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Showing: 6221 - 6240 of 16015 results

  1. pINDUCER20-FLAG-hMYF5 (#801)

    Plasmid
    #78334
    Purpose
    Doxycycline-inducible lentiviral overexpression of human MYF5 ORF with a FLAG tag
    Depositor
    Louis Kunkel, Matthew Alexander
    Insert
    MYF5 (MYF5 Human)
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mutation
    Promoter
    TRE2
    Availability
    Academic Institutions and Nonprofits only

  2. pINDUCER20-FLAG-hSRF (#799)

    Plasmid
    #78345
    Purpose
    Doxycycline-inducible lentiviral overexpression of human SRF ORF with a FLAG tag
    Depositor
    Louis Kunkel, Matthew Alexander
    Insert
    SRF (SRF Human)
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mutation
    Promoter
    TRE2
    Availability
    Academic Institutions and Nonprofits only

  3. pCAG-Scarlet-Kif5a

    Plasmid
    #118745
    Purpose
    Expresses scarlet tagged Kif5a
    Depositor
    Beverly Koller
    Insert
    Kif5a cDNA (Kif5a Mouse)
    Use
    Tags
    mScarlet
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Availability
    Academic Institutions and Nonprofits only
    118745-plasmid-map-sequence-id-229089

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  4. pCAG-Scarlet-Borcs7

    Plasmid
    #118750
    Purpose
    Expresses scarlet tagged Borcs7-Q87X
    Depositor
    Beverly Koller
    Insert
    Borcs7-Q87X cDNA (2010012O05Rik Mouse)
    Use
    Tags
    mScarlet
    Expression
    Mammalian
    Mutation
    Q87X
    Promoter
    CAG
    Availability
    Academic Institutions and Nonprofits only
    118750-plasmid-map-sequence-id-229084

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  5. MAPK8_BirA_Cterm

    Plasmid
    #221519
    Purpose
    Flag-tagged biotinylation enzyme for co-expression of Mapk8
    Depositor
    Alessandro Ori
    Insert
    MAPK8 (MAPK8 Human)
    Use
    Tags
    BirA
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    221519-plasmid-map-sequence-id-439379

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  6. MAPK9_BirA_Cterm

    Plasmid
    #221520
    Purpose
    Flag-tagged biotinylation enzyme for co-expression of Mapk9
    Depositor
    Alessandro Ori
    Insert
    MAPK9 (MAPK9 Human)
    Use
    Tags
    BirA
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    221520-plasmid-map-sequence-id-439222

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  7. MAPK9_BirA_Nterm

    Plasmid
    #221521
    Purpose
    Flag-tagged biotinylation enzyme for co-expression of Mapk9
    Depositor
    Alessandro Ori
    Insert
    MAPK9 (MAPK9 Human)
    Use
    Tags
    BirA
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    221521-plasmid-map-sequence-id-439409

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  8. pCRISPaint-mScarlet-F-gB-PuroR [M1G]

    Plasmid
    #171812
    Purpose
    Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, HSV-1 glycoprotein B CD8+ T cell epitope [AA: 498-505 (SSIEFARL)] and puromycin resistance cassette [p.M1
    Depositor
    Michael Hoelzel
    Article
    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.
    Insert
    mScarlet-F-gB-T2A-PuroR
    Use
    Gene tagging
    Tags
    Expression
    Mutation
    PuroR: Changed Methionin 1 to Glycine.
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    171812-plasmid-map-sequence-id-337985

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  9. pCRISPaint-mNeon-F-gB-PuroR [M1G]

    Plasmid
    #171803
    Purpose
    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, HSV-1 glycoprotein B CD8+ T cell epitope [AA: 498-505 (SSIEFARL)] and puromycin resistance cassette [p.M1G]
    Depositor
    Michael Hoelzel
    Article
    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.
    Insert
    mNeonGreen-FLAG-gB-T2A-PuroR
    Use
    Gene tagging
    Tags
    Expression
    Mutation
    PuroR: Changed Methionin 1 to Glycine.
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    171803-plasmid-map-sequence-id-341955

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  15. pTO_CHMP3_LAP

    Plasmid
    #101849
    Purpose
    Tet-inducible expression of human CHMP3 with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integration
    Depositor
    Daniel Gerlich
    Article
    Mierzwa et al Nat Cell Biol. 2017 Jul;19(7):787-798. d
    Insert
    Charged Multivesicular Body Protein 3 (CHMP3 Human)
    Use
    Gateway cloning, flp-frt recombination, tet-induc…
    Tags
    Localization and Affinity Purification (LAP) tag
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    101849-plasmid-map-sequence-id-200249

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  16. pET-28b-T7-hAsCas12a-NLS(nucleoplasmin)-6xHis (BPK3541)

    Plasmid
    #114069
    Purpose
    T7 promoter bacterial expression plasmid for human codon optimized AsCas12a with C-terminal NLS and His-tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized AsCas12a with C-terminal NLS and His-tag
    Use
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    114069-plasmid-map-sequence-id-220520

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  17. pET-28b-T7-bLbCas12a-NLS(nucleoplasmin)-6xHis (RTW645)

    Plasmid
    #114070
    Purpose
    T7 promoter bacterial expression plasmid for bacterial codon optimized LbCas12a with C-terminal NLS and His-tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    bacteria codon optimized LbCas12a with C-terminal NLS and His-tag
    Use
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    114070-plasmid-map-sequence-id-220511

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  19. pDNA2.0 6H-TEV-KRAS G13D

    Plasmid
    #111851
    Purpose
    Expresses activated human KRAS G13D (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.
    Depositor
    Kenneth Westover
    Article
    Hunter et al Mol Cancer Res. 2015 Sep;13(9):1325-35.
    Insert
    KRAS G13D (KRAS Human)
    Use
    Tags
    6xHis-tag and TEV protease cleavage sequence
    Expression
    Bacterial
    Mutation
    Changed Glycine 13 to Aspartic Acid
    Promoter
    T5
    Availability
    Academic Institutions and Nonprofits only
    111851-plasmid-map-sequence-id-213465

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Showing: 6221 - 6240 of 16015 results