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Plasmid#174862PurposeExpresses mouse Peg10 cargoRNA encoding Cre recombinaseDepositorInsertCre flanked by MmPeg10 UTRs
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pCMV-RosaR4 KKR mutations
Plasmid#37199DepositorInsertFokI (fused to RosaR4 ZFN)
UseZfn expressionTagsHA, ROSA26 eZFNs R4, and SV40 NLSExpressionMammalianMutationI538K, E490K, and H537RPromoterCMVAvailable sinceJuly 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pUC gRNA Shuttle
Plasmid#47024PurposeEncodes a template from which gRNAs can be made via InFusion cloning. The Medicago truncatula U6.6 promoter drives the gRNA. For use in plants.DepositorInsertgRNA Shuttle
UseCRISPR; Cas9TagsExpressionPlantMutationG to T cloning mutation at position 323PromoterMedicago truncatula U6.6Available sinceSept. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IP TFEB-sfGFP
Plasmid#135402PurposeExpresses TFEB-sfGFPDepositorInsertTFEB (TFEB Human)
UseRetroviralTagssfGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
TERT - WT
Plasmid#213827PurposeExpresses human telomerase reverse transcriptase, the catalytic subunit of telomeraseDepositorInserthuman TERT (TERT Human)
UseLentiviralTagsExpressionMammalianMutationPromoterTet-responsive promoter PTight, consisting of sev…Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFUW-VenusFlag-hCD44
Plasmid#211824PurposeExpress CD44 in mammalian cellsDepositorInsertCD44 (CD44 Human)
UseLentiviralTagsN-Terminal Venus and Flag tagsExpressionMammalianMutationisoform corresponds to UniProt ID P16070-1 with a…PromoterAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAGD335
Plasmid#219499PurposepAGD248 derivative with the retron Eco1 msr, sfGFP flanked by 2 BsaI sites , and the rest of the retron recombineering operon (Eco1 RT, CspRect, MutL-E32K) downstream of the pBad promoterDepositorInsertEco1 msr, sfGFP, Eco1 RT, CspRecT, MutLE32K
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pC0078 RESCUE
Plasmid#130661PurposepCMV-dCas13b6-mapkNES-GS-dADAR2(E488Q/V351G/S486A/T375S/S370C/P462A/N597I/L332I/I398V/K350I/M383L/D619G/S582T/V440I/S495N/K418E/DepositorInsertdRanCas13b-ADAR2dd (RESCUE)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX330A-FBL/PITCh
Plasmid#63671PurposeExpresses Cas9 nuclease, FBL-specific gRNA, and the PITCh-gRNADepositorInserthumanized S. pyogenes Cas9
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailable sinceDec. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMAL358
Plasmid#169147PurposeEncodes OptoQ-AMP1DepositorInsertVP16-EL222
UseTagsSV40 NLSExpressionYeastMutationPromoterAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHIPH4
Plasmid#117685PurposeHansenula polymorpha expression plasmidDepositorInsertAlcohol Oxidase promoter
UseTagsExpressionYeastMutationPromoterAlcohol OxidaseAvailable sinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAW016-2
Plasmid#85613PurposeCas9-tracrRNA integration vector for Bacillus subtilisDepositorInsertcas9-tracrRNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLV hUbC-dSaCas9-KRAB-T2A-PuroR
Plasmid#106249PurposeLentiviral vector with puro selection for expression of S. aureus dCas9-KRABDepositorInsertdead S. aureus Cas9 KRAB T2A PuroR
UseCRISPR and LentiviralTagsHA-NLS and NLS-KRABExpressionMammalianMutationD10A and N580APromoterhUbCAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
p-EGFP-C1-Flag-mFmr1(wt)
Plasmid#87929PurposeExpresses mouse FMRP-EGFP fusion, which can be phosphorylated on ser-499.DepositorInsertFmr1 (Fmr1 Mouse)
UseTagsFlag and eGFPExpressionMammalianMutationNonePromoterAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
p37-2iDMD-LR
Plasmid#88892Purpose37°C full-length DMD luc-red plasmid. Co-expresses human dystrophin, firefly luciferase and mCherry in mammalian cells. Plasmid carries phiC31 and Bxb1 attB sites and can be grown at 37°C.DepositorInsertsUseTagsExpressionMammalianMutationPromoterAvailable sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-Sec61b-C1
Plasmid#90992PurposeExpresses mEmerald-tagged Sec61b, brightly labels endoplasmic reticulum in mammalian cellsDepositorInsertmEmerald-Sec61b (SEC61B Human, Synthetic)
UseTagsmEmerald (green fluorescence)ExpressionMammalianMutationPromoterCMVAvailable sinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
IDG_CACNA2D2_OE_1
Plasmid#161616PurposeOverexpresses CACNA2D2DepositorInsertCACNA2D2 (CACNA2D2 Human)
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUbC-tdTomato
Plasmid#183710PurposetdTomato expression from UbiC promoterDepositorInserttdTomato
UseLentiviralTagsExpressionMutationPromoterUbiCAvailable sinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_IDH1_p.R132H
Plasmid#81686PurposeGateway Donor vector containing IDH1, part of the Target Accelerator Plasmid Collection.DepositorInsertIDH1 (IDH1 Human)
UseGateway entry vectorTagsExpressionMutationR132HPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only