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PurposePart Plasmid Entry Vector to be used in the Dueber YTK system.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 65108 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepYTK001
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH10B
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namenone
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SpeciesSynthetic
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BsmBI (destroyed during cloning)
- 3′ cloning site BsmBI (destroyed during cloning)
Resource Information
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Addgene Notes
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This entry vector is used for the construction of any part type. For more information, please visit the supplemental information in PMID:25871405.
This plasmid has been found to be prone to multimerization. Multimerization often does not impact plasmid function, but may reduce transformation efficiencies. You may need to screen multiple colonies to isolate the monomeric version of this plasmid. If you still have trouble isolating the monomeric version, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pYTK001 was a gift from John Dueber (Addgene plasmid # 65108 ; http://n2t.net/addgene:65108 ; RRID:Addgene_65108) -
For your References section:
A Highly-characterized Yeast Toolkit for Modular, Multi-part Assembly. Lee ME, DeLoache WC, Cervantes B, Dueber JE. ACS Synth Biol. 2015 Apr 14. 10.1021/sb500366v PubMed 25871405