Showing: 6241 - 6260 of 16015 results
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Plasmid#183232PurposeLentiviral vector; Tet-on advanced system driving the expression of tagged HNF1ADepositorInsertHNF1 homeobox A (HNF1A Human)
UseLentiviralTagsAviTag and FLAG tagExpressionMutationPromotertight TREAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ICUE4
Plasmid#181846PurposeFourth-generation FRET-based Indicator of cAMP Using Epac. Genetically encoded cAMP indicator. Contains high-sensitivity Epac Q270E mutation.DepositorInsertICUE4 (RAPGEF3 Human)
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationGlutamine 122 in Epac1 domain mutated to glutamat…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pOPINB-HOIL-1 helix-RBR (233–510)
Plasmid#193859PurposeExpression of human His-tagged HOIL-1 (RBCK1) helix-RBR (aa 233–510) codon optimized for E. coliDepositorInsertHOIL-1 (RBCK1 Human)
UseTags3C protease cleavage site and 6x-His tagExpressionBacterialMutationResidues 233–510, codon optimised for expression …PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pETM11-SUMO3-KSR1-CA3-sfGFP (C-KSR)
Plasmid#217766PurposeFluorescent reporter for ceramide (for bacterial expression and purification)DepositorInsertKinase suppressor of ras 1 CA3 domain (KSR1 Human)
UseProtein purification (6xhis tag, tev site, sumo3 …TagssfGFPExpressionBacterialMutationCA3 domain aa 317-400 plus vector-based linker LE…PromoterT7 promoterAvailabilityIndustry, Academic Institutions, and Nonprofits -
pETM11-SUMO3- sfGFP-KSR1-CA3 (N-KSR)
Plasmid#217767PurposeFluorescent reporter for glucosyl-ceramide (putative, mammalian expression)DepositorInsertKinase suppressor of ras 1 CA3 domain (KSR1 Human)
UseProtein purification (6xhis tag, tev site, sumo3 …TagssfGFPExpressionBacterialMutationCA3 domain aa 317-400PromoterT7 promoterAvailabilityIndustry, Academic Institutions, and Nonprofits -
EF1a-L274GMmMetRS-T2A-mCherry
Plasmid#220800PurposeLentivirus expressing mutant tRNA synthetase for incorporation of noncanonical amino acids in nascent proteins plus mCherry reporter and puro resistanceDepositorInsertMars1 (Mars1 Mouse)
UseLentiviralTags2xFLAG and T2A-mCherryExpressionMutationmutation in MetRS to change aa 274 from L to GPromoterEF1aAvailabilityAcademic Institutions and Nonprofits only -
pNX3a-PK9
Plasmid#78356Purposefission yeast epitope taggingDepositorInserts9xPK
kanMX6
UseTagsExpressionYeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pET-30 Ubwt (2)
Plasmid#26685DepositorInsertUbiquitin
UseTagsHis and S-tagExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
6011 pETH6K620Tb
Plasmid#1240DepositorInsertHsp104 (K620T mutant) (HSP104 Budding Yeast)
UseTagsHis tagExpressionBacterialMutationK620TPromoterAvailabilityAcademic Institutions and Nonprofits only -
6107 pETH6KT218
Plasmid#1241DepositorInsertHsp104 (K218T) (HSP104 Budding Yeast)
UseTagsHis tagExpressionBacterialMutationK218TPromoterAvailabilityAcademic Institutions and Nonprofits only -
pFA6a-hphMX-tetO-Pcyc1-3xFLAG
Plasmid#41020DepositorInserttetO7-Pcyc1-3xFLAG
UseN-terminal tagging/promoter replacementTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Gi1-CASE
Plasmid#168120PurposeEncodes a BRET-based activity sensor for the heterotrimeric G protein Gi1. Composed of the subunits G alpha i1 (GNAI1) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).DepositorUseLuciferaseTagscpVenus on GNG2ExpressionMammalianMutationNLuc is inserted at A121/E122 within GNAI1PromoterAvailabilityAcademic Institutions and Nonprofits only -
Gi3-CASE
Plasmid#168122PurposeEncodes a BRET-based activity sensor for the heterotrimeric G protein Gi3. Composed of the subunits G alpha i3 (GNAI3) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).DepositorUseLuciferaseTagscpVenus on GNG2ExpressionMammalianMutationNLuc is inserted at A114/E115 within GNAI1PromoterAvailabilityAcademic Institutions and Nonprofits only -
G15-CASE
Plasmid#168126PurposeEncodes a BRET-based activity sensor for the heterotrimeric G protein G15. Composed of the subunits G alpha 15 (GNA15) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).DepositorUseLuciferaseTagscpVenus on GNG9ExpressionMammalianMutationNLuc is inserted at E244/N245 within GNA15PromoterAvailabilityAcademic Institutions and Nonprofits only -
pTripz NEO DEST
Plasmid#209072PurposeModified DOX-ON inducible destination vector with a c-terminal 3X flag tag to be used for LR recombination reactions to inducibly express cDNAs of interestDepositorTypeEmpty backboneUseLentiviral; Gateway destination vector to be used…Tags3X FLAGExpressionMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pNX3a-PK3
Plasmid#78346Purposefission yeast epitope taggingDepositorInserts3xPK
kanMX6
UseTagsExpressionYeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pNX3a-PK6
Plasmid#78355Purposefission yeast epitope taggingDepositorInserts6xPK
kanMX6
UseTagsExpressionYeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pNX3a-PK12
Plasmid#78357Purposefission yeast epitope taggingDepositorInserts12xPK
kanMX6
UseTagsExpressionYeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pNX3a-FL3
Plasmid#78358Purposefission yeast epitope taggingDepositorInserts3xFLAG
kanMX6
UseTagsExpressionYeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pNX3a2-PK3
Plasmid#78382Purposefission yeast epitope taggingDepositorInserts3xPK
neoCV
UseTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 6241 - 6260 of 16015 results