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Plasmid#70147PurposeExpresses a DHFR-mCherry fusion protein and uses for a CRISPR gene deletionDepositorInserta selectable dihydrofolate reductase-thymidylate synthase marker
UseExpression in toxoplasma gondiiTagsmCherryExpressionMutationPromoterDHFR 5' UTRAvailable sinceNov. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-Solo-mScarlet-H2BC11
Plasmid#207756PurposeDonor template for mScarlet insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755DepositorArticleInsertH2BC11 Homology Arms flanking a mScarlet Tag (H2BC11 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
FLAG SMG1 T3550A/S3556A
Plasmid#199574PurposeExpresses FLAG-tagged mutated SMG1DepositorInsertSMG1 (SMG1 Human)
UseTagsFLAGExpressionMammalianMutationT3550A and S3556APromoterCMVAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO V5 HSPA2
Plasmid#19512DepositorInsertheat shock 70kDa protein 2 (HSPA2 Human)
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
UKNP6.1.1
Plasmid#98386PurposeExpression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)DepositorInsertHCV E1E2
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-nuc-Nb80
Plasmid#205519PurposeNuclear-targeted Nb80 nanobody that binds and stabilizes β2AR active conformation, blocking Gαs coupling.DepositorInsertnuc-Nb80
UseTagsNuclear localization signal (NLS) and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyn-dPlcR
Plasmid#181832PurposeGenetically encoded single-color sensor for monitoring plasma membrane PI(4,5)P2 dynamics in living cells. Based on dimerization-dependent green fluorescent protein (ddGFP).DepositorInsertLyn-PlcR
UseTagsN-terminal targeting sequence from Lyn kinase, dd…ExpressionMammalianMutationPromoterCMVAvailable sinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2/EV
Plasmid#120833PurposeControl vector, 20nt random sequence between gateway recombination sitesDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_LacZ
Plasmid#25893DepositorInsertLacZ
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 3xFlag IFIT5
Plasmid#53556Purposemammalian expression of IFIT5DepositorInsertIFIT5 (IFIT5 Human)
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMay 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag Jnk1a1
Plasmid#13798DepositorInsertJnk1a1 (MAPK8 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ExRai-AktAR
Plasmid#118462PurposecpGFP-based excitation-ratiometric biosensor for monitoring Akt/Protein Kinase B activity.DepositorInsertExRai-AktAR
UseTags6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNAHygro(+)LAMP2AS
Plasmid#86146PurposeAnti sense for LAMP2DepositorInsertLAMP2a (LAMP2 Human)
UseTagsExpressionMammalianMutationcontains first 525 bases of LAMP cDNAPromoterCMVAvailable sinceFeb. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ICUE3
Plasmid#61622PurposeFRET-based biosensor for monitoring cAMPDepositorInsertICUE3 (RAPGEF3 Human)
UseTagsECFP and cpVenus[L194]ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
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MELOPS
Plasmid#80151PurposeGenetically encoded melanosome-localized pH sensor.DepositorInsertOCA2 (OCA2 Human)
UseTagsmNectarine in first luminal loopExpressionMammalianMutationnonpathologic polymorphism D257A, pore mutation V…PromoterCMVAvailable sinceSept. 20, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCDNA3 Flag p38 gamma
Plasmid#20353DepositorInsertpDCNA3 Flag p38 gamma (Mapk12 Rat)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceApril 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
hGATA-1 WT
Plasmid#118352PurposeThis plasmid expresses human GATA-1 WT.DepositorInserthGATA-1 (GATA1 Human)
UseTagsmyc and 6xHISExpressionMammalianMutationWTPromoterAvailable sinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
syntaxin3-myc-myc-His
Plasmid#12372DepositorInsertSyntaxin3 (STX3 Human)
UseTags2xMyc-HisExpressionMammalianMutationInsert is cloned into a modified vectorPromoterAvailable sinceSept. 28, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-TK1
Plasmid#100544Purposemammalian expression of thymidine kinase 1DepositorInsertthymidine kinase 1 (TK1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only