Addgene

Showing: 6261 - 6280 of 20469 results

Results per page:

Sort results by:

Neg. Ctrl_sgRNA

Plasmid

#111298

Purpose

CRISPR KO murine neg. ctrl.

Depositor

Vamsi Mootha

Article

Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

Insert

NegCtrl sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pPB-R1R2_EF1aVP64dCas9p300_T2A_MS2p65HSF1-IRESbsdpA

Plasmid

#113344

Purpose

Expresses VP64-dCas9-p300 and MS2-p65-HSF1 fusion proteins

Depositor

Gavin Wright

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

VP64-dCas9-p300core-T2A-MS2-p65-HSF1

Use

Piggybac

Tags

Expression

Mutation

Promoter

Available since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pOC2 CRISPIE-Halo-Homer1 KI

Plasmid

#183446

Purpose

CreON knock-in for Halo-Homer1 (amino acid position: G2)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and CRISPIE Halo donor

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
MsCLYBL_sgRNA1

Plasmid

#111294

Purpose

CRISPR KO murine CLYBL

Depositor

Vamsi Mootha

Article

Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

Insert

sgRNA1 against murine CLYBL

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLH-sgRNA1-MS2-PP7

Plasmid

#75392

Purpose

sgRNA1-MS2-PP7

Depositor

Thoru Pederson

Article

Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

Insert

sgRNA1-2XMS2-PP7

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pLBH546_Tet-Cas14b1Locus

Plasmid

#112503

Purpose

Expresses Cas14b1 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoter

Depositor

Jennifer Doudna

Article

Harrington et al Science. 2018 Oct 18. pii: science.aav42

Insert

Cas14b1

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Tet

Available since

Oct. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ131D

Plasmid

#69287

Purpose

Golden Gate entry vector; 1st gRNA under OsU3 promoter

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pLBH547_Tet-Cas14b2Locus

Plasmid

#112504

Purpose

Expresses Cas14b2 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoter

Depositor

Jennifer Doudna

Article

Harrington et al Science. 2018 Oct 18. pii: science.aav42

Insert

Cas14b2

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Tet

Available since

Oct. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Brp-T2A-QF2w-Hsp70-3XP3-dsRed

Plasmid

#141094

Purpose

Template plasmid for inserting QF2w into the Brp gene of Aedes aegypti with CRISPR/Cas9, to generate a pan-neuronal driver line.

Depositor

Carolyn McBride

Article

Zhao et al Cell Rep Methods. 2021 Jul 26;1(3):10004

Insert

bruchpilot

Use

CRISPR

Tags

Expression

Insect

Mutation

coding sequence downstream of CRISPR cut site, sy…

Promoter

Available since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSIBR004

Plasmid

#177667

Purpose

SIBR Intron 4 (Int4) interrupts the CDS of FnCas12a

Depositor

John van der Oost

Article

Patinios et al Nucleic Acids Res. 2021 Oct 6. pii: 6382

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

lacUV5

Available since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
MsCLYBL_sgRNA6

Plasmid

#111295

Purpose

CRISPR KO murine CLYBL

Depositor

Vamsi Mootha

Article

Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

Insert

sgRNA6 against murine CLYBL

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pU6_(Gluc)_INT(GenPurpClon)

Plasmid

#68433

Purpose

General Purpose cloning vector for "INT"-like constructs under U6-promoter expression.

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

INT construct

Use

CRISPR; Cloning vector for generating int-like co…

Tags

Expression

Mammalian

Mutation

Promoter

human U6

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pAC-U63-tgRNA-nlsBFP

Plasmid

#169029

Purpose

gRNA-marker vector contain a U6:3 promoter, gRNA scaffold, and a Ubi-mTagBFP-NLS marker.

Depositor

Chun Han

Article

Allen et al PLoS Biol. 2021 Jan 14;19(1):e3001061. d

Type

Empty backbone

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

U6:3

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pM115: CasRx control presgRNA

Plasmid

#166867

Purpose

U6-driven expression of control (non-targeting) presgRNA compatible with CasRx.

Depositor

Guei-Sheung Liu

Article

Chuang et al Front Cell Dev Biol. 2021 Jun 11;9:66787

Insert

non-targeting presgRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCBEclos-cbei2385-g2-opt

Plasmid

#118215

Purpose

CRISPR-mediated base editing in Clostridium

Depositor

Sheng Yang

Article

Li et al Biotechnol Bioeng. 2019 Feb 10. doi: 10.

Insert

Apobec1, Casp nickase, UG1-sgRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Ptm

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pFETCh_ONECUT2

Plasmid

#135738

Purpose

Donor vector for 3' FLAG tag of human ONECUT2

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ONECUT2 Homology arms (ONECUT2 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Expression

Mutation

Promoter

Available since

Jan. 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMOD6-pGRR-r11-r10-yeGFP

Plasmid

#74368

Purpose

pGRR NOR gate promoter responsive to gRNA-r11 and gRNA-r10. Integrates in the URA locus of W303 and recovers URA function.

Depositor

Eric Klavins

Article

Gander et al bioRxiv 041871

Insert

yeGFP

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

pGRR-r11-r10

Available since

April 4, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pJCC_039 FnCas12a

Plasmid

#171666

Purpose

For bacterial expression of FnCas12a with a C-terminal sorting motif and His tag

Depositor

Jennifer Doudna

Article

Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

Insert

FnCas12a

Use

Tags

6X His and sortase A sorting motif

Expression

Bacterial

Mutation

Promoter

Available since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLH-sgRNA1-boxB-MS2

Plasmid

#75396

Purpose

sgRNA1-boxB-MS2

Depositor

Thoru Pederson

Article

Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

Insert

sgRNA1-boxB-MS2

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pUAS-FRT-GFP-FRT-uM-dCBEevoCDA1

Plasmid

#205152

Purpose

Inducible expression of dCBEevoCDA1 under control of Gal4/UAS and FLP/FRT

Depositor

Michael Boutros

Article

Doll et al Sci Adv. 2023 Sep;9(35):eadj1568. doi: 1

Insert

FRT-GFP-FRT-uORF-dCBEevoCDA1

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

UAST

Available since

Sept. 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge