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Plasmid#124812PurposeHighly resposive GFP-based pyruvate nanosensor.DepositorInsertPyronicSF
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-tetON-dxCas9(3.7)-VPR-EF1a-TagRFP-2A-tet3G
Plasmid#167937PurposeInducible CRISPRa expressionDepositorInsertdxCas9(3.7)-VPR
UseLentiviralTagsExpressionMutationPromotertetONAvailable sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 236HSC WT (BRCA2)
Plasmid#16246DepositorInsertBRCA2 (BRCA2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2007AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-XPO1
Plasmid#116804PurposeLentiviral expression of XPO1DepositorInsertXPO1 (XPO1 Human)
UseLentiviralTagsExpressionMutationWTPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRK5-p18-FLAG
Plasmid#42331DepositorInsertp18 (LAMTOR1 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMV, SP6Available sinceMarch 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
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ND4-DdCBE- left side TALE
Plasmid#157844Purposeexpresses left-side TALE–DddAtox half ND4-DdCBE in mammalian cellsDepositorInsertSOD2 MTS–3x-HA–ND4 left tale–G1397 DddA-C–UGI–SOD2 3'UTR
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYTK001
Plasmid#65108PurposePart Plasmid Entry Vector to be used in the Dueber YTK system.DepositorInsertnone
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
Flag-GFP-TurboID-NES_pLX304
Plasmid#202019Purposeexpresses GFP-TurboID in the mammalian cytosol, lentiviral vectorDepositorInsertFlag-GFP-TurboID-NES
UseLentiviralTagsFlag and GFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 CMV 3xFG eIF4G1
Plasmid#158775PurposepcDNA5 transfection plasmid for the exogenous expression of N-terminal 3xFlag-tagged eIF4G1 isoform lacking the microexon.DepositorInserteIF4G1
UseTags3xFlagExpressionMammalianMutationPromoterAvailable sinceAug. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
4xmts-mNeonGreen
Plasmid#98876PurposeIn vivo visualization of mitochondria (can be used for colocalization studies)DepositorArticleInsert4xmts
UseTagsmNeonGreenExpressionMammalianMutationPromoterCMVAvailable sinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
Luc-T20
Plasmid#177941PurposeTransient mammalian expression of Luc2 (firefly luciferase) along with SARS-CoV-2 sequence 20080-22222 (T20) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.DepositorInsertLuc2 (ORF1ab )
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
3XFlag DVL2 (WT)
Plasmid#24802DepositorInsertDVL2 (DVL2 Human)
UseTags3XFlagExpressionMammalianMutationPromoterAvailable sinceMay 14, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
cytoplasmic NAD+ biosensor
Plasmid#186787PurposeNAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag to target the cytoplasmDepositorInsertcytoplasmic NAD+ biosensor
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
m6A-Tracer-NES
Plasmid#159607PurposeConstitutive transient mammalian expression of m6A-Tracer protein (Kind et al. 2013) with a C-terminal HIV-1 Rev Nuclear Export Signal to reduce background due to nonspecific DNA interactions.DepositorInsertm6A-Tracer-NES
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
mCherry-CEPT1
Plasmid#119079PurposeVisualize localization of ER enzyme CEPT1, which converts diacylglycerol precursors to phosphatidylethanolamine or phosphatidylcholineDepositorInsertCEPT1 (CEPT1 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDCC6
Plasmid#59985PurposeBi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9 and U6>sgRNA cassetteDepositorInsertsCas9
U6-2>sgRNA
UseCRISPRTags3xFLAGExpressionMutationPromoterU6-96Ab and hsp70BbAvailable sinceOct. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Puro-dTAG
Plasmid#207694PurposeEmpty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-dTAG cassetteDepositorTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
TDP-REGv2(mScarlet-FLAG)#9 in pTwist-CMV backbone
Plasmid#216153PurposeExpresses mScarlet in cells with TDP-43 loss-of-function. Has one of the best dynamic ranges of those tested (~100-fold) in SK-N-BE2 cells (brighter than #7). It uses a single intron. [Code 'B11']. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor CommentsDepositorInsertmScarlet with cryptic splice site and C-terminal FLAG tag
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4_10xUAS-MLPmin-luc2
Plasmid#194383PurposeReporter plasmid for split TEV assays; firefly luciferase reporter gene driven by 10x clustered UAS binding sites linked to a minimal MLP promoterDepositorInsert10xUAS-luc2
UseTagsExpressionMammalianMutationPromoterMLP minimalAvailable sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only