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pCMV-2E2-HA-mEGFP
Plasmid#129595PurposeThe encoded protein is the anti-HA frankenbody variant fused with the mEGFP. It can be used to track mature and nascent HA tagged proteins in living organism.DepositorInsertAnti-HA frankenbody variant-mEGFP (2E2-HA scFv-mEGFP)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SNAP-CRY2-VHH(GFP)
Plasmid#58370PurposeFor use in light-induced inactivation of GFP fused protein through the interaction with CIB1-MPDepositorInsertCRY2[1-498] (CRY2 Mustard Weed)
UseTagsSNAP and vhhGFP4ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPHR-iRFP713
Plasmid#103818PurposeSoluble BLInCR mock effector that is recruited to 'localizer' sites upon blue light illuminationDepositorInsertPHR-iRFP713 (CRY2 Mustard Weed)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
dSpyCas9-p300-P2A-CASANOVA
Plasmid#113129PurposeCo-expresses dSpyCas9 fused to the p300 histone acetyltransferase core domain and CASANOVADepositorInsertdCas9-p300-P2A-CASANOVA
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
iRFP713-N2-Frankenbody-Flag
Plasmid#175291PurposeVisualize endogeneous loci coupled with ZF probes harboring 10xFLAG tagDepositorInsertanti-FLAG frankenbody fused with iRFP713
UseTagsiRFP713ExpressionMammalianMutationPromoterAvailable sinceDec. 3, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pT2-cryR;dll4P1Egfp
Plasmid#90160Purposecontains dll4 specific gene promoter driving expression of eGFPDepositorInsertdll4P1Egfp (dll4 Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
Bin1 delta N-BAR
Plasmid#168282PurposeExpress N-BAR-deleted Bin1 mutant (aa 268-476)DepositorInsertBin1 (Bin1 Mouse)
UseTagsExpressionMammalianMutationaa 268-476PromoterAvailable sinceMay 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;lmo2P1Egfp
Plasmid#90162Purposecontains lmo2 specific gene promoter driving expression of eGFPDepositorInsertlmo2P1Egfp (lmo2 Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPHR-EYFP-NLS
Plasmid#103820PurposeSoluble BLInCR mock effector that is recruited to 'localizer' sites upon blue light illuminationDepositorInsertPHR-EYFP-NLS (CRY2 Mustard Weed)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
PHR-GFP
Plasmid#183926PurposeOptogenetic PHR domain coupled to GFP; binds to CIBN upon blue light exposureDepositorInsertPHR-GFP (CRY2 Mustard Weed, Synthetic)
UseTagsExpressionMammalianMutationnonePromoterCMVAvailable sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
AcrIIC1X
Plasmid#128112PurposeAcrX expression in mammalian cells; AcrX is an engineered anti-CRISPR protein targeting S. aureus Cas9DepositorInsertAcrX (AcrIIC1 N3F/D15Q/A48I)
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPHR-EYFP
Plasmid#103817PurposeSoluble BLInCR mock effector that is recruited to 'localizer' sites upon blue light illuminationDepositorInsertPHR-EYFP (CRY2 Mustard Weed)
UseTagsEYFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;dusp5P1Egfp
Plasmid#90161Purposecontains dusp5 specific gene promoter driving expression of eGFPDepositorInsertdusp5P1Egfp (dusp5 Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Akt2(K14A/R25E)-Halo-PS
Plasmid#86632PurposeExpresses eGFP-Akt2-HaloTag-PS fusion (cytosolic tandem)DepositorInsertAkt2(K14A/R25E)-Halo-PS (AKT2 )
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Frankenbody-FLAG-mEGFP
Plasmid#175293PurposeTrack mature and nascent FLAG tagged proteins in live cellsDepositorInsertanti-FLAG frankenbody fused with mEGFP
UseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Frankenbody-FLAG-Halo
Plasmid#175296PurposeTrack mature and nascent FLAG tagged proteins in live cellsDepositorInsertanti-FLAG frankenbody fused with HaloTag
UseTagsHaloTagExpressionMammalianMutationPromoterAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Frankenbody-FLAG-mRuby2
Plasmid#175294PurposeTrack mature and nascent FLAG tagged proteins in live cellsDepositorInsertanti-FLAG frankenbody fused with mRuby2
UseTagsmRuby2ExpressionMammalianMutationPromoterAvailable sinceOct. 7, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCMV-Frankenbody-FLAG-SNAP
Plasmid#175295PurposeTrack mature FLAG tagged proteins in live cellsDepositorInsertanti-FLAG frankenbody fused with SNAP-tag
UseTagsSNAP-tagExpressionMammalianMutationPromoterAvailable sinceOct. 19, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
CD8-GFP-FKBP
Plasmid#100751PurposeExpression of GFP-FKBP at the plasma membrane in mammalian cells. Plasma membrane targeting is via CD8 alpha chain.DepositorInsertCD8A (CD8A Human)
UseTagsFKBP and GFPExpressionMammalianMutationTruncatedPromoterCMVAvailable sinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only
