMV promoter.DepositorInsertCas9
UseTagsInserted T2A-miRFP670 at 3' terminal of MCS.ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMM627
Plasmid#112532PurposepZE2-PLhrtO-lux-hrtR-RBS2-chuA - Composite plasmid of pMM534 and pMM549, ColE1 origin, KanRDepositorInsertsHrtR
ChuA
luxCDABE
UseTagsExpressionBacterialMutationPromoterJ23107, PL(HrtO), and ProDAvailable sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSMP-SUZ12_1
Plasmid#36390DepositorInsertSUZ12 (SUZ12 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSMP-EZH1_2
Plasmid#36360DepositorInsertEZH1 (EZH1 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDest27-mBIN1
Plasmid#61086PurposeExpress N-terminal GST tagged-mouse BIN1 in mammalian cellsDepositorInsertsmallest constitutive BIN1excluding exon 7, 11, 13-17
UseTagsGSTExpressionMammalianMutationPromoterCMVAvailable sinceJan. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
gRNA_DNMT3b
Plasmid#41823PurposeExpresses a guide RNA (gRNA) to target DNMT3b for genome engineeringDepositorInsertgRNA_DNMT3b (DNMT3B Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dRanCas13b-EGFP-NLS-3xFlag
Plasmid#132409Purposeoverexpression in human cellsDepositorInsertdRanCas13b
UseLentiviralTagsflagExpressionMammalianMutationH147A; H1044APromoterAvailable sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSP64-hKCNE1
Plasmid#53049PurposeExpresses beta subunit for IKs channel in Xenopus oocytesDepositorInsertPotassium voltage-gated channel subfamily E member 1 (KCNE1 Human)
UseXenopus oocyte expressionTagsExpressionMutationPromoterSP6Available sinceMay 7, 2014AvailabilityAcademic Institutions and Nonprofits only
