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Plasmid#107455PurposeeMscL G22S is optimized for mammalian rodent neuronal expression to the plasma membrane through a neuron-specific promoter and a voltage-gated channel targeting motifDepositorInsertbacterial mechanosensitive ion channel of large conductance (mscL Escherichia coli)
UseAdeno-associated viralTagsKir2.1 ER export signal (FCYENEV) and tdTomato fl…ExpressionMammalianMutationBearing a glycine to serine substitution at posit…Promoterhuman synapsin 1Available sinceApril 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-jGCaMP8f-WPRE
Plasmid#162376PurposeAAV-mediated expression of ultrafast protein calcium sensor under the Syn promoterDepositorHas ServiceAAV Retrograde, AAV1, and AAV9InsertjGCaMP8f
UseAAVTags6xHisExpressionMammalianMutationQ315LPromoterSynapsinAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 TaGu N-1F
Plasmid#203762PurposeFor expression of N-flag tagged TaGu R2 protein in mammalian cellsDepositorInsertR2 retroelement protein
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMS2M-PH-gagpol-D64V
Plasmid#166031PurposeExpression of bacteriophage-derived MS2 coat protein fused HIV-1 Lentiviral Gag-Pol at N terminal with a HIV-1 protease cleavage signal between MS2 and Gag-Pol.DepositorInsertMS2-Gag-Pol
UseLentiviralTagsExpressionMutationD64V mutation within the integrase within PolPromoterAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEE515
Plasmid#149288PurposeT-DNA encoding TRV2 complete genome with MCSDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
p426-SNR52p-gRNA.CAN1.Y-SUP4t
Plasmid#43803PurposeEncodes a gRNA that targets Can1.Y in yeast.DepositorArticleInsertCAN1.y gRNA (CAN1 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterSNR52Available sinceMarch 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pPGK-T7/2-CD44s (fl)
Plasmid#137812Purposeexpression of CD44 proteins in mammalian cellsDepositorInsertCD44s (fl) (CD44 Human)
UseTagsGFPExpressionMammalianMutationfull length, I186T and I253V on CD44PromoterPGKAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLifeAct_mScarlet-i_N1
Plasmid#85056PurposeIn vivo visualization of actin cytoskeleton (can be used for colocalization studies)DepositorInsertLifeAct
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-PM
Plasmid#197386PurposeTo constitutively express Pdx1-Mafa and in cells (Blasticidin resistant).DepositorInsertPDX1-T2A-MAFA
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.GCaMP6f.WPRE.SV40
Plasmid#100836PurposeAAV expression of ultrasensitive protein calcium sensor from CAG promoterDepositorHas ServiceAAV1, AAV2, and AAV9InsertGCaMP6f
UseAAVTagsExpressionMammalianMutationGCaMP3-T302L R303P A317E D380Y T381R S383T R392GPromoterCAGAvailable sinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1 METTL3
Plasmid#200731PurposeBacterial expression of GST-Tagged codon optimized human METTL3DepositorInsertMETTL3 (METTL3 Human)
UseTagsGST from backbone plasmidExpressionBacterialMutationCodon optimized for expression in E. coliPromoterAvailable sinceJuly 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
LifeAct-14 (LA-14)
Plasmid#158750PurposeLentiviral plasmid for expression of shorter LifeAct variant (up till 14 amino acids) fused with carboxy terminus eGFP in mammalian cells.DepositorInsertLifeAct-14
UseLentiviralTagseGFPExpressionMammalianMutationDeletion mutation for last three amino acids from…PromoterCMV immediate earlyAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-iCAP-AAV9.452sub.LUNG1
Plasmid#184592Purposenon-standard AAV2 rep-AAV9.452sub.LUNG1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system for mouse lung transduction after intravenous injectionDepositorInsertSynthetic construct isolate AAV9.452sub.LUNG1 VP1 gene
UseAAVTagsExpressionMammalianMutation7 amino acid substitution between VP1 452 and VP1…Promoterp41Available sinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDragon-p21
Plasmid#155016PurposeFor Flp-mediated cassette exchange. Expresses tdTomato in the presence of (r)tTA, switching to the cell cycle suppressor p21 in the presence of Cre and (r)tTA activityDepositorInsertCdkn1a (Cdkn1a Mouse)
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
Flag-JunWT-Myc
Plasmid#47443Purposemammalian expression of WT c-JunDepositorInsertJun (Jun Mouse)
UseTagsFlag and mycExpressionMammalianMutationPromoterCMVAvailable sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
SpyTag003-mKate2
Plasmid#133452PurposeExpresses SpyTag003-mKate2 (a far-red fluorescent protein) in bacterial cytoplasmDepositorInsertSpyTag003-mKate2
UseTagsGSGSGS linker and His6ExpressionBacterialMutationPromoterT7Available sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEV-G4-Km
Plasmid#46819PurposeTEF1 and HXT7 promoter controlled expression cassettes with G418 resistance for yeast transformationDepositorTypeEmpty backboneUseTagsFLAG and c-mycExpressionYeastMutationPromoterTEF1 and HXT7Available sinceSept. 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-RAC1
Plasmid#116783PurposeLentiviral expression of RAC1DepositorInsertRAC1 (RAC1 Human)
UseLentiviralTagsExpressionMutationWTPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-25:MAP1LC3B-mEGFP
Plasmid#101783PurposeHomology arms and mEGFP-linker sequence for N-terminus tagging of human MAP1LC3BDepositorInsertMAP1LC3B Homology Arms with mEGFP-linker (MAP1LC3B Human)
UseCRISPR; Donor templateTagsmEGFP-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceOct. 31, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNCST-avGFP
Plasmid#129508PurposeExpresses avGFP (Lambert 2019 clone) constitutively in E. coli (most strains)DepositorInsertavGFP
UseTagsExpressionBacterialMutationPromotersynthetic constitutive (stationary phase) promote…Available sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.2_mTagBFP2
Plasmid#113725PurposeExpresses the fluorescent protein mTagBFP2 in a lentiviral backboneDepositorInsertmTagBFP2
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMIH-TOMM20-Halo
Plasmid#111626PurposeFusion protein used to visualise mitochondria upon the addition of Halo specific dye, has hygromycin selectionDepositorInsertTOMM20 (Tomm20 Mouse)
UseRetroviralTagsHaloTagExpressionMammalianMutationPromoterMSCVAvailable sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET His6 MBP TEV LIC cloning vector (2M-T)
Plasmid#29708DepositorTypeEmpty backboneUseTagsHis6-MBP-TEVExpressionBacterialMutationPromoterAvailable sinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
IDG_TMC3_OE_1
Plasmid#161629PurposeOverexpresses TMC3DepositorInsertTMC3 (TMC3 Human)
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET15b_Bst-LF
Plasmid#153313PurposeBacterial expression of the Bst Large FragmentDepositorInsertBst Large Fragment
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pRSET-QUE2m
Plasmid#129350PurposeATP measurementDepositorInsertQUEEN-2m
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
hSyn1-sypHy-RGECO
Plasmid#84078PurposeCombined fluorescent reporter for both presynaptic calcium influx and vesicle excoytosisDepositorInsertsypHy-RGECO
UseTagsExpressionMammalianMutationPromoterhSyn1Available sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+ hnRNPK myc HIS
Plasmid#71662PurposeMammalian expression of hnRNPKDepositorInserthnRNPK (HNRNPK Human)
UseTagsHIS and mycExpressionMammalianMutationPromoterCMVAvailable sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG:ECFP
Plasmid#32597DepositorInsertECFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 20, 2014AvailabilityAcademic Institutions and Nonprofits only