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pCG_SARS-CoV1 C-V5-tag ORF7a

Plasmid

#179971

Purpose

Mammalian expression vector for SARS-CoV-1 ORF7a, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126.

Insert

SARS-CoV-1 ORF7a (ORF7a Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCG_SARS-CoV1 NSP7 C-V5-tag

Plasmid

#179963

Purpose

Mammalian expression vector for SARS-CoV-1 Nsp7, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126.

Insert

SARS-CoV-1 Nsp7 (ORF1ab Synthetic)

Use

Tags

Expression

Mammalian

Mutation

human codon-optimized

Promoter

Available since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pSPL3-hRPH3A_c.444Gwt

Plasmid

#190476

Purpose

minigene assay

Depositor

Alfredo Brusco

Article

Pavinato et al Genet Med. 2023 Nov;25(11):100922. doi:

Insert

RPH3A (NM_014954.3) c.444 [exon 7 and part of surrounding introns only] (RPH3A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pSPL3-hRPH3A_c.1853Awt

Plasmid

#190478

Purpose

minigene assay

Depositor

Alfredo Brusco

Article

Pavinato et al Genet Med. 2023 Nov;25(11):100922. doi:

Insert

RPH3A (NM_014954.3) c.1853 [exon 20 and part of surrounding introns only] (RPH3A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pITGA5_1745G>U

Plasmid

#173812

Purpose

mir218 binding site2 point mutation

Depositor

Andrew M. Siedlecki

Article

Liu et al J Am Soc Nephrol. 2022 Mar;33(3):565-582

Insert

NT1745 ITGA5 point mutation (ITGA5 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

AmpR

Available since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pITGA5_473G>A

Plasmid

#173811

Purpose

mir218 binding site1 point mutation

Depositor

Andrew M. Siedlecki

Article

Liu et al J Am Soc Nephrol. 2022 Mar;33(3):565-582

Insert

NT473 ITGA5 point mutation (ITGA5 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

AmpR

Available since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pMIR218-2_nt5G>U

Plasmid

#174009

Purpose

miR218 nt5 point mutation in SLIT3

Depositor

Andrew M. Siedlecki

Article

Liu et al J Am Soc Nephrol. 2022 Mar;33(3):565-582

Insert

SLIT3 (SLIT3, MIR218-2 Human)

Use

Tags

Expression

Mammalian

Mutation

NT5 G>U point mutation in SLIT3

Promoter

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pITGA5_Q853L

Plasmid

#174008

Purpose

Fibronectin receptor binding site point mutation

Depositor

Andrew M. Siedlecki

Article

Liu et al J Am Soc Nephrol. 2022 Mar;33(3):565-582

Insert

FibronR ITGA5 point mutation (ITGA5 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

AmpR

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shAtxn1

Plasmid

#180387

Purpose

Producing AAV that encodes mouse Atxn1 shRNA with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

Insert

shAtxn1 (Atxn1 Mouse)

Use

AAV and RNAi

Tags

Expression

Mutation

Promoter

CBA

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCG_batCoV RaTG13 ORF7a C-V5-tag

Plasmid

#179989

Purpose

Mammalian expression vector for RaTG13 ORF7a, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126.

Insert

RaTG13 ORF7a

Use

Tags

Expression

Mammalian

Mutation

human codon-optimized

Promoter

Available since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCG_batCoV RaTG13 NSP16 C-V5-tag

Plasmid

#179985

Purpose

Mammalian expression vector for RaTG13 Nsp16, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126.

Insert

RaTG13 Nsp16

Use

Tags

Expression

Mammalian

Mutation

human codon-optimized

Promoter

Available since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCG_SARS-CoV1 ORF6 C-V5-tag

Plasmid

#179970

Purpose

Mammalian expression vector for SARS-CoV-1 ORF6, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126.

Insert

SARS-CoV-1 ORF6 (ORF6 Synthetic)

Use

Tags

Expression

Mammalian

Mutation

human codon-optimized

Promoter

Available since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCG_SARS-CoV1 E C-V5-tag

Plasmid

#179966

Purpose

Mammalian expression vector for SARS-CoV-1 E, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126.

Insert

SARS-CoV-1 E (E Synthetic)

Use

Tags

Expression

Mammalian

Mutation

human codon-optimized

Promoter

Available since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCG_SARS-CoV1 NSP16 C-V5-tag

Plasmid

#179965

Purpose

Mammalian expression vector for SARS-CoV-1 Nsp16, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126.

Insert

SARS-CoV-1 Nsp16 (ORF1ab Synthetic)

Use

Tags

Expression

Mammalian

Mutation

human codon-optimized

Promoter

Available since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-2xFLAG-2xSTREP-CCND1(T286I)-IRES-mCherry

Plasmid

#172638

Purpose

Lentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D1(T286I) and mCherry in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND1 (CCND1 Human)

Use

Lentiviral

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Mutation

Thr286Ile

Promoter

CMV

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCV-FLAG-2xSTREP-CCND1(P287A)-Puro

Plasmid

#172641

Purpose

Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D1(P287A) and a puromycin resistance marker in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND1 (CCND1 Human)

Use

Retroviral

Tags

FLAG-2xSTREP

Expression

Mammalian

Mutation

Pro287Ala

Promoter

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV-FLAG-2xSTREP-CCND1(T286A)-Puro

Plasmid

#172642

Purpose

Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D1(T286A) and a puromycin resistance marker in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND1 (CCND1 Human)

Use

Retroviral

Tags

FLAG-2xSTREP

Expression

Mammalian

Mutation

Thr286Ala

Promoter

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-2xFLAG-2xSTREP-CCND1(P287S)

Plasmid

#172644

Purpose

Expresses 2xFLAG-2xSTREP-tagged cyclin D1(P287S) in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND1 (CCND1 Human)

Use

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Mutation

Pro287Ser

Promoter

CMV

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-2xFLAG-2xSTREP-CCND1(T286I)

Plasmid

#172645

Purpose

Expresses 2xFLAG-2xSTREP-tagged cyclin D1(T286I) in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND1 (CCND1 Human)

Use

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Mutation

Thr286Ile

Promoter

CMV

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-2xFLAG-2xSTREP-CCND1(P287L)

Plasmid

#172646

Purpose

Expresses 2xFLAG-2xSTREP-tagged cyclin D1(P287L) in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND1 (CCND1 Human)

Use

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Mutation

Pro287Leu

Promoter

CMV

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge