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Plasmid#120488PurposeBarcoded lentiviral vector to express KLF1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertKLF1 (KLF1 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSL1180-HR-PUbEBFP2-NLS
Plasmid#176682PurposeExpression of nuclear localized EBFP2 under Ae. aegypti Polyubiquitin promoter (AAEL003888), EBFP2 version of pSL1180-HR-PUbECFP (Addgene #47917)DepositorInsertEBFP2
UseTagsExpressionInsectMutationPromoterAe. aegypti poly-ubiquitin (AAEL003888)Available sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Puro-sgNT
Plasmid#138681PurposeExpresses a non-targeting sgRNA and Cas9DepositorInsertsgNT
UseLentiviralTagsExpressionMutationPromoterhU6Available sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCSD_2XNLS-SpCas9WT-NLS-3XHA-2XNLS-NmdCas9-NLS
Plasmid#107327PurposeExpresses SpCas9 fused to dNmCas9 in mammalian cellsDepositorInsertSpCas9-NmCas9 fusion
UseCRISPRTagsNLSExpressionMammalianMutationSpCas9 is fused to nuclease dead NmCas9 (D16A, D5…PromoterCMV IE94Available sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
psigma2-EGFP
Plasmid#53610PurposeExpresses sigma2 of adaptor protein of AP2DepositorInsertsigma2 (Ap2s1 Rat)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-flex-lck-smV5-4x6T
Plasmid#196419PurposeCre-dependent AAV expression of membrane-targeted V5 spaghetti monster reporter preferentially in astrocytes; astrocyte selectivity generated with 4x6T miRNA targeting cassetteDepositorInsertLck-smV5
UseAAV and Cre/Lox; Astrocyte-selectiveTagsLckExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-Nsp7
Plasmid#157688Purposemammalian expression of untagged SARS-CoV-2 Nsp7 under control of a tetracycline-inducible promoterDepositorInsertNsp7 (ORF1ab SARS-CoV-2)
UseTagsExpressionMammalianMutationcodon optimizedPromoterAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
LentiV_Neo_Sik3
Plasmid#119972PurposeLentiviral expression plasmid of mouse Sik3 cDNA with neomycin resistance geneDepositorInsertSik3 (Sik3 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterEFS promoterAvailable sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEN765 -pCAGGS-3XFLAG-mKate2-(human)PDS5B-bpA
Plasmid#156436PurposeFor transient expression of human PDS5B tagged with mKate2DepositorInserthuman PDS5B (PDS5B Human)
UseTagsExpressionMammalianMutationnonePromoterAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKS035 - pCAGGS-3XFLAG-Rad21-mKate2-bpA
Plasmid#156445PurposeFor transient expression of mouse RAD21 (SCC1) tagged with mKate2DepositorInsertmouse RAD21 (Rad21 Mouse)
UseTagsExpressionMammalianMutationnonePromoterAvailable sinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pmRFP-N1 human cofilin WT
Plasmid#50856Purposeexpresses human cofilin fused to mRFP in mammalian cellsDepositorInsertcofilin 1 (CFL1 Human)
UseTagsmRFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pKS021 - pCAGGS-3XFLAG-mKate2-SA1-bpA
Plasmid#156440PurposeFor transient expression of mouse SA1 (= STAG1 or SCC3A) tagged with mKate2DepositorInsertmouse SA1 (Stag1 Mouse)
UseTagsExpressionMammalianMutationnonePromoterAvailable sinceSept. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
EF1a_ETV2_P2A_Hygro_Barcode
Plasmid#120436PurposeBarcoded lentiviral vector to express ETV2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertETV2 (ETV2 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceApril 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
p2Flag CMV2-ZO-2
Plasmid#27415DepositorInsertZonula Occludens 2 (TJP2 Human)
UseTags2xFlagExpressionMammalianMutationPromoterAvailable sinceMarch 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
pKS071 - pCAGGS-3XFLAG-mKate2-(human)PDS5A-bpA
Plasmid#156449PurposeFor transient expression of human PDS5A tagged with mKate2DepositorInserthuman PDS5A (PDS5A Human)
UseTagsExpressionMammalianMutationnonePromoterAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1_WT RAD23B
Plasmid#201457PurposeExpresses human RAD23B with an N-terminal GST tag in E. coliDepositorInsertUV excision repair protein RAD23 homolog B (RAD23B Human)
UseTagsGSTExpressionBacterialMutationCoding sequence has been optimised for expression…Promotertac promoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1_WT RAD23A
Plasmid#201456PurposeExpresses human RAD23A with an N-terminal GST tag in E. coliDepositorInsertUV excision repair protein RAD23 homolog A (RAD23A Human)
UseTagsGSTExpressionBacterialMutationCoding sequence has been optimised for expression…Promotertac promoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABE6.3
Plasmid#102916Purposeexpresses ABE6.3 in mammalian cellsDepositorInsertABE6.3
UseTagsNLSExpressionMammalianMutationsee manuscriptPromoterCMVAvailable sinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
TetO-FUW-DN-REST
Plasmid#61533PurposeExpresses DN-REST in mammalian cellsDepositorInsertREST (REST Human)
UseLentiviralTagsExpressionMammalianMutationP73, Dominant negative REST (contains the Zf DNA-…PromoterAvailable sinceJan. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-G2-ATP8-V5 (puromycin)
Plasmid#86852Purposemammalian expression of ATP8 with G2 mitochondrial targeting sequence and V5 tagDepositorInsertATP8 (ATP8 Human)
UseTagsATP5G2 (G2) mitochondrial targeting signal and V5ExpressionMammalianMutationcodon optimizedPromoterCMVAvailable sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only