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mCherry-C1-ORP5-H478-9A-deltaTMD-FKBP

Plasmid

#220075

Purpose

expresses a mutant ORP5 that is unable to bind to PI4P with an mCherry fluorophore and an FKBP

Depositor

Gerry Hammond

Article

Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

Insert

OSBPL5 H478/9A (OSBPL5 Human)

Use

Tags

Expression

Mammalian

Mutation

H478/9A, deltaTMD

Promoter

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-C1-ORP5deltaORDdeltaTMD-FKBP

Plasmid

#220074

Purpose

expresses ORP5 with the OSBP-related ligand-binding domain and the transmembrane domain deleted. The mCherry fluorophore and FKBP are attached to ORP5.

Depositor

Gerry Hammond

Article

Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

Insert

OSBPL5deltaORDdeltaTMD (OSBPL5 Human)

Use

Tags

Expression

Mammalian

Mutation

deltaORDdeltaTMD

Promoter

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pTagBFP-C1-FKBP-PIP5K1C-HD-EE

Plasmid

#220090

Purpose

Recruitable PIP5K to make PIP2

Depositor

Gerry Hammond

Article

Weckerly et al bioRxiv [Preprint]. 2024 Feb 28:2024.02.

Insert

PIP5K1C (PIP5K1C Human)

Use

Tags

TagBFP-FKBP1A(3-108)

Expression

Mammalian

Mutation

amino acids 1-635; D101R, R304D, R445E, K446E

Promoter

CMV

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pNeonGreen-N1-Nir1-613-630

Plasmid

#220088

Purpose

N-terminal amphipathic helix of Nir1-LNS2 domain; loses ability to bind PA

Depositor

Gerry Hammond

Article

Weckerly et al bioRxiv [Preprint]. 2024 Feb 28:2024.02.

Insert

PITPNM3 (PITPNM3 Human)

Use

Tags

GGSGG-NeonGreen

Expression

Mammalian

Mutation

amino acids 613-630

Promoter

CMV

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pNeonGreen-C1-Nir2-LNS2

Plasmid

#220086

Purpose

C-terminal PA binding domain of Nir2

Depositor

Gerry Hammond

Article

Weckerly et al bioRxiv [Preprint]. 2024 Feb 28:2024.02.

Insert

PITPNM1 (PITPNM1 Human)

Use

Tags

NeonGreen-GGSGGM

Expression

Mammalian

Mutation

amino acids 896-1216

Promoter

CMV

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pNES-EGFP-C1-flex-PABD-Spo20

Plasmid

#220081

Purpose

Spo20-PABD PA biosensor with a flexible linker to aid in nuclear export

Depositor

Gerry Hammond

Article

Weckerly et al bioRxiv [Preprint]. 2024 Feb 28:2024.02.

Insert

Spo20 (SPO20 Budding Yeast)

Use

Tags

X. leavis map2k1.L(32-44)-EGFP-SGGGSGGS

Expression

Mammalian

Mutation

amino acids 51-91

Promoter

CMV

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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Lneg-INTR-GFP

Plasmid

#208902

Purpose

encodes the inotocin receptor (oxytocin/vasopressin-like) from the ant Lasius neglectus, tagged with GFP

Depositor

Christian Gruber

Article

Liutkeviciute et al FASEB J. 2018 Jun 25;32(12):fj201800443.

Insert

INTR

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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rSirt3 shRNA (pLKO.1 vector)

Plasmid

#216807

Purpose

shRNA against rat Sirt3

Depositor

Ghazaleh Ashrafi

Article

Tiwari et al J Cell Biol. 2024 Jan 1;223(1):e20230504

Insert

Sirt3 (Sirt3 Rat)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-kcnk3a

Plasmid

#208985

Purpose

Riboprobe generation

Depositor

GuangJun Zhang

Article

Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

Insert

kcnk3a (kcnk3a Zebrafish)

Use

Cloning

Tags

Expression

Mutation

Promoter

T7

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR-D-Topo-kcnk5a

Plasmid

#208988

Purpose

Riboprobe generation

Depositor

GuangJun Zhang

Article

Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

Insert

kcnk5a (kcnk5a Zebrafish)

Use

Cloning

Tags

Expression

Mutation

Promoter

T7

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-kcnk6

Plasmid

#208990

Purpose

Riboprobe generation

Depositor

GuangJun Zhang

Article

Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

Insert

kcnk6 (kcnk6 Zebrafish)

Use

Cloning

Tags

Expression

Mutation

Promoter

T7

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR-D-Topo-kcnk9

Plasmid

#208992

Purpose

Riboprobe generation

Depositor

GuangJun Zhang

Article

Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

Insert

kcnk9 (kcnk9 Zebrafish)

Use

Cloning

Tags

Expression

Mutation

Without STOP codon

Promoter

T7

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-kcnk13a

Plasmid

#208996

Purpose

Riboprobe generation

Depositor

GuangJun Zhang

Article

Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

Insert

kcnk13a (kcnk13a Zebrafish)

Use

Cloning

Tags

Expression

Mutation

Promoter

T7

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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NSP3C-FR-2Strep

Plasmid

#210312

Purpose

Expresses the C terminal of SARS-CoV-2 NSP3 in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

C terminal of SARS-CoV-2 NSP3 (ORF1ab )

Use

Tags

2Strep

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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NSP3C-EGFP

Plasmid

#210318

Purpose

Expresses the C terminal of SARS-CoV-2 NSP3 fused with EGFP tag in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

C terminal of SARS-CoV-2 NSP3 (ORF1ab )

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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SARS-CoV-2 N-2Strep

Plasmid

#210376

Purpose

Expresses SARS-CoV-2 N protein fused with 2Strep in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

SARS-COV-2 N (N SARS-CoV-2)

Use

Tags

2Strep

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pDN0617 (pDEST-mEGFP C-term, NatR)

Plasmid

#210484

Purpose

pDEST vector to tag gene of interest with mEGFP on the C-terminus to perform localization assays in S. cerevisiae.

Depositor

Christian Landry

Article

Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

Type

Empty backbone

Use

Synthetic Biology; Destination vector for gateway…

Tags

mEGFP

Expression

Yeast

Mutation

Promoter

ADH1

Available since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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Combined γB2FL-FRET probe

Plasmid

#209094

Purpose

mTurquoise2- and mVenus-inserted PcdhgB2 full length (FL)

Depositor

Takeharu Nagai

Article

Hoshino et al Proc Natl Acad Sci U S A. 2023 Sep 19;12

Insert

PcdhgB2 (Pcdhgb2 Mouse)

Use

Tags

PA tag

Expression

Mammalian

Mutation

Promoter

Chicken β-actin

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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Combined γB2ΔICD-FRET probe

Plasmid

#209093

Purpose

mTurquoise2- and mVenus-inserted PcdhgB2 lacking an intracellular domain (ICD)

Depositor

Takeharu Nagai

Article

Hoshino et al Proc Natl Acad Sci U S A. 2023 Sep 19;12

Insert

PcdhgB2 (Pcdhgb2 Mouse)

Use

Tags

PA tag

Expression

Mammalian

Mutation

Promoter

Chicken β-actin

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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REEP6-2Strep

Plasmid

#210351

Purpose

Expresses REEP6 fused with 2Strep in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

REEP6 (REEP6 Human)

Use

Tags

2Strep

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge