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Showing: 6221 - 6240 of 11044 results

  3. pCI-Flag-NEDD4L∆WW1,2,4

    Plasmid
    #171137
    Purpose
    expresses NEDD4L with inactivating mutation in first, second and fourth WW domain, but intact third WW domain, in mammalian cells
    Depositor
    Wesley Sundquist
    Article
    Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10
    Insert
    NEDD4L (NEDD4L Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    changed tryptophan 221 to alanine, tryptophan 453…
    Promoter
    Available since
    July 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    171137-plasmid-map-sequence-id-338242

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  12. pPapi

    Plasmid
    #96921
    Purpose
    expression of S aureus SaCas9 (SauCas9) and two pol III promoters for an Sa gRNA and an Sp gRNA. Intended to be used in SpCas9-expressing cell lines for dual Cas9 "Big Papi" screens. aka pXPR207.
    Depositor
    John Doench, David Root
    Article
    Najm et al Nat Biotechnol. 2018 Feb;36(2):179-189.
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    96921-plasmid-map-sequence-id-181715

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  13. GFP-PHIP

    Plasmid
    #65389
    Purpose
    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.
    Depositor
    Kyle Miller
    Article
    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do
    Insert
    PHIP (PHIP Human)
    Use
    Tags
    EmGFP and V5
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    May 14, 2015
    Availability
    Academic Institutions and Nonprofits only

  20. HA-IFITM3-F8-TAG-C72A

    Plasmid
    #122477
    Purpose
    expresses human IFITM3-C72A with unnatural amino acid incorporated site specifically in response to the amber codon TAG in mammalian cells when co-transfected with tRNA synthetase/tRNA pair.
    Depositor
    Howard Hang
    Article
    Spence et al Nat Chem Biol. 2019 Jan 14. pii: 10.1038
    Insert
    IFITM3 (IFITM3 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Changed Phenylalanine 8 to amber codon, cysteine …
    Promoter
    Available since
    March 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    122477-plasmid-map-sequence-id-237728

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Showing: 6221 - 6240 of 11044 results